Luminescence plate reader
Luminescence detection can be performed on the PHERAstar® FSX, CLARIOstar®, and the LUMIstar®, FLUOstar®, and POLARstar® Omega plate readers. All of these luminescence plate readers are certified for Dual-Luciferase Reporter assays (DLReady®) and utilize luminescence-optimized low-noise PMT (photomultiplier tube) detectors which provide a dynamic range of up to nine decades, depending on the reader. All readers can be equipped with high-precision reagent injectors.
Luminescence detection is the measurement of the light emitted as a result of a chemical or biochemical reaction induced by an enzyme on its substrate; no excitation energy is required. According to its kinetic, luminescence can be divided in flash and glow reactions. Flash luminescence gives off a very bright signal for a very short amount of time, usually seconds. Glow luminescence can last for several minutes, but usually emits a less intense signal. BRET (Bioluminescence Resonance Energy Transfer) is a dual reporter luminescence detection assay based upon resonance energy transfer between a bioluminescent donor and a fluorescent acceptor. As the donor emits photons by bioluminescence, fluorescence excitation is dispensable.
Our luminescence plate readers
The following plate readers can be configurated to perform luminescence measurements. Choose between PHERAstar FSX - the high-throughput screening reader for fast read times, CLARIOstar with LVF monochromators™ for highest flexibility and Omega Series - our upgradable microplate readers for luminescence or further dectection modes.
The following are examples of luminescence measurements made on BMG LABTECH microplate readers:
- AN 300: Verifying SPARCL performance on the CLARIOstar equipped for reading at time of injection
- AN 271: Dual Luciferase Reporter (DLR) assay certification
- AN 266: Promega's multiplexed cell viability and apoptosis assays
See a complete list of luminescence application notes.