
Oxygen Dissociation Assay (ODA): spectrophotometric based screening platform for hemoglobin-O2 affinity modifiers
Oxygen dissociation assay (ODA) in a microplate: How to find modifiers of hemoglobin-oxygen affinity in high throughput?
Cardiovascular diseases are the number one cause of death globally and includes conditions such as arrhythmias, coronary heart disease (CHD), heart attack, high blood pressure, congenital heart defects, vascular dementia, and stroke. CHD is a complex condition and there are many pathways and processes involved in the pathogenesis of the disease, understanding the physiology of ischemia and reperfusion injury is one of the key area of clinical interest but it has been difficult to initiate, maintain and reverse acute hypoxic environments in in-vitro studies. BMG LABTECH’s CLARIOstar Plus reader with Atmospheric Control Unit (ACU) allow oxygen levels to be rapidly and precisely controlled to mimic normal in vivo conditions, ischemia or reperfusion events. Explore the content below to see how we can support ischemia-reperfusion research and cardiovascular research including investigation on what effects oxygen deprivation and reperfusion injury can have on cells, elucidating the role of various cellular processes and identifying potential drug targets to minimize the effects of patients suffering with CHD.
Search our resources section for information about specific applications, literature citations, videos, blog articles and many other publications. Many of the resources provided are associated with current and previous instrument models and versions.
Oxygen dissociation assay (ODA) in a microplate: How to find modifiers of hemoglobin-oxygen affinity in high throughput?
Find out how the oxygen ramping function of the CLARIOstar® microplate reader with Atmospheric Control Unit enables ischemia-reperfusion studies.
In order to investigate Ca2+ transients in vitro you need a reader capable of fast data acquisition. Check out how the CLARIOstar was used for the measurement of real-time calcium flux.
The Predictor hERG Fluorescence Polarization Assay Kit is a homogenous assay to test the capability of new drug candidates to block of the hERG channel. Read more here.
This article talks about ischemia reperfusion research and clarifies how you can mimic physiological and pathological gas conditions with the ACU accessory at the CLARIOstar plate reader.
This article highlights the benefits of microplate readers for mimicking the in vivo environment of coronary artery disease to study pathological changes and detect potential drugs.
Wood, AR;Foliaki, ST;Groveman, BR;Walters, RO;Williams, K;Yuan, J;Zou, WQ;Haigh, CL; [2022]
Scientific reports 12(1) 15788
Hong, JK;Ruhoff, AM;Mathur, K;Neto, C;Waterhouse, A; [2022]
Advanced healthcare materials e2201360
Varjú, I;Tóth, E;Farkas, ÁZ;Farkas, VJ;Komorowicz, E;Feller, T;Kiss, B;Kellermayer, MZ;Szabó, L;Wacha, A;Bóta, A;Longstaff, C;Kolev, K; [2022]
Journal of thrombosis and haemostasis : JTH
Tran, T;Lavillegrand, JR;Lereverend, C;Esposito, B;Cartier, L;Montabord, M;Tran-Rajau, J;Diedisheim, M;Gruel, N;Ouguerram, K;Paolini, L;Lenoir, O;Pinteaux, E;Brabencova, E;Tanchot, C;Urquia, P;Lehmann-Che, J;Le Naour, R;Merrouche, Y;Stockmann, C;Mallat, Z;Tedgui, A;Ait-Oufella, H;Tartour, E;Potteaux, S; [2022]
Nature communications 13(1) 5399
Kadir, RRA;Alwjwaj, M;Bayraktutan, U; [2022]
Metabolic brain disease 37(6) 1815-1827
Amico, G;Hemphill, WO;Severino, M;Moratti, C;Pascarella, R;Bertamino, M;Napoli, F;Volpi, S;Rosamilia, F;Signa, S;Perrino, F;Zedde, M;Ceccherini, I;On Behalf Of The Gaslini Stroke Study Group, ; [2022]
Genes 13(7)
The CLARIOstar offers gas ramping – an inevitable feature for the study of ischemia reperfusion as it allows to initiate, maintain, and reverse hypoxic insults and evaluate at the same time.
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