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Colorimetric and turbidimetric analysis of endotoxins using the absorbance detection mode
Torn between a colorimetric or turbidimetric LAL assay? Whichever path you take, BMG LABTECH’s absorbance readers deliver dependable results every time.
FLUOstar Omega
フィルターベースのマルチモードマイクロプレートリーダー
FLUOstar Omegaは、最大3つの測定モードを組み合わせることができるマルチモードマイクロプレートリーダーです。吸光度測定では、超高速UV/Visスペクトロメーター式またはフィルター式を選択できます。その他の測定モードでは高感度フィルターを用いた測定を行います。FLUOstar Omega®は、ライフサイエンス分野のアプリケーションに理想的なプレートリーダーです。
評価
One of the best devices in my lab
We got our Fluostar Omega in 2010. It has been in frequent regular use since then and was...
4.7/5.0 (53)
FLUOstar Omega
FLUOstar Omegaは、汎用性の高いマルチモードマイクロプレートリーダーです。蛍光測定（FRET・時間分解蛍光測定含む）、発光測定（BRET含む）、吸光度測定のうち最大3つの測定モードを組み合わせることができます。
FLUOstar Omegaは研究用途にのみご使用いただけます。
特徴
- 高速スペクトル吸光度測定
- 384ウェルプレート対応
- 上方測定および下方測定に対応
- 45℃または65℃までの温度制御
- 選択性の高い各種シェイキング機能
- ウェルごとに最大900ポイントでのウェルスキャニング
- 試薬注入と検出を同時に行う高精度インジェクター（2chまで）
- 微量測定に対応
信頼され、支持されています：
オプションアクセサリー
- Atmospheric Control Unit: O₂・CO₂ ガス制御モジュール
- THERMOstar: マイクロプレートインキュベーターおよびオービタルシェーカー
- Microplate Stacker: マイクロプレート処理システム
- LVis Plate: 低容量測定および性能テスト用プレート
- Filters: 幅広い用途に対応したフィルター
- Evaluation Plate: 光学機能検証用プレート
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Detection Modes
Fluorescence intensity - incl. FRET
Luminescence (flash and glow) - incl. BRET
UV/Vis absorbance spectra
Measurement Modes
Top and bottom reading
Endpoint and kinetic
Sequential multi excitation
Sequential multi emission
Simultaneous Dual Emission (FI and LUM)
Spectral scanning (absorbance)
Ratiometric measurements
Microplate Formats
6 to 384-well plates, user-definable
LVis Plate with 16 low volume microspots (2 μL)
Microplate Carrier
Robot compatible
Light Sources
High energy xenon flash lamp
Detectors
Low noise photomultiplier tube
CCD spectrometer
Wavelength Selection
Optical filters: ex and em wheels for 8 filters each
UV/vis absorbance spectrometer: full spectra or 8 distinct wavelengths in < 1 sec/well
Spectral Range
Filters
240 - 740 nm or 240 - 900 nm for FI
240 - 740 nm for LUM
Spectrometer
220 - 1000 nm for ABS; wavelength precision: ≤ 0.5 nm
Sensitivity
FI
<0.2 fmol/well FITC.
TRF
< 30 amol/well europium.
LUM
< 20 amol/well ATP.
ABS with spectrometer
Selectable spectral resolution: 1, 2, 5, and 10 nm.
OD range: 0 to 4 OD; photometric resolution: 0.001 OD.
Accuracy: < 1% at 2 OD.
Precision: < 0.5% at 1 OD and < 0.8% at 2 OD.
Linearity: ≤ 0.8% at 2.0 OD.
ABS with filters
OD range: 0 to 4 OD
Reproducibility: ±0.010 OD for 0-2 OD range
Read Times
Flying mode (1 flash)
9 s (96), 16 s (384)
Shaking
Linear, orbital, and double-orbital with user-definable time and speed
Purge Gas Vent
System to inject an atmosphere or to pull a vacuum into the reader
Incubation
+4 °C above ambient up to 45 °C or 65 °C
The upper heating plate of the incubation chamber operates at 0.5 °C more than the lower plate. This prevents condensation build-up on the lid or sealer
Software
Multi-user Reader Control and MARS Data Analysis Software included
FDA 21 CFR Part 11 compliant
Dimensions
Width: 44 cm, depth: 48 cm, height: 30 cm; weight: 28 kg
Optical Filters
Excitation and emission filter wheels for 8 filters each
Optical Path Guides
Top: liquid-filled light guides
Bottom: fiber optics
Reagent Injection
Up to 2 built-in reagent injectors
Injection at measurement position (6 to 384-well)
Individual injection volumes for each well (3 to 500 µL)
Variable injection speed up to 420 µL/s
Up to four injection events per well
Reagent back flushing
Power Requirements
- 100-240 V, 50/60 Hz, max. peak 300 VA
Power Consumption
- Instrument off: 0 W
- Normal operating conditions: 25 W
- Normal operating conditions, incubation set to 37°C: 60 W
Optional Accessories
LVis Plate
Sample capacity: sixteen separate microdrop wells for 2 µL samples; one standard cuvette position for up to 1 mL samples
Quality control internal standards (optional): four NIST traceable optical density filters (approximate values of 0.1, 0.3, 0.6 and 1.0 OD); one holmium oxide filter for wavelength accuracy
Dimensions: conforms to SBS standards for microplates
THERMOstar
Microplate incubator and shaker
Atmospheric Control Unit (ACU)
Actively regulates O2 and CO2 - 0.1-20%
Stacker
Magazines for up to 50 plates - continuous loading feature
Optical Filters
Excitation and emission filters
Upgrades
Please contact your local representative for upgrades including options such as detection modes, reagent injectors, etc
*
LOD = 3 x SD (20 blanks) / slope (6 pt std curve)
Microplates: white for LUM; black for FI; clear for ABS
96 = 96-well microplates
384sv = 384-well small volume microplates
384g = 384-well glass bottom microplates
The FLUOstar Omega is for research use only.
高感度対応アクセサリー FLUOstar Omega
THERMOstar
Intelligent microplate incubation
Microplate Stacker
Microplate handling system
LVis Plate
Low-volume measurements
Filters
Filters for a wide range of applications
Evaluation Plate
Functional Microplate Reader Validation
Atmospheric Control Unit
Enhanced cell-based assays