FLUOstar Omega

Filter-based multi-mode microplate reader

SelectScience Best Drug Discovery Product 2010, the FLUOstar® Omega is a multimode microplate reader with five detection modes. It utilizes an ultra-fast UV/Vis spectrometer or filters for absorbance as well as highly sensitive filters for all other detection modes. The FLUOstar Omega is the ideal plate reader for life science applications.

The upgradeable high performance life science microplate reader.

Filter abs
Absorbance Microplate ReadersAlpha TechnologyFluorescence Intensity (including FRET)Luminescence Microplate Readers (including BRET)Time-Resolved FluorescenceTR-FRET
  • DLR™ Assay System
  • HTRF® - Homogenous Time-Resolved Fluorescence
  • LanthaScreen® Technology
  • Transcreener® ADP2 FI Assay
  • Transcreener® ADP2 TR-FRET Assay
  • MycoAlert


The FLUOstar Omega is a versatile multi-mode microplate reader for five detection modes: fluorescence intensity (including FRET), luminescence (including BRET), UV/Vis absorbance, time-resolved fluorescence (including TR-FRET), and AlphaScreen®/AlphaLISA®


  • Full-spectrum UV/Vis absorbance measurements in less than 1 second or filter-based absorbance
  • Measurement in up to 384-well microplate format
  • Top and bottom reading
  • Plate shaking and uniform incubation up to 45 °C or 65 °C
  • Well-scanning and real-time kinetic monitoring
  • High-precision injectors with simultaneous reagent injection and detection
  • Includes multi-user control and MARS data analysis software
  • Compatible with LVis Plate and Stacker

232, Real-Time Quaking Induced Conversion Assay for Prion Seeding -

231, Label-Free Kinetic Measurements Using SoPRano™ Gold Nano Rods (GNRs) and a Spectrometer Microplate Reader -

230, Label-Free SoPRano™ Gold Nano-Rod (GNR) Assays on a Spectrometer-Based Microplate Reader -

228, Biocolor's APOPercentage Apoptosis Assay™ on BMG LABTECH's SPECTROstar Nano plate reader -

224, Assessing pancreatic trypsin activity using the POLARstar Omega Microplate Reader -

223, Real-time Monitoring of Intracellular Oxygen Using MitoXpress®-Intra -

221, Monitoring Intracellular cAMP with hMSC cAMP biosensor using the LUMIstar Omega -

220, A Fluorescence Based Assay of the Epigenetic Enzyme Histonedeacetylase 1 (HDAC1) -

219, Assessment of Extracellular Acidification using a Fluorescence-Based Assay -

218, BlueScreen HC - A Luminescence Based, High-Throughput, In Vitro Genotoxicity Assay -

217, Growth of Campylobacter using BMG LABTECH s FLUOstar Omega equipped with ACU -

216, Monitoring Intracellular Ca2+ Fluxes with Clonetics™ primary sensors using the LUMIstar Omega -

214, Quantitative, High- Throughput, Fluorescent-Based Bioassay to Detect Schistosoma Viability -

213, The ELISAONE™ assay performed on the POLARstar Omega from BMG LABTECH -

212, Promotion of Aggregation as a Means of Assessing the Stability of Antibody Molecules -

211, Measure femtogram quantities of dsDNA on the BMG LABTECH PHERAstar Plus -

210, An AlphaScreen® SureFire® Phospho-ERK1/2 assay performed on BMG LABTECH's POLARstar Omega -

209, GPCR Activation is Measured with Cisbio’s cAMP and IP-One HTRF® HTplex™ Cell-based Assay -

208, A Fast and Simple Method For Measuring P-Glycoprotein (Pgp) Inhibition -

207, Assessing Microbial Metabolism Using A Simple ‘Mix & Measure’ Oxygen Consumption Assay -

204, Analysis of migration using the Oris(TM) Cell Migration Assay-TriCoated kit on the POLARstar Omega -

203, Enzyme Kinetic Measurements for a Combinatorial Library of Inhibitors of Pseudomonas Elastase -

202, Promega's ADP-Glo™ Kinase Assay performed on BMG LABTECH's PHERAstar FS and POLARstar Omega -

201, The Transcreener® ADP2 FI Assay performed on PHERAstar and Omega microplate readers -

199, Monitoring Bacterial Cell-to-Cell Communication “Quorum sensing” Using FLUOstar OPTIMA -

197, ORAC Assay Performed on the POLARstar Omega and PHERAstar FS microplate reader -

196, Using Intrinsic Tryptophan Fluorescence to Measure Heterotrimeric G-Protein Activation -

195, alamarBlue® Assay for Assessment of Cell Proliferation using the FLUOstar OPTIMA -

193, Functional Screening of recombinant CHO-M1 cells using the PHERAstar from BMG LABTECH -

192, A DELFIA® time-resolved fluorescence cell-mediated cytotoxicity assay performed on the PHERAstar -

191, High-Throughput Monitoring of Bacterial Growth at Elevated Hydrostatic Pressure on the FLUOstar OPTIMA -

190, Homogeneous IgG AlphaLISA® assay performed on BMG LABTECH’s PHERAstar Plus -

189, High-Throughput Method for Dual Assessment of Antifungal Activity and Growth Kinetics Using a FLUOstar Omega -

188, A Leukocyte Adhesion Assay Performed on BMG LABTECH’s FLUOstar OPTIMA -

187, Application of the Amplifluor® SNPs Genotyping System using a FLUOstar OPTIMA microplate reader -

186, AlphaScreen® Phosphotyrosine Assay Performed on a FLUOstar Omega and a POLARstar Omega -

184, Multiplex Analysis of Inflammatory Cytokines from Primary Human Macrophages using a FLUOstar Omega -

179, Fluorometric determination of extracellular enzyme activities in peat using the FLUOstar OPTIMA -

178, Reaction optimization by parallel kinetic studies with the FLUOstar Omega -

177, HTRF® IP-One Terbium-based assay performed on BMG LABTECH´s PHERAstar Plus -

176, Rapid ultra-sensitive isothermal DNA detection using RPA technology and the POLARstar OPTIMA -

175, Measuring Mitochondrial Membrane Potential using the FLUOstar Omega Microplate Reader -

172, Dual luciferase assay to assess the replication of the hepatitis C virus subgenomic replicon -

171, Evaluation of PARP Inhibitors: Performed on BMG LABTECH’s FLUOstar Omega -

169, Old Assays, New Instrument: ELISA; NADH and NADPH Conversion; DNA and Protein Quantitation -

168, DNA Absorbance Quantitation Using BMG LABTECH´s POLARstar Omega Microplate Reader -

167, Comparison of Thioredoxin Activity in Cortical Neurons and Glial Cells using the FLUOstar OPTIMA -

166, Molecular Probes® NanoOrange® Assay Performed on BMG LABTECH FLUOstar OPTIMA Microplate Reader -

165, Dual Luciferase Reporter (DLR) Assay Certification on the Omega Series of Readers -

164, Use of the FLUOstar OPTIMA Microplate Reader to Monitor Amyloid Formation -

163, SUMO FRET-based Assays Performed on BMG LABTECH’s NOVOstar -

159, High-Throughput Method for Dynamic Measurements of Cellular Viability Using a FLUOstar OPTIMA -

157, Calcium Detection Upon GPCR Stimulation Using the NOVOstar Microplate Reader and Screen Quest™ Fluo-8 NW Calcium Kit -

156, Internalisation of Fluorescently Labelled Phagocytosis Targets Quantified by the FLUOstar OPTIMA -

151, Promega’s P450-Glo™ Luminescent Cytochrome P450 Assay Using the FLUOstar OPTIMA -

148, ORAC Assay on the FLUOstar OPTIMA to Determine Antioxidant Capacity -

146, Size-selective assessment of tight junction paracellular permeability using fluorescently labelled dextrans -

145, Plasso EpranEx™ ready to use heparin-binding plate with the FLUOstar OPTIMA plate reader -

144, Analysis of Prostate Tumour Cell Invasion Using BD FluoroBlok™and FLUOstar OPTIMA -

143, Study of GPCR pharmacology using the DiscoveRx HitHunter™ cAMP HS assay on the FLUOstar OPTIMA -

141, ADP Hunter™ Assay for HTS of Kinase Inhibitors using the PHERAstar -

140, High-Throughput Determination of Bacterial Growth Kinetics using a FLUOstar OPTIMA -

137, HTRF® IP-One Assay Performed on the PHERAstar and RUBYstar* Plate Readers -

136, LUX Biotechnology GLOWELL™ luminometry standards on the POLARstar OPTIMA -

135, Protein Aggregation Monitoring on the FLUOstar OPTIMA Microplate Reader -

132, Development of a post-reaction derivatisation and fluorescence assay for 2-OG dependent dioxygenases -

131, Cambrex PKLight™ Protein Kinase Assay on the PHERAstar Plate Reader -

130, Cambrex Bio Science MycoAlert™ Assay on the LUMIstar plate reader -

128, High-Throughput Measurement of Protein Stability using a FLUOstar OPTIMA Microplate Reader -

118, BRET assays performed on the FLUOstar OPTIMA -

116, DNA Quantification (Absorbance Mode) -

113, The use of an ATP bioluminescence assay to quantify cell cytotoxicity -

112, Use of the LUMIstar to Monitor the Production of Free Radicals by Leukocytes in Diluted Blood -

111, Chemiluminescence Measurement of the Generation of Reactive Oxygen Species -

110, Promega ENLITEN Kit on the FLUOstar Galaxy and LUMIstar Galaxy -

108, Luminescent ABEL® Antioxidant Test Kit with PHOLASIN® for Vitamin C type antioxidants -

106, Inhibitory Effect of Terbinafine on Reactive Oxygen Species (ROS) Generation by Candida albicans -

105, Fluorescence analysis of reactive oxygen species by six isolates of Aspergillus Fumigatus -

104, Intracellular Calcium Assay performed on NOVOstar Microplate Reader -

103, Molecular Probes PicoGreen assay performed on BMG LABTECH POLARstar OPTIMA plate reader -

Detection Modes

Fluorescence Intensity
Luminescence (flash and glow)
Time-Resolved Fluorescence - including TR-FRET
UV/Vis Absorbance Spectra

Measurement Modes

Top and bottom reading
Endpoint and kinetic measurements
Sequential Multi Excitation measurements
Sequential Multi Emission measurements
Spectral scanning (absorbance)
Ratiometric measurements
Well scanning

Microplate Formats

6 to 384-well plates, user-definable
LVis Plate with 16 low volume microspots (2 μL)

Microplate Carrier

Robot compatible

Light Sources

High energy xenon flash lamp


Low noise photomultiplier tube
CCD spectrometer

Wavelength Selection

Optical Filters: Ex and Em wheels for 8 filters each
UV/Vis Absorbance Spectrometer: Full spectra or 8 distinct wavelengths in < 1 sec / well

Optical Filters

Excitation and emission filter wheels for 8 filters each

Optical Path Guides

Top: Liquid filled light guides
Bottom: Fiber optics

Spectral Range

Filters 240 - 750 nm or 240 - 900 nm for FI, FP, TRF
240 - 750 nm for LUM
Spectrometer 220 - 1000 nm for ABS


< 0.2 fmol/well Sodium Fluorescein 
< 30 amol/well Europium

High-End TRF for Omega

< 3 amol/well Europium
< 20 amol/well ATP

with flash lamp

< 5 pM (< 100 amol/well P-Tyr-100, 384sv, 20 μL)
with Spectrometer
Full spectrum captured in < 1 s/well
Selectable spectral resolution: 1, 2, 5, and 10 nm
OD range: 0 to 4 OD
Accuracy: < 1% at 2 OD
Precision: < 0.5% at 1 OD and < 0.8% at 2 OD

Read Times

Flying mode (1 flash)
9 s (96), 16 s (384)

Reagent Injection

Up to 2 built-in reagent injectors 
Injection at measurement position (6 to 384-well) 
Individual injection volumes for each well (3 to 500 µL) 
Variable injection speed up to 420 µL / s 
Up to four injection events per well 
Reagent back flushing


Linear, orbital, and double-orbital with user-definable time and speed

Purge Gas Vent

System to inject an atmosphere or to pull a vacuum into the reader


+4 °C above ambient up to 45 °C or 65 °C

The upper heating plate of the incubation chamber operates at 0.5 °C more than the lower plate. This prevents condensation build-up on the lid or sealer. 


Multi-user Reader Control and MARS Data Analysis Software included
FDA 21 CFR Part 11 compliant


Width: 44 cm, depth: 48 cm, height: 30 cm; weight: 28 kg

Optional Accessories

LVis Plate

Sample Capacity: Sixteen separate microdrop wells for 2 µL samples; One standard cuvette position for up to 1 mL samples.

Quality Control Internal Standards (optional): Four NIST traceable optical density filters (approximate values of 0.1, 0.3, 0.6 and 1.0 OD); One holmium oxide filter for wavelength accuracy

Dimensions: Conforms to SBS standards for microplates.


Magazines for up to 50 plates - continuous loading feature


Microplate Incubator and Shaker

Optical Filters

Excitation and emission filter wheels for 8 filters each


Please contact your local representative for upgrades including options such as detection modes, reagent injectors, etc.


LOD = 3 x SD (20 blanks) / slope (6 pt std curve)
AlphaScreen® P-Tyr-100 assay kit, PerkinElmer, #6760620C
Microplates: white for LUM, AlphaScreen®, TRF; black for FI, FP; clear for ABS
96 = 96-well microplates
384sv = 384-well small volume microplates
384g = 384-well glass bottom microplates
  • DLR™ Assay System
  • HTRF® - Homogenous Time-Resolved Fluorescence
  • LanthaScreen® Technology
  • Transcreener® ADP2 FI Assay
  • Transcreener® ADP2 TR-FRET Assay
  • MycoAlert