DLR assay system

The Dual-Luciferase® Reporter kit from Promega is a popular luminescent reporter assay to study gene transcription and regulation.

Dr Martin Mangold Dr Martin Mangold (7)

The Dual-Luciferase® Reporter (DLR™) assay system from Promega Corp. provides a luminescent reporter assay to study gene transcription and regulation. The activities of two luciferases are measured sequentially from a single sample reporting the transcription of the gene of interest and a transfection control.

Fig. 1: Explanation of DLR assay system

Both assays can be completed in about 4 seconds using a luminometer with reagent auto-injectors, such as in the Omega seriesVANTAstar™CLARIOstar®, and PHERAstar® FSX microplate readers. In the DLR™ assay system, both reporters yield linear assays with attomole sensitivities and no endogenous activity in the experimental host cells. Furthermore, the integrated format of the DLR™ assay provides rapid quantitation of both reporters either in transfected cells or in cell-free transcription/translation reactions.

Fig. 2: Dual Luciferase Reaction - Luciferase Assay Reagent II (LAR II) is injected in the first step and the Firefly reaction is started. Stop and Glo® buffer is injected in the second step, which quenches the Firefly reaction and initiates the Renilla reaction.

 

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