
Cells not only receive extracellular cues, they also translate them into intracellular reactions. Extracellular signaling molecules comprise growth factors, hormones, ions, neurotransmitters, or cytokines.
Cellular reaction time covers from a few seconds to several hours. Accordingly, responses must either be triggered and measured instantly, or they need to be observed in living cells over a longer period of time. BMG LABTECH microplate readers provide injectors and a high sampling rate if fast reactions need to be captured. Further, the Atmospheric Control Unit regulates CO2 and O2 levels inside the reading chamber to provide a feel-good atmosphere for long-term cell experiments.
Signaling events rarely come alone, they take place simultaneously or in concert. In order to clearly characterize signaling events, BMG LABTECH devices offer the possibility of reading several assays in just one well.
Search our resources section for information about specific applications, literature citations, videos, blog articles and many other publications. Many of the resources provided are associated with current and previous instrument models and versions.
Read in this AppNote, how cytokines can be measured and quantified with extraordinary sensitivity, using THUNDER™ Biomarker Assays in combination with the PHERAstar FSX.
Read here how optimised read conditions on the PHERAstar FSX elevate sensitivity and improve the suitability of miniaturised TRF immunoassays for high-throughput screening.
Does your ligand favor arrestin or G-protein signalling? Find out using fluorescent probes and a CLARIOstar® Plus microplate reader!
Read here how you can detect exosome markers with a sensitive europium-based time-resolved immunofluorescence assay on the PHERAstar FSX.
Interested in fast kinetic interaction studies? Read here how the PHERAstar® simultaneously detects luciferase and fluorophore to study the interaction of VEGFR and VEGF with NanoBRET™.
Examining just one second messenger is not enough for you? You can have more! Read how to simultaneously study several second messengers of GPCRs in real-time in living cells.
NanoBRET is used to analyse binding events, signaling pathways and receptor trafficking in live cells and has significantly expanded the range and applications of BRET assays.
Calcium is one of the central players in biology. This blog explains some of the many roles of calcium and discusses the different ways that calcium ions can be measured on microplate readers.
Want to analyse complex cellular signalling events with minimal effort? Multiplexing luciferase assays enable multiple, highly quantitative measurements in a single experiment.
This article highlights how quickly microplate readers read second messenger signalling and how this helps to improve data quality.
Find out how the Dual-Luciferase reporter assay can be utilised to study the influence of the interferon system in virology.
What are G-protein coupled receptors (GPCRs) and how can microplate readers help in their research? Read more in this article.
Gorsek Sparovec, T;Markert, UR;Reif, P;Schoell, W;Moser, G;Feichtinger, J;Mihalic, ZN;Kargl, J;Gargett, CE;Gold, D; [2022]
Stem cell research 60 102671
Finlay, DB;Nguyen, T;Gamage, TF;Chen, S;Barrus, DG;Patel, PR;Thomas, BF;Wiley, JL;Zhang, Y;Glass, M; [2022]
Pharmacology research & perspectives 10(1) e00901
Sobral, MMC;Gonçalves, T;Martins, ZE;Bäuerl, C;Cortés-Macías, E;Collado, MC;Ferreira, IMPLVO; [2022]
Toxins 14(1)
Mohseninia, A;Dehghani, P;Bargahi, A;Rad-Malekshahi, M;Rahimikian, R;Movahed, A;Reza Farzaneh, M;Mohammadi, M; [2022]
International immunopharmacology 104 108522
Törnell, A;Grauers Wiktorin, H;Ringlander, J;Arabpour, M;Nilsson, MR;Nilsson, S;Kiffin, R;Lindh, M;Lagging, M;Hellstrand, K;Martner, A; [2022]
The Journal of infectious diseases
Chen, XX;Khyeam, S;Zhang, ZJ;Zhang, KY; [2022]
Phytomedicine : international journal of phytotherapy and phytopharmacology 97 153923
TRUPATH is a BRET-based platform that enables to monitor discrete GPCR-G protein coupling and can facilitate the discovery of drugs biased towards desired G protein effectors.
Interested in studying functional selectivity for different activation pathways of GPCR agonists? In the same attempt? In real time? Have a look at this approach by Montana Molecular.
Get inspired how to use NanoBRET to monitor G-protein binding, receptor internalisation and receptor heteromerisation.
See here, how small measurement intervals on the CLARIOstar Plus enable real-time assessment of GPCR-related Calcium influx in living cells.
Peter McCormick from the William Harvey Research Institute, Queen Mary University London talks about his work and how he can use the CLARIOstar.
Dmitry Veprintsev & David Sykes talk about GPCR Binding and Signalling Studies and how the PHERAstar FSX can help them.
Maria Arruda from the University of Nottingham tests natural products from the Brazilian biodiversity for drug discovery purposes.
Laura Kilpatrick, Chloe Peach & Carl White from University of Nottingham talk about their work with the receptors VEGFR2 and CXCR4.
Timo Strünker from the University of Münster explains how BMG LABTECH Readers can help at studying the process of fertilisation.
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