LUMIstar Omega

전용 마이크로 플레이트 루미넌스

LUMIstar^® Omega는 BMG LABTECH의 강력한 발광 마이크로 플레이트 리더로 매우 섬세한 플래시 및 글로우 측정 판독 값을 제공합니다. 이 DLReady 인증 마이크로 플레이트 리더는 BRET 어플리케이션을위한 Simultaneous Dual Emission을 장착 할 수 있으며 멀티 모드 리더로 업그레이드 할 수 있습니다.

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감지 모드

Absorbance
Fluorescence intensity (incl. FRET)
Luminescence (incl. BRET)
Fluorescence polarization
AlphaScreen
Nephelometry
Time-resolved fluorescence
TR-FRET

제품 세부 정보 리뷰 애플리케이션 노트 인용문 기술 사양 부속품 리더 비교

LUMIstar Omega

LUMIstar Omega는 BRET를 포함하여 루미넌스 측정 전용 판독기입니다. 그것은 종점 및 운동 분석에서 모든 발광 응용 프로그램의 매우 민감한 판독 값을 제공합니다. 또한 내장 된 필터 휠과 선택 사양 인 동시 이중 방출 감지 기능을 통해 다중 파장 감지 및 BRET 어플리케이션을 구현할 수 있습니다.

풍모

플래시, 글로우 및 BRET 분석
384-well까지 측정 가능
상단 및 하단 읽기
45 또는 65 ° C까지의 인큐베이션
동시 이중 방출
웰 당 최대 900 데이터 포인트로 잘 스캐닝
측정 지점에서 주입

마이크로플레이트 리더 기술

Gas Vent

BMG LABTECH offers two forms of gas control on its microplate readers. The Atmospheric Control Unit (ACU) for the CLARIOstar is a unique microprocessor-controlled unit that independently regulates both CO2 and O2 inside the microplate reader. Alternatively, the Gas Vent is a positive pressure gas purge through which the internal atmosphere of the microplate reader can be replaced.

Incubation and shaking

Shaking and incubation allow users to mix the well content and easily run measurements at elevated temperatures. The incubator controls the air temperature in the measurement chamber from room temperature up to 45°C, 60°C or 65°C. BMG LABTECH microplate readers are equipped with orbital, double-orbital or linear shaking with definable intensity, duration and direction of motion. These features are available on all BMG LABTECH plate readers.

Path length correction

Absorbance measurements such as DNA quantification or protein determination detected in a microplate reader are frequently compared and/or normalized to results from a 1 cm path length cuvette. All BMG LABTECH microplate readers have the ability to perform a 1 cm path length correction thanks to an internal fitting algorithm. Using this correction, data normalization to 1 cm path is given with only one mouse click.

Reagent injectors

Many of today's most popular assays such as enzyme kinetics, calcium flux, and dual luciferase require the ability to monitor a signal during and after the addition of a reagent. All BMG LABTECH readers, with the exception of the SPECTROstar Nano, can be equipped with up to two reagent injectors. Injectors enable reagent delivery to any plate format from 6 to 384 wells.

Simultaneous Dual Emission

Simultaneous Dual Emission (SDE) detection allows the measurement of two emitted wavelengths at the same time. It reduces plate read times and corrects for any signal variation due to differences in well volumes, concentrations, or fluctuations in excitation energy. SDE is the ideal tool for double-emission assays such as FRET, TR-FRET, fluorescence polarization and BRET, since the sample only has to be read once.

UV/vis spectrometer

BMG LABTECH was the first and is the only microplate reader manufacturer to equip its instruments with a UV/vis spectrometer for absorbance measurements. Similar to a monochromator, but much faster, the spectrometer will capture full-absorbance spectra from 220 to 1000 nm at a resolution of 1 nm in less than one second/well. Alternatively, up to eight discrete wavelengths can be collected at the same time.

Well-scanning

For non-homogeneous samples such as for cell-based assays, where the sample distribution is uneven across the well, a single reading in the centre of the well is inadequate. To overcome this problem, all BMG LABTECH readers come with an advanced Well Scanning feature that can take multiple measurements in each well with a resolution of up to 900 points/well. Alternatively, in spiral or orbital averaging mode, the reader takes several measurements for each well on a defined spiral or orbit and calculates an average.

Xenon Flash lamps

Microplate readers require a light source to provide the incident of light directed at the sample.

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