FLUOstar Omega

Name: FLUOstar Omega
Brand: BMG LABTECH
Rating: 4.7 (47 reviews)

Filter-basierter multi-mode Reader der Omega Serie

Der FLUOstar^® Omega ist ein multi-mode Microplate Reader mit drei Detektionsmethoden. Der Reader nutzt ein ultraschnelles UV/vis-Spektrometer für Absorption und hochsensible optische Filter für alle weiteren Detektionsmethoden. Der FLUOstar Omega ist der ideale Microplate Reader für Anwendungen im Life-Science-Bereich.

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Detektionsmethoden

Absorbance
Fluorescence intensity (incl. FRET)
Luminescence (incl. BRET)
Fluorescence polarization
AlphaScreen
Nephelometry
Time-resolved fluorescence
TR-FRET

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FLUOstar Omega

Der FLUOstar Omega ist ein vielseitiger multi-mode Microplate Reader mit sechs Detektionsmethoden: Fluoreszenz Intensität (inklusive FRET), Lumineszenz (inklusive BRET), UV/Vis Absorption und zeitaufgelöste Fluoreszenz.

Eigenschaften

Absorptionsmessungen mit höchster Geschwindigkeit
Messungen in bis zu 384-Well Mikroplatten
Top- und Bottom-Reading
Inkubation bis zu 45 °C oder 65 °C
Benutzerdefinierte Schüttelfunktionen
Well-Scanning mit bis zu 900 Messpunkten pro Well
Präzise Injektoren mit simultaner Reagenzinjektion und Messung
Messung kleinster Volumina

Referenzen

Microplate Reader Technologien

Gas Vent

BMG LABTECH offers two forms of gas control on its microplate readers. The Atmospheric Control Unit (ACU) for the CLARIOstar is a unique microprocessor-controlled unit that independently regulates both CO2 and O2 inside the microplate reader. Alternatively, the Gas Vent is a positive pressure gas purge through which the internal atmosphere of the microplate reader can be replaced.

Incubation and shaking

Shaking and incubation allow users to mix the well content and easily run measurements at elevated temperatures. The incubator controls the air temperature in the measurement chamber from room temperature up to 45°C, 60°C or 65°C. BMG LABTECH microplate readers are equipped with orbital, double-orbital or linear shaking with definable intensity, duration and direction of motion. These features are available on all BMG LABTECH plate readers.

Path length correction

Absorbance measurements such as DNA quantification or protein determination detected in a microplate reader are frequently compared and/or normalized to results from a 1 cm path length cuvette. All BMG LABTECH microplate readers have the ability to perform a 1 cm path length correction thanks to an internal fitting algorithm. Using this correction, data normalization to 1 cm path is given with only one mouse click.

Reagent injectors

Many of today's most popular assays such as enzyme kinetics, calcium flux, and dual luciferase require the ability to monitor a signal during and after the addition of a reagent. All BMG LABTECH readers, with the exception of the SPECTROstar Nano, can be equipped with up to two reagent injectors. Injectors enable reagent delivery to any plate format from 6 to 384 wells.

UV/vis spectrometer

BMG LABTECH was the first and is the only microplate reader manufacturer to equip its instruments with a UV/vis spectrometer for absorbance measurements. Similar to a monochromator, but much faster, the spectrometer will capture full-absorbance spectra from 220 to 1000 nm at a resolution of 1 nm in less than one second/well. Alternatively, up to eight discrete wavelengths can be collected at the same time.

Well-scanning

For non-homogeneous samples such as for cell-based assays, where the sample distribution is uneven across the well, a single reading in the centre of the well is inadequate. To overcome this problem, all BMG LABTECH readers come with an advanced Well Scanning feature that can take multiple measurements in each well with a resolution of up to 900 points/well. Alternatively, in spiral or orbital averaging mode, the reader takes several measurements for each well on a defined spiral or orbit and calculates an average.

Xenon Flash lamps

Microplate readers require a light source to provide the incident of light directed at the sample.

Cell-free extract protein synthesis: Rapid single vessel DNA assembly and protein production in microliter scale

Read here, how protein synthesis in cell-free extracts can be used to rapidly screen for protein variants in low-volume single vessel reactions with the CLARIOstar Plus.

Extracellular ATP measurement in real time using living cells

Find out, how to perform real-time measurements of Promega´s extracellular ATP kit with living cells with the CLARIOstar Plus with ACU.

Structure-guided monitoring of the human opioid receptor family using BRET assays

Find out how the TRUPATH platform combined with the PHERAstar FSX can be used to study GPCR interaction and thus be able to advance the development of safer opoid drugs for pain relief.

ROS detection in a cell-based format using the Promega ROS-Glo™ assay

There are many ways to monitor ROS generation. But few are as simple, fast, and sensitive as performing Promega’s ROS-GloTM assay on BMG LABTECH’s microplate readers.

Multiplexing Calcium Flux and Metabolic Effect Measurements in iPSC Cardiomyocytes

IPSC cardiomyocytes enable to study drug-induced contractions with high physiological relevance. Read here how the CLARIOstar Plus enables multiplexing also in live cell assays.

Measuring protein ligand binding with an endogenous HiBiT CETSA test system

Do you want to perform interaction studies without having to perform protein purification? Read here, how HiBiT CETSA can be used to study PROTAC-target interaction in an endogenous test system.

Alle Application Notes anzeigen

Novel N-methylsulfonyl-indole derivatives: biological activity and COX-2/5-LOX inhibitory effect with improved gastro protective profile and reduced cardio vascular risks

Philoppes, JN;Abdelgawad, MA;Abourehab, MAS;Sebak, M;A Darwish, M;Lamie, PF; [2023]
Journal of enzyme inhibition and medicinal chemistry 38(1) 246-266

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Simple, Reliable Protocol for High-Yield Solubilization of Seedless Amyloid-β Monomer

Taylor, AIP;Davis, PJ;Aubrey, LD;White, JBR;Parton, ZN;Staniforth, RA; [2023]
ACS chemical 61511166612 14(1) 53-71

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Perphenazine-Macrocycle Conjugates Rapidly Sequester the Aβ42 Monomer and Prevent Formation of Toxic Oligomers and Amyloid

Ball, SR;Adamson, JSP;Sullivan, MA;Zimmermann, MR;Lo, V;Sanz-Hernandez, M;Jiang, X;Kwan, AH;McKenzie, ADJ;Werry, EL;Knowles, TPJ;Kassiou, M;Meisl, G;Todd, MH;Rutledge, PJ;Sunde, M; [2023]
ACS chemical 61511166612 14(1) 87-98

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Endoplasmic reticulum expansion throughout the differentiation of teleost B cells to plasmablasts

Morel, E;Herranz-Jusdado, JG;Simón, R;Abós, B;Perdiguero, P;Martín-Martín, A;Andrés, G;Muñoz-Atienza, E;Guerra Rodriguez, M;Díaz-Rosales, P;Tafalla, C; [2023]
iScience 26(1) 105854

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Quaternized chitosan/chitosan nanofibrous mats: An approach toward bioactive materials for tissue engineering and regenerative medicine

Andreica, BI;Anisiei, A;Rosca, I;Sandu, AI;Pasca, AS;Tartau, LM;Marin, L; [2023]
Carbohydrate polymers 302 120431

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Vimentin epigenetic deregulation in Bladder Cancer associates with acquisition of invasive and metastatic phenotype through epithelial-to-mesenchymal transition

Monteiro-Reis, S;Miranda-Gonçalves, V;Guimarães-Teixeira, C;Martins-Lima, C;Lobo, J;Montezuma, D;Dias, PC;Neyret-Kahn, H;Bernard-Pierrot, I;Henrique, R;Jerónimo, C; [2023]
International journal of biological sciences 19(1) 1-12

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