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Colorimetric and turbidimetric analysis of endotoxins using the absorbance detection mode
Torn between a colorimetric or turbidimetric LAL assay? Whichever path you take, BMG LABTECH’s absorbance readers deliver dependable results every time.
PHERAstar FSX
강력하고 가장 민감한 HTS 플레이트 리더기
PHERAstar® FSX는 고감도 스크리닝을위한 최신의 가장 민감한 멀티 모드 리더이며 빠른 판독 시간으로 감도를 결합합니다. 고유한 광학 모듈 시스템, 동시 이중 방출, 향상된 다이내믹 레인지 기술, UV/vis 분광계, AAS 시스템 및 전용 알파 스크린® 및 TRF 레이저 장착으로 인해, PHERAstar FSX는 3456 웰 마이크로플레이트에서도 쉽고 빠르게 측정할 수 있습니다.
평가
A must equipment for any lab!
PheraStar is really easy to use, to assess and define protocol in order to get reproducible...
4.8/5.0 (81)
PHERAstar FSX
더 이상 필터 및 다이크로익 설치에 대한 걱정을하지 않아도됩니다. PHERAstar FSX는 모든 특수 용도 필터, 거울, 다이크로익 또는 편광판을 포함하고 있으며 플레이트 판독기에서 자동으로 인식 할 수있는 분석 용이 한 광학 최적화 모듈과 함께 제공됩니다. 또한 PHERAstar FSX에는 관련 검출 모드에 맞게 자동으로 선택된 4 개의 정합 및 분석 최적화 광전자 배증 관 (PMT)이 장착되어 있습니다. Simultaneous Dual Emission 덕분에 동시에 두 개의 방출 파장을 가진 분석을 측정 할 수 있습니다.
향상된 다이내믹 레인지와 자동 초점 기술을 활용하여, 수동 설정이 필요없고, 측정 값 최적화 용이
HTS 자동화를 위해 PHERAstar FSX는 자동화 시스템과 쉽게 연동할 수 있고, 로봇과 연계하여 사용할 수 있으며, 여러 사용자가 제어할 수 있습니다.
PHERAstar FSX는 연구용으로만 사용됩니다.
주요 기능
- 형광 강도와 편광에서 가장 민감한 판독기
- 5 가지 분석 전용 탐지기가있는 유일한 플레이트 판독기
- 알파 기술을 포함한 동시 이중 방출
- 향상된 다이내믹 레인지 및 자동 초점
- 전용 AlphaScreen® / AlphaLISA® / AlphaPlex™ 레이저
- 차세대 TRF 레이저
- 상단 및 하단 초점 높이 조정
- 동시 시약 주입 및 검출 기능이있는 고정밀 인젝터
- 보다 향상된 분석을 위한 안정성 시스템
신뢰와 사랑을 받고 있습니다:
PHERAstar FSX
우리의 기술
Measurement of a reconstituted circadian clock using fluorescence polarization
Unlocking Nature’s Timekeepers. Learn how to read the internal clock of organisms and how to decode life’s 24-hour rhythm at the molecular level using a microplate reader.
Multiplexing the production of two G protein-coupled receptor second messengers using spectrally resolved fluorescent dyes
Streamline your workflow and double your insights: multiplexing cellular signal responses of multiple second messengers in a single measurement saves valuable time and conserves reagents.
Detection of bacterial endotoxins using the PYROSTAR™ ES-F/Plate LAL assay
Perform the PYROSTAR ES-F/Plate LAL test your way— BMG LABTECH readers support both turbidimetric and nephelometric evaluation for maximum flexibility.
Determination of Minimum Inhibitory Concentration (MIC) of antibiotics using OD600
Do you want to make your MIC measurements more efficient and automated? Find out here how BMG LABTECH microplate readers can support you in this task.
Stream-lined detection of endotoxin using the ENDOZYME II recombinant factor C assay and Enhanced Dynamic Range on the VANTAstar
Do you want to streamline your endotoxin detection workflow? Check out what the combination of VANTAstar and ENDOZYME II can do for you.
Detection Modes
Fluorescence intensity - incl. FRET
Fluorescence polarization
AlphaScreen®/AlphaLISA®/AlphaPlex™
Luminescence (flash and glow) - incl. BRET
Time-resolved fluorescence
TR-FRET
UV/Vis absorbance spectra
Measurement Modes
Top and bottom reading
Endpoint and kinetic
Sequential multi-excitation
Sequential multi-emission
Simultaneous Dual Emission
Spectral scanning (absorbance)
Real-time ratiometric measurements
Well scanning
Microplate Formats
Up to 3456-well plates, user-definable
LVis Plate with 16 low volume microspots (2 μL)
Microplate Carrier
Robot compatible
Light Sources
High energy xenon flash lamp
Dedicated laser for AlphaScreen®/AlphaLISA®/AlphaPlex™
Dedicated laser for TRF and TR-FRET
Detectors
Two matched pairs of photomultiplier tubes (PMTs), optimized for different detection modes
CCD spectrometer
Wavelength Selection
Optic Modules: Up to six application-specific modules (includes all filters, dichroics, etc. for an application)
For top and bottom reading
UV/vis absorbance spectrometer: Full spectra or 8 distinct wavelengths in < 1 sec/well
Spectral Range
Filters
230 - 750 nm or 230 - 900 nm for FI, FP
230 - 750 nm for LUM
230 - 900 nm for TRF
Spectrometer
220 - 1000 nm for ABS; wavelength precision: ≤ 0.5 nm
Sensitivity
FI
(top)
< 0.15 pM FITC (384sv black, 20 μL)
< 0.5 pM FITC (1536 black, 8 μL)
Dynamic Range: 8 decades
FI
(bottom)
<1.0 pM FITC (384g black, 50 μL)
FP
< 0.5 mP SD at 1 nM FITC (384sv black, 20 μL)
< 1.5 mP SD at 1 nM FITC (1536 black, 8 μL)
TRF with laser.
< 5 fM europium (384, 80 μL)
< 15 fM europium (1536, 8 μL)
HTRF®
(black and white microplates)
Reader Control Kit (Eu) after 18h (384sv, 20 μL)
Delta F > 1100% (High Calibrator)
Delta F > 25% (Low Calibrator)
LUM
< 0.4 pM (< 8 amol/well ATP, 384sv white, 20 μL)
Dynamic Range: 8 decades
AlphaScreen® with laser
< 5 pM (< 100 amol/well P-Tyr-100, 384sv white, 20 μL)
ABS with spectrometer
Selectable spectral resolution: 1, 2, 5, and 10 nm
OD range: 0 to 4 OD; photometric resolution: 0.001 OD
Accuracy: < 1% at 2 OD
Precision: < 0.5% at 1 OD and < 0.8% at 2 OD
Linearity: ≤ 0.8% at 2.0 OD
Read Times
Flying mode (1 flash)
14 s (384)
27 s (1536)
10 flashes
38 s (384)
1 min 52 s (1536)
50 flashes
1 min 29 s (384)
5 min 18 s (1536)
Shaking
Linear, orbital, and double-orbital with user-definable time and speed
Incubation
+5 °C above ambient up to 45 °C
The upper heating plate of the incubation chamber operates at +0.5 °C more than the lower plate. This prevents condensation build-up on the lid or sealer
With Advanced Assay Stability (AAS) system: from 18 - 45°C
Software
Multi-user Reader Control and MARS Data Analysis Software included
FDA 21 CFR Part 11 compliant
Dimensions
Width: 45 cm, depth: 51 cm, height: 47 cm; weight: 49 kg
With AAS system: width: 48 cm, depth: 68 cm, height: 47 cm; weight: 62 kg
Optical Path Guides
Top and bottom: free-air optical light path guided by motor-driven mirrors and dichroics
Z-Adjustment
Automatic focal height adjustment (0.1 mm resolution)
Reagent Injection
Up to 2 built-in reagent injectors
Injection at measurement position (6 to 384-well)
Individual injection volumes for each well (3 to 500 µL, optionally up to 2 mL)
Variable injection speed up to 420 µL/s
Up to four injection events per well
Reagent back flushing
Integrated Barcode Reader
Up to three integrated barcode readers
Power Requirements
- 100 or 115 or 230 V, 50/60 Hz, auto ranging
Power Consumption
- Normal operating conditions: 50 W
- Idle State: 20 W
- AAS-option: + 30 W
- Switched off: 0 W max. 300 VA
Optional Accessories
LVis Plate
Sample Capacity: sixteen separate microdrop wells for 2 µL samples; one standard cuvette position for up to 1 mL samples
Quality Control Internal Standards (optional): four NIST traceable optical density filters (approximate values of 0.1, 0.3, 0.6 and 1.0 OD); one holmium oxide filter for wavelength accuracy
Dimensions: conforms to SBS standards for microplates
THERMOstar
Microplate incubator and shaker
Stacker
Magazines for up to 50 plates - continuous loading feature
Upgrades
Please contact your local representative for upgrades including options such as detection modes, reagent injectors, etc
*
LOD = 3 x SD (20 blanks) / slope (6 pt std curve)
AlphaScreen® P-Tyr-100 assay kit, PerkinElmer, #6760620C
384sv = 384-well small volume microplates
384g = 384-well glass bottom microplates
1536 = 1536-well microplates
The PHERAstar FSX is for research use only.
더 많은 효과를 얻으려면 : PHERAstar FSX 액세서리
THERMOstar
Intelligent microplate incubation
Microplate Stacker
Microplate handling system
LVis Plate
Low-volume measurements
Filters
Filters for a wide range of applications
Evaluation Plate
Functional Microplate Reader Validation