LUMIstar Omega

発光測定専用マイクロプレートリーダー

BMG LABTECHのLUMIstar Omega^®は、フラッシュアッセイ・グローアッセイともに高感度に測定できる発光測定専用のマイクロプレートリーダーです。プロメガ社のDLReadyアッセイの承認を受けており、デュアルの検出器を搭載することで二波長同時測定を可能にしたLUMIstar Omegaは、BRETアプリケーションにも対応します。また、その他の測定モードを追加してマルチプレートリーダーにアップグレードが可能です。

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検出モード

Absorbance
Fluorescence intensity (incl. FRET)
Luminescence (incl. BRET)
Fluorescence polarization
AlphaScreen
Nephelometry
Time-resolved fluorescence
TR-FRET

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LUMIstar Omega

LUMIstar Omegaは、BRETを含む発光測定の専用リーダーです。エンドポイント・カイネティックすべての発光測定に高感度で対応します。さらに、内蔵型フィルターホイールとオプションのデュアル検出部により、複数の波長を検出したいアプリケーションやBRETアッセイに対応します。

特徴

フラッシュアッセイ・グローアッセイ及びBRETアッセイ
384wellプレート対まで対応
上方測定＆下方測定
45℃もしくは65℃までの温度制御
2波長同時測定
ウェル毎に最大900ポイントでウェルスキャニング
試薬注入と検出を同時に行う高精度インジェクター（2chまで)

マイクロプレートリーダーテクノロジー

Gas Vent

BMG LABTECH offers two forms of gas control on its microplate readers. The Atmospheric Control Unit (ACU) for the CLARIOstar is a unique microprocessor-controlled unit that independently regulates both CO2 and O2 inside the microplate reader. Alternatively, the Gas Vent is a positive pressure gas purge through which the internal atmosphere of the microplate reader can be replaced.

Incubation and shaking

Shaking and incubation allow users to mix the well content and easily run measurements at elevated temperatures. The incubator controls the air temperature in the measurement chamber from room temperature up to 45°C, 60°C or 65°C. BMG LABTECH microplate readers are equipped with orbital, double-orbital or linear shaking with definable intensity, duration and direction of motion. These features are available on all BMG LABTECH plate readers.

Path length correction

Absorbance measurements such as DNA quantification or protein determination detected in a microplate reader are frequently compared and/or normalized to results from a 1 cm path length cuvette. All BMG LABTECH microplate readers have the ability to perform a 1 cm path length correction thanks to an internal fitting algorithm. Using this correction, data normalization to 1 cm path is given with only one mouse click.

Reagent injectors

Many of today's most popular assays such as enzyme kinetics, calcium flux, and dual luciferase require the ability to monitor a signal during and after the addition of a reagent. All BMG LABTECH readers, with the exception of the SPECTROstar Nano, can be equipped with up to two reagent injectors. Injectors enable reagent delivery to any plate format from 6 to 384 wells.

Simultaneous Dual Emission

Simultaneous Dual Emission (SDE) detection allows the measurement of two emitted wavelengths at the same time. It reduces plate read times and corrects for any signal variation due to differences in well volumes, concentrations, or fluctuations in excitation energy. SDE is the ideal tool for double-emission assays such as FRET, TR-FRET, fluorescence polarization and BRET, since the sample only has to be read once.

UV/vis spectrometer

BMG LABTECH was the first and is the only microplate reader manufacturer to equip its instruments with a UV/vis spectrometer for absorbance measurements. Similar to a monochromator, but much faster, the spectrometer will capture full-absorbance spectra from 220 to 1000 nm at a resolution of 1 nm in less than one second/well. Alternatively, up to eight discrete wavelengths can be collected at the same time.

Well-scanning

For non-homogeneous samples such as for cell-based assays, where the sample distribution is uneven across the well, a single reading in the centre of the well is inadequate. To overcome this problem, all BMG LABTECH readers come with an advanced Well Scanning feature that can take multiple measurements in each well with a resolution of up to 900 points/well. Alternatively, in spiral or orbital averaging mode, the reader takes several measurements for each well on a defined spiral or orbit and calculates an average.

Xenon Flash lamps

Microplate readers require a light source to provide the incident of light directed at the sample.

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