
NanoBRET
NanoBRET is used to analyse binding events, signaling pathways and receptor trafficking in live cells and has significantly expanded the range and applications of BRET assays.
Mitochondria are one of the main places in cells where energy is produced via aerobic respiration.
Mitochondrial toxicity can occur when drugs or diseases affect the mitochondria and prevent the proper production of energy via oxygen consumption. Therefore a method is needed to test mitochondrial function and toxicity, especially when trying to design potential new drugs.
One way to measure mitochondrial function is to measure oxygen consumption, but traditional methods are limited by low throughput, by using specialized equipment, and by measuring only extracellular oxygen.
The MitoXpress®-Xtra assay from Agilent overcomes these limitations by providing a phosphorescent-based method that can measure intracellular oxygen consumption using the time-resolved detection found in many multi-mode microplate readers. When used with the Atmospheric Control Unit (ACU) on the FLUOstar Omega, VANTAstarTM, and CLARIOstar Plus® a real-time monitoring system can be created that measures the change in intracellular oxygen in a cell monolayer (see Application Note 223).
Find out more about the MitoXpress®-Xtra assay on our website.
NanoBRET is used to analyse binding events, signaling pathways and receptor trafficking in live cells and has significantly expanded the range and applications of BRET assays.
Find out about the different types of cell-based assays and why they have become an indispensable tool in a such a broad variety of disciplines in this blog article.
Read here about the various threats associated with mycoplasma contamination and find out about methods to prevent, detect, and eliminate mycoplasma in your cell culture.
Apoptosis is a form of programmed cell death responsible for the removal of damaged or unnecessary cells. Read more about available apoptosis assays here.
Calcium is one of the central players in biology. This blog explains some of the many roles of calcium and discusses the different ways that calcium ions can be measured on microplate readers.
This article highlights the characterisation of hemoglobin and how microplate readers can help to monitor its state or condition based on variations in its absorbance spectrum.