Decay Curve Monitoring

Decay Curve Monitoring (DCM) is a fundamental tool for TRF, TR-FRET, and AlphaScreen® assay development. It enables the collection of fluorophore decay curves for one or two wavelengths with a user-selectable resolution. Assay performance can be improved by altering the measurement delay time or the signal integration time. Areas in the measurement protocol can also be identified and altered to increase the count rate.

DCM is a unique PHERAstar® FSX microplate reader feature.

Fig. 1: The PHERAstar FSX offers decay curve monitoring that allows the signal decay of individual fluorophores to be examined precisely and resolved in microsecond intervals.

While other TR-FRET microplate readers simply give an integration value for the area under the curve, the PHERAstar®FSX provides much more! For TR-FRET assays, DCM takes advantage of a special TRF-dedicated PMT with a direct photon measurement system which allows for microsecond detection.

DCM can be used for TRF, TR-FRET, and AlphaScreen® assays and provides an indispensable tool for assay troubleshooting or for performance fine-tuning.

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