
Cell-based studies provide information about basic cellular processes. These include cell division, cell energy balance, control mechanisms, cell death regulation, differentiation or pathology. Cell-based methods are not only used for research: toxicity tests replace animal experiments and identify toxic substances in vitro. In addition, cell cultures have become important production organisms for medicines. For example, they produce insulin or monoclonal antibodies used as cancer therapeutics.
Modern cell biology analyses not only measure at a single time point but monitor biological changes over days in real time. To this end, the measurement chamber of a microplate reader needs controlled temperature and gas conditions. BMG LABTECH´s Atmospheric Control Unit regulates CO2 to stabilize the pH in carbonate buffered cell cultures. Additionally, O2 can be set to physiological conditions, which for mammalian cultures is typically below ambient concentrations. BMG LABTECH microplate readers further correct for non-homogenous distribution of a cell layer as they measure several points across the well instead of only reading in the center. To avoid biased results due to a undetected mycoplasma contamination, BMG LABTECH readers also offer the possibility of detecting existing contamination.
Setting up a cell biology experiment takes time. Therefore, novel assay solutions measure several parameters at once. The combination of different fluorescent, luminescent and chromogenic assays in one well results in multiple readouts. With a flexible microplate reader, all assays can be measured with only one instrument.
Search our resources section for information about specific applications, literature citations, videos, blog articles and many other publications. Many of the resources provided are associated with current and previous instrument models and versions.
Not sure which cell viability assay is the best choice for you? This AppNote compares different kits based on absorbance, fluorescence and luminescence readouts.
Are you looking for a label-free method to study potential antiviral drugs? This AppNote presents a fast and cheap way to study viral cytopathic effects with cell viability/toxicity assays.
This application note highlights the use of THUNDER™ cellular kinase assays designed to enable the measurement of endogenously expressed phosphorylated proteins.
Looking for an easy way to normalise your cell-based assays? This AppNote describes how Seahorse metabolism data can be quickly normalised with a Hoechst fluorescent cell stain.
Read in this AppNote how you can measure cell viability increases and decreases in real-time and avoid time consuming optimisation of a suitable assay time window.
Does your ligand favor arrestin or G-protein signalling? Find out using fluorescent probes and a CLARIOstar® Plus microplate reader!
NanoBRET is used to analyse binding events, signaling pathways and receptor trafficking in live cells and has significantly expanded the range and applications of BRET assays.
Find out about the different types of cell-based assays and why they have become an indispensable tool in a such a broad variety of disciplines in this blog article.
Read here about the various threats associated with mycoplasma contamination and find out about methods to prevent, detect, and eliminate mycoplasma in your cell culture.
Apoptosis is a form of programmed cell death responsible for the removal of damaged or unnecessary cells. Read more about available apoptosis assays here.
Calcium is one of the central players in biology. This blog explains some of the many roles of calcium and discusses the different ways that calcium ions can be measured on microplate readers.
This article highlights the characterisation of hemoglobin and how microplate readers can help to monitor its state or condition based on variations in its absorbance spectrum.
Römer, A;Rawat, D;Linn, T;Petry, SF; [2022]
Biology methods & protocols 7(1) bpab023
Holman, BWB;Fowler, SM;Refshauge, G;Hayes, RC;Newell, MT;Clayton, EH;Bailes, KL;Hopkins, DL; [2022]
Veterinary and animal science 15 100230
da Silva, RA;Xue, R;de Torresi, SIC;Cartmell, S; [2022]
Biointerphases 17(1) 011001
Martín-Martínez, A;Sánchez-Marzo, N;Martínez-Casanova, D;Abarquero-Cerezo, M;Herranz-López, M;Barrajón-Catalán, E;Matabuena-Yzaguirre, M; [2022]
Journal of cosmetic dermatology
Ommati, MM;Li, H;Jamshidzadeh, A;Khoshghadam, F;Retana-Márquez, S;Lu, Y;Farshad, O;Nategh Ahmadi, MH;Gholami, A;Heidari, R; [2022]
Naunyn-Schmiedeberg's archives of pharmacology 395(2) 247-265
Sharma, KS;Dubey, AK;Kumar, C;Phadnis, PP;Sudarsan, V;Vatsa, RK; [2022]
ACS applied bio materials
Interested in monitoring redox changes with roGFP in real-time cell-based assays? Find out here how the CLARIOstar can support you in this.
More measurement points = more information?! This question is investigated in this webinar using the example of cells continuously expressing a synthetic luciferase.
Optimal cell incubation and continuous data collection throughout 72h real-time cell-based assays? No problem with the CLARIOstar Plus and the Atmospheric Control unit!
How much incubator can you find in your reader? The example of endothelial cells shows how important it is to set physiological conditions in cell culture and how these may vary indicidually.
See here, how small measurement intervals on the CLARIOstar Plus enable real-time assessment of GPCR-related Calcium influx in living cells.
Zaida Gloria talks about why she chose the PHERAstar FSX and how this plate reader makes her job easier.
Alfredo Castello-Palomares from Oxford University talks about identifying and characterizing cellular RNA-binding proteins required for viruses to spread.
Ryan McGarrigle from Agilent develops fluorescence-based microplate assays to measure metabolism in different cell types.
Peter McCormick from the William Harvey Research Institute, Queen Mary University London talks about his work and how he can use the CLARIOstar.
Georgios Kosmidis from Ncardia talks about how the CLARIOstar is used in the lab for DNA damage, mitochondrial content and ROS analysis.
Prince Saforo Amponsah from TU Kaiserslautern talks about how redox species (ROS) are generated and regulated and how the CLARIOstar can help.
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