Colorimetric and turbidimetric analysis of endotoxins using the absorbance detection mode
Torn between a colorimetric or turbidimetric LAL assay? Whichever path you take, BMG LABTECH’s absorbance readers deliver dependable results every time.
El PHERAstar FSX es el lector multimodo más novedoso y rápido para cribado de alto rendimiento, combina su altísima sensibilidad con rapidísimos tiempos de lectura. Gracias al sistema único de módulo óptico, Emisión dual simultánea, tecnología de rango dinámico ampliado, espectrómetro UV/vis, sistema AAS y los láseres específicos AlphaScreen® y TRF, el PHERAstar FSX realizará su ensayo con rapidez y facilidad, hasta en microplacas de 3456 pocillos.


Ya no tendrá que preocuparse por instalar filtros y dicroicos: el PHERAstar FSX incluye módulos ópticos fáciles de manejar y optimizados para ensayos que contienen todos los filtros, espejos, dicroicos y/o polarizadores específicos para cada aplicación, y que el lector de placas reconoce automáticamente. Además, el PHERAstar FSX está equipado con cuatro tubos fotomultiplicadores (PMTs) adaptados y optimizados para ensayos, que se seleccionan automáticamente para el modo de detección correspondiente. También puede medir ensayos con dos longitudes de onda de emisión al mismo tiempo gracias a la emisión dual simultánea.
Las tecnologías de rango dinámico ampliado y enfoque automático hacen que los ajustes manuales sean superfluos y la optimización de la detección más fácil que nunca.
Para fines de automatización de HTS, el PHERAstar FSX se puede integrar con facilidad en sistemas automatizados, y ofrece capacidades mejoradas de integración robótica y control multiusuario.
El PHERAstar FSX es solo para uso en investigación.

Torn between a colorimetric or turbidimetric LAL assay? Whichever path you take, BMG LABTECH’s absorbance readers deliver dependable results every time.
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Fluorescence intensity - incl. FRET
Fluorescence polarization
AlphaScreen®/AlphaLISA®/AlphaPlex™
Luminescence (flash and glow) - incl. BRET
Time-resolved fluorescence
TR-FRET
UV/Vis absorbance spectra
Top and bottom reading
Endpoint and kinetic
Sequential multi-excitation
Sequential multi-emission
Simultaneous Dual Emission
Spectral scanning (absorbance)
Real-time ratiometric measurements
Well scanning
Up to 3456-well plates, user-definable
LVis Plate with 16 low volume microspots (2 μL)
Robot compatible
High energy xenon flash lamp
Dedicated laser for AlphaScreen®/AlphaLISA®/AlphaPlex™
Dedicated laser for TRF and TR-FRET
Two matched pairs of photomultiplier tubes (PMTs), optimized for different detection modes
CCD spectrometer
Optic Modules: Up to six application-specific modules (includes all filters, dichroics, etc. for an application)
For top and bottom reading
UV/vis absorbance spectrometer: Full spectra or 8 distinct wavelengths in < 1 sec/well
Filters
230 - 750 nm or 230 - 900 nm for FI, FP
230 - 750 nm for LUM
230 - 900 nm for TRF
Spectrometer
220 - 1000 nm for ABS; wavelength precision: ≤ 0.5 nm
FI
(top)
< 0.15 pM FITC (384sv black, 20 μL)
< 0.5 pM FITC (1536 black, 8 μL)
Dynamic Range: 8 decades
FI
(bottom)
<1.0 pM FITC (384g black, 50 μL)
FP
< 0.5 mP SD at 1 nM FITC (384sv black, 20 μL)
< 1.5 mP SD at 1 nM FITC (1536 black, 8 μL)
TRF with laser.
< 5 fM europium (384, 80 μL)
< 15 fM europium (1536, 8 μL)
HTRF®
(black and white microplates)
Reader Control Kit (Eu) after 18h (384sv, 20 μL)
Delta F > 1100% (High Calibrator)
Delta F > 25% (Low Calibrator)
LUM
< 0.4 pM (< 8 amol/well ATP, 384sv white, 20 μL)
Dynamic Range: 8 decades
AlphaScreen® with laser
< 5 pM (< 100 amol/well P-Tyr-100, 384sv white, 20 μL)
ABS with spectrometer
Selectable spectral resolution: 1, 2, 5, and 10 nm
OD range: 0 to 4 OD; photometric resolution: 0.001 OD
Accuracy: < 1% at 2 OD
Precision: < 0.5% at 1 OD and < 0.8% at 2 OD
Linearity: ≤ 0.8% at 2.0 OD
Flying mode (1 flash)
14 s (384)
27 s (1536)
10 flashes
38 s (384)
1 min 52 s (1536)
50 flashes
1 min 29 s (384)
5 min 18 s (1536)
Linear, orbital, and double-orbital with user-definable time and speed
+5 °C above ambient up to 45 °C
The upper heating plate of the incubation chamber operates at +0.5 °C more than the lower plate. This prevents condensation build-up on the lid or sealer
With Advanced Assay Stability (AAS) system: from 18 - 45°C
Multi-user Reader Control and MARS Data Analysis Software included
FDA 21 CFR Part 11 compliant
Width: 45 cm, depth: 51 cm, height: 47 cm; weight: 49 kg
With AAS system: width: 48 cm, depth: 68 cm, height: 47 cm; weight: 62 kg
Top and bottom: free-air optical light path guided by motor-driven mirrors and dichroics
Automatic focal height adjustment (0.1 mm resolution)
Up to 2 built-in reagent injectors
Injection at measurement position (6 to 384-well)
Individual injection volumes for each well (3 to 500 µL, optionally up to 2 mL)
Variable injection speed up to 420 µL/s
Up to four injection events per well
Reagent back flushing
Up to three integrated barcode readers
Sample Capacity: sixteen separate microdrop wells for 2 µL samples; one standard cuvette position for up to 1 mL samples
Quality Control Internal Standards (optional): four NIST traceable optical density filters (approximate values of 0.1, 0.3, 0.6 and 1.0 OD); one holmium oxide filter for wavelength accuracy
Dimensions: conforms to SBS standards for microplates
Microplate incubator and shaker
Magazines for up to 50 plates - continuous loading feature
Please contact your local representative for upgrades including options such as detection modes, reagent injectors, etc
LOD = 3 x SD (20 blanks) / slope (6 pt std curve)
AlphaScreen® P-Tyr-100 assay kit, PerkinElmer, #6760620C
384sv = 384-well small volume microplates
384g = 384-well glass bottom microplates
1536 = 1536-well microplates
The PHERAstar FSX is for research use only.
Intelligent microplate incubation
Microplate handling system
Low-volume measurements
Filters for a wide range of applications
Functional Microplate Reader Validation