Le LUMIstar^® Omega est le lecteur de microplaques performant en luminescence dédié de BMG LABTECH, qui fournit des lectures très sensibles et précises en flash et en glow. Ce lecteur de microplaques certifié DLReady peut également être équipé d'une double émission simultanée pour les applications BRET et peut être mis à niveau vers un lecteur multi-mode.

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Detection Modes

LUMIstar Omega

LUMIstar Omega est un lecteur dédié aux mesures de luminescence, y compris le BRET. Il fournit des lectures très sensibles pour toutes les applications de luminescence que ce soit les essais en point final ou les tests cinétiques. De plus, une roue de filtre intégrée et la détection de double émission simultanée optionnelle permettent une détection de longueur d'onde multiple et des applications BRET possibles.

Two certifications of BMG LABTECH plate readers: DLR, MycoAlert (from left to right)

Caractéristiques

Tests Flash, Glow et BRET
Mesures des plaques allant jusqu'à 384 puits
Lecture par-dessus et par-dessous
Incubation jusqu'à 45 ou 65 °C
Double Emission simultanée
Lecture multipoints avec jusqu'à 900 points de données par puits
Injection au point de mesure

Témoignage client

Customer quotation with the LUMIstar Omega above

Incubation and shaking

Shaking and incubation allow users to mix the well content and easily run measurements at elevated temperatures. The incubator controls the air temperature in the measurement chamber from room temperature up to 45°C, 60°C or 65°C. BMG LABTECH microplate readers are equipped with orbital, double-orbital or linear shaking with definable intensity, duration and direction of motion. These features are available on all BMG LABTECH plate readers.

Open PHERAstar showing pumps for reagent injection

Reagent injectors

Many of today's most popular assays such as enzyme kinetics, calcium flux, and dual luciferase require the ability to monitor a signal during and after the addition of a reagent. All BMG LABTECH readers, with the exception of the SPECTROstar Nano, can be equipped with up to two reagent injectors. Injectors enable reagent delivery to any plate format from 6 to 384 wells.

Automation

Automation is a key feature in high-throughput screening. BMG LABTECH microplate readers have been successfully integrated into various robotic systems. Small footprint, multiple robotic software interfaces and an automation-friendly plate carrier guarantee an easy integration into all leading robotic platforms. All plate readers can be controlled through ActiveX or DDE interfaces. Moreover, the ASCII data output is the most versatile in the industry and works with most LIMS and automation platforms.

Gas Vent

BMG LABTECH offers two forms of gas control on its microplate readers. The Atmospheric Control Unit (ACU) for the CLARIOstar is a unique microprocessor-controlled unit that independently regulates both CO₂ and O₂ inside the microplate reader. Alternatively, the Gas Vent is a positive pressure gas purge through which the internal atmosphere of the microplate reader can be replaced.

Path length correction

Absorbance measurements such as DNA quantification or protein determination detected in a microplate reader are frequently compared and/or normalized to results from a 1 cm path length cuvette. All BMG LABTECH microplate readers have the ability to perform a 1 cm path length correction thanks to an internal fitting algorithm. Using this correction, data normalization to 1 cm path is given with only one mouse click.

Schematic of a well where the way of the spiral scanning is indicated

Well-scanning

For non-homogeneous samples such as for cell-based assays, where the sample distribution is uneven across the well, a single reading in the centre of the well is inadequate. To overcome this problem, all BMG LABTECH readers come with an advanced Well Scanning feature that can take multiple measurements in each well with a resolution of up to 900 points/well. Alternatively, in spiral or orbital averaging mode, the reader takes several measurements for each well on a defined spiral or orbit and calculates an average.

Schematic description of a Dual Emission module of the PHERAstar FSX microplate reader from BMG LABTECH

Simultaneous Dual Emission

Simultaneous Dual Emission (SDE) detection allows the measurement of two emitted wavelengths at the same time. It reduces plate read times and corrects for any signal variation due to differences in well volumes, concentrations, or fluctuations in excitation energy. SDE is the ideal tool for double-emission assays such as FRET, TR-FRET, fluorescence polarization and BRET, since the sample only has to be read once.