LUMIstar Omega

Специализированный планшетный люминометр

LUMIstar^® Omega - это специализированный планшетный люминометр, который может измерять и быструю и затухающую люминесценцию. Прибор имеет сертификат DLReady и может быть модернизирован для проведения одновременных измерений на двух длинах волн для приложений BRET, так же в дальнейшем возможна модернизация до мультимодального ридера.

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Режимы обнаружения

Absorbance
Fluorescence intensity (incl. FRET)
Luminescence (incl. BRET)
Fluorescence polarization
AlphaScreen
Nephelometry
Time-resolved fluorescence
TR-FRET

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LUMIstar Omega

LUMIstar Omega - это специализированный ридер для измерения люминесценции, включая BRET. Он обеспечивает измерения с высокой точностью быстрой и затухающей люминесценции в кинетике и по конечной точке. Более того, встроенное колесо фильтров и возможность модернизации до одновременной детекции на двух длинах волн позволяют работать с наборами BRET.

Характеристики

Методики BRET, с быстрой и с затухающей люминесценцией.
Измериения в плоть до 384-луночных планшет
Измерения сверху и снизу
Инкубация до 45С или 65С
Одновременное измерение на двух длинах волн испускания
До 900 точек данных при сканировании лунки
Детекция в момент инжекции

Технологии считывания микропланшетов

Gas Vent

BMG LABTECH offers two forms of gas control on its microplate readers. The Atmospheric Control Unit (ACU) for the CLARIOstar is a unique microprocessor-controlled unit that independently regulates both CO2 and O2 inside the microplate reader. Alternatively, the Gas Vent is a positive pressure gas purge through which the internal atmosphere of the microplate reader can be replaced.

Incubation and shaking

Shaking and incubation allow users to mix the well content and easily run measurements at elevated temperatures. The incubator controls the air temperature in the measurement chamber from room temperature up to 45°C, 60°C or 65°C. BMG LABTECH microplate readers are equipped with orbital, double-orbital or linear shaking with definable intensity, duration and direction of motion. These features are available on all BMG LABTECH plate readers.

Path length correction

Absorbance measurements such as DNA quantification or protein determination detected in a microplate reader are frequently compared and/or normalized to results from a 1 cm path length cuvette. All BMG LABTECH microplate readers have the ability to perform a 1 cm path length correction thanks to an internal fitting algorithm. Using this correction, data normalization to 1 cm path is given with only one mouse click.

Reagent injectors

Many of today's most popular assays such as enzyme kinetics, calcium flux, and dual luciferase require the ability to monitor a signal during and after the addition of a reagent. All BMG LABTECH readers, with the exception of the SPECTROstar Nano, can be equipped with up to two reagent injectors. Injectors enable reagent delivery to any plate format from 6 to 384 wells.

Simultaneous Dual Emission

Simultaneous Dual Emission (SDE) detection allows the measurement of two emitted wavelengths at the same time. It reduces plate read times and corrects for any signal variation due to differences in well volumes, concentrations, or fluctuations in excitation energy. SDE is the ideal tool for double-emission assays such as FRET, TR-FRET, fluorescence polarization and BRET, since the sample only has to be read once.

UV/vis spectrometer

BMG LABTECH was the first and is the only microplate reader manufacturer to equip its instruments with a UV/vis spectrometer for absorbance measurements. Similar to a monochromator, but much faster, the spectrometer will capture full-absorbance spectra from 220 to 1000 nm at a resolution of 1 nm in less than one second/well. Alternatively, up to eight discrete wavelengths can be collected at the same time.

Well-scanning

For non-homogeneous samples such as for cell-based assays, where the sample distribution is uneven across the well, a single reading in the centre of the well is inadequate. To overcome this problem, all BMG LABTECH readers come with an advanced Well Scanning feature that can take multiple measurements in each well with a resolution of up to 900 points/well. Alternatively, in spiral or orbital averaging mode, the reader takes several measurements for each well on a defined spiral or orbit and calculates an average.

Xenon Flash lamps

Microplate readers require a light source to provide the incident of light directed at the sample.

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Differential activation of G proteins by synthetic cannabinoid receptor agonists, utilizing the CAMYEL BRET biosensor

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Real-Time Cell Motility Tracking Increases the Throughput of Scratch Wound Assays

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NextGenPCR™ evaluated with a fluorescence microplate reader accelerates testing for SARS-CoV-2 viral RNA

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