LUMIstar Omega

Luminómetro de microplaca dedicado

El LUMIstar Omega es el potente lector de luminiscencia dedicado para microplacas, proporciona alta sensibilidad en lecturas flash y glow. Este lector de microplacas certificado DLReady puede ser equipado con Emisión Simultanea Dual para aplicaciones de BRET y puede ampliarse ulteriormente a lector multimodo.

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Modos de detección

Absorbance
Fluorescence intensity (incl. FRET)
Luminescence (incl. BRET)
Fluorescence polarization
AlphaScreen
Nephelometry
Time-resolved fluorescence
TR-FRET

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LUMIstar Omega

El LUMIstar Omega es un lector dedicado para medidas de luminiscencia, ibncluyendo BRET. El LUMIstar Omega ofrece alta sensibilidad en todas las aplicaciones d eluminiscencia en ensayos cinéticos o a punto final. Además, la rueda de filtros que incluye y el sistema opcional de detección de Emisión Simultanea Dual hacen posible aplicaciones de detección de múltiples longitudes de onda y BRET.

Caracteristicas

Ensayos Flash, Glow y BRET
Medida de placas de hasta 384 pocillos.
Lectura por arriba y por debajo
Incubación hasta 45 o 65 ° C
Emisión Simultanea Dual
Escaneado de pocillo con hasta 900 puntos de datos por pocillo
Inyección en el punto de medición

Tecnologías de lectores de microplacas

Gas Vent

BMG LABTECH offers two forms of gas control on its microplate readers. The Atmospheric Control Unit (ACU) for the CLARIOstar is a unique microprocessor-controlled unit that independently regulates both CO2 and O2 inside the microplate reader. Alternatively, the Gas Vent is a positive pressure gas purge through which the internal atmosphere of the microplate reader can be replaced.

Incubation and shaking

Shaking and incubation allow users to mix the well content and easily run measurements at elevated temperatures. The incubator controls the air temperature in the measurement chamber from room temperature up to 45°C, 60°C or 65°C. BMG LABTECH microplate readers are equipped with orbital, double-orbital or linear shaking with definable intensity, duration and direction of motion. These features are available on all BMG LABTECH plate readers.

Path length correction

Absorbance measurements such as DNA quantification or protein determination detected in a microplate reader are frequently compared and/or normalized to results from a 1 cm path length cuvette. All BMG LABTECH microplate readers have the ability to perform a 1 cm path length correction thanks to an internal fitting algorithm. Using this correction, data normalization to 1 cm path is given with only one mouse click.

Reagent injectors

Many of today's most popular assays such as enzyme kinetics, calcium flux, and dual luciferase require the ability to monitor a signal during and after the addition of a reagent. All BMG LABTECH readers, with the exception of the SPECTROstar Nano, can be equipped with up to two reagent injectors. Injectors enable reagent delivery to any plate format from 6 to 384 wells.

Simultaneous Dual Emission

Simultaneous Dual Emission (SDE) detection allows the measurement of two emitted wavelengths at the same time. It reduces plate read times and corrects for any signal variation due to differences in well volumes, concentrations, or fluctuations in excitation energy. SDE is the ideal tool for double-emission assays such as FRET, TR-FRET, fluorescence polarization and BRET, since the sample only has to be read once.

UV/vis spectrometer

BMG LABTECH was the first and is the only microplate reader manufacturer to equip its instruments with a UV/vis spectrometer for absorbance measurements. Similar to a monochromator, but much faster, the spectrometer will capture full-absorbance spectra from 220 to 1000 nm at a resolution of 1 nm in less than one second/well. Alternatively, up to eight discrete wavelengths can be collected at the same time.

Well-scanning

For non-homogeneous samples such as for cell-based assays, where the sample distribution is uneven across the well, a single reading in the centre of the well is inadequate. To overcome this problem, all BMG LABTECH readers come with an advanced Well Scanning feature that can take multiple measurements in each well with a resolution of up to 900 points/well. Alternatively, in spiral or orbital averaging mode, the reader takes several measurements for each well on a defined spiral or orbit and calculates an average.

Xenon Flash lamps

Microplate readers require a light source to provide the incident of light directed at the sample.

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Hussein, ZS;Hegazy, AK;Mohamed, NH;El-Desouky, MA;Ibrahim, SD;Safwat, G; [2022]
Journal, genetic engineering & 61511166610 20(1) 135

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Placental OLAH Levels Are Altered in Fetal Growth Restriction, Preeclampsia and Models of Placental Dysfunction

de Alwis, N;Beard, S;Binder, NK;Pritchard, N;Kaitu'u-Lino, TJ;Walker, SP;Stock, O;Groom, K;Petersen, S;Henry, A;Said, JM;Seeho, S;Kane, SC;Tong, S;Hui, L;Hannan, NJ; [2022]
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Ver todas las notas de aplicación

Eco-physiological response and genotoxicity induced by crude petroleum oil in the potential phytoremediator Vinca rosea L

Hussein, ZS;Hegazy, AK;Mohamed, NH;El-Desouky, MA;Ibrahim, SD;Safwat, G; [2022]
Journal, genetic engineering & 61511166610 20(1) 135

Read article

Airway smooth muscle thickness and contraction are enhanced by intra-amniotic lipopolysaccharide in an ovine model of premature birth

Savigni, DL;Chang, AY;Sorensen, NL;Papagianis, PC;Ahmadi-Noorbakhsh, S;Pillow, JJ;Noble, PB; [2022]
Journal of applied physiology (Bethesda, Md. : 1985) 133(4) 959-969

Read article

Bacteriophage as a potential therapy to control antibiotic-resistant Pseudomonas aeruginosa infection through topical application onto a full-thickness wound in a rat model

Rezk, N;Abdelsattar, AS;Elzoghby, D;Agwa, MM;Abdelmoteleb, M;Aly, RG;Fayez, MS;Essam, K;Zaki, BM;El-Shibiny, A; [2022]
Journal, genetic engineering & 61511166610 20(1) 133

Read article