Molecular biology deals with biomolecules, their structure, function, and interactions. Since the discovery and description of DNA in the 1950s, we came to understand how DNA gets first transcribed into RNA and then translated into proteins. We further understand that the genome is not the only basis for a cell’s behavior, differentiation, and pathology. Epigenetic regulation, transcription regulation and non-coding RNAs are just as important.
Today, microplate reader assays are well-established tools to answer the questions that are still open. Luminescent reporter assays study gene regulation, interaction assays examine binding of nucleic acids to proteins and enzyme activity assays report on activities of polymerases or helicases.
Genomics is a multidisciplinary and essential part of modern biology. Next generation sequencing (NGS) and SNP-genotyping are popular tools. The field is also closely associated with transcription and translation, investigating proteins and expression levels, both to directly inform studies but also as part of analysis workflows such as NGS.
Microplate reader also take measurements in preparation for PCRs and sequencing. They quantify nucleic acids in high throughput (96-1536 wells), e.g. with NextGenPCRTM, and determine nucleic acid purity via absorbance spectra.
BMG LABTECH microplate readers offer solutions from simple quantification devices able to quantify DNA, RNA and protein efficiently and in high throughput, up to multi-functional devices suitable for all needs: reporter assays, binding assays, enzyme assays, evaluation of transfection efficiency, no matter which detection mode.
Search our resources section for information about specific applications, literature citations, videos, blog articles and many other publications. Many of the resources provided are associated with current and previous instrument models and versions.
Transfection efficiency cannot only be assessed with a microscope. Read how microplate reader-based evaluation can be achieved with high accuracy with the VANTAstar.
We compared DNA quantification methods: A260, fluorescent high sensitivity and broad range kits. Find out which method fits best to your dsDNA samples!
The novel fluorescent biosensor SSB quantifies RNA and assists in studying transcription. Read how the CLARIOstar microplate reader assisted in assay development.
Fluorescence polarization detection with the CLARIOstar® microplate reader to investigate influenza virus’s RNA polymerase activity is highly sensitive and HTS-compatible. Read more here.
Read here how the PHERAstar® FSX microplate reader identified small molecule inhibitors of the YEATS domain out of thousands of compounds using an AlphaScreen-based assay.
Read how GPCR interaction and trafficking are studied at endogenous expression levels by employing CRISPR/Cas9 and the CLARIOstarPlus, including its multiplexing capabilities.
Read how various nucleic acid quantification methods, ranging from UV absorbance and fluorescent dyes to amplification methods, can be performed on BMG LABTECH microplate readers.
In this blog you will learn what, why and how DNA interacts with different biomolecules and how microplate readers can help in their investigation.
Want to analyse complex cellular signalling events with minimal effort? Multiplexing luciferase assays enable multiple, highly quantitative measurements in a single experiment.
Histone deacetylases (HDACs) are becoming increasingly important. Our article explains the basics of this class of enzymes.
Abo-Zaid, G;Abdelkhalek, A;Matar, S;Darwish, M;Abdel-Gayed, M; 
Journal of fungi (Basel, Switzerland) 7(3)
Zhang, Y;Niu, G;Flisikowska, T;Schnieke, A;Flisikowski, K; 
Biochemical and biophysical research communications 553 25-29
Jung, S;Woo, C;Fugaban, JII;Vazquez Bucheli, JE;Holzapfel, WH;Todorov, SD; 
Probiotics and antimicrobial proteins
Ali, FEM;Elfiky, M;Fadda, WA;Ali, HS;Mahmoud, AR;Mohammedsaleh, ZM;Abd-Elhamid, TH; 
Life sciences 119433
Wong, KY;Morgan, GJ;Boyle, EM;Cheng, ASL;Yip, KY;Chim, CS; 
Scientific reports 11(1) 7009
Tellegen, A;Beukers, M;Rudnik-Jansen, I;van Klaveren, N;How, K;Woike, N;Mihov, G;Thies, J;Teske, E;Creemers, L;Tryfonidou, M;Meij, B; 
Pharmaceutics 13(3) 372
Alfredo Castello-Palomares from Oxford University talks about identifying and characterizing cellular RNA-binding proteins required for viruses to spread.
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Absorbance plate reader with cuvette port