
Determination of Minimum Inhibitory Concentration (MIC) of antibiotics using OD600
Do you want to make your MIC measurements more efficient and automated? Find out here how BMG LABTECH microplate readers can support you in this task.
El PHERAstar FSX es el lector más novedoso y rápido para High-Throughput Screening, combina su altísima sensibilidad con rapidísimos tiempos de lectura. Gracias al sistema único de módulo óptico, Emisión dual simultanea, Tecnología de Rango Dinámico Incrementado, espectrometro UV/vis, sistema AAS y AlphaScreen® y TRF lásers dedicados, el PHERAstar FSX realizará su ensayo con rapidez y facilidad, hasta en microplacas de 3456 pocillos.
No se preocupe por que filtro o dicróico esté instalado en su PHERAstar FSX. Los Módulos Ópticos son muy fáciles de manejar y contienen los filtros, espejos, dicróicos y/o polarizadores para cada aplicación y son reconocidos automáticamente por el lector. Además el PHERAstar FSX está equipado con 4 tubos fotomultiplicadores (PMTs) que son seleccionados automáticamente para cada modo de detección, así como para Emisión Simultanea Dual.
El Rango Dinámico Incrementado y la tecnología de enfoque automático hacen la configuración manual superflua y la optimización de la detección más fácil que nunca.
Para fines de automatización de HTS, el PHERAstar FSX pede ser integrado con facilidad en sistemas automatizados, y ofrece capacidades mejoradas de integracion robótica y control multiusuario.
El PHERAstar FSX es solo para uso en investigación.
Do you want to make your MIC measurements more efficient and automated? Find out here how BMG LABTECH microplate readers can support you in this task.
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Intramolecular bivalent glues can be used for the targeted degradation of proteins. Find out how their binding behaviour can be investigated using microplate readers.
Antibiotic resistance continues to increase. Find out here how BOCILLIN can be used to study resistant penicillin binding protein variants using fluorescence polarization.
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Castro, A;Ruiz-Rivera, A;Moorman, C;Wolf-Saxon, E;Mims, H;Vasquez-Meza, V;Rangel, M;Loera, M;Bond, I;Buchanan, S;Villarreal, E;Tripathi, S;Rubin, S;Burke, J; 2024
J Biol Chem
Xie, S;Tai, C;Morton, C;Ma, L;Huang, S;Wittlin, S;Du, Y;Hu, Y;Dogovski, C;Salimimarand, M;Griffin, R;England, D;de la Cruz, E;Deni, I;Yeo, T;Burkhard, A;Striepen, J;Schindler, K;Crespo, B;Gamo, F;Khandokar, Y;Hutton, C;Rabie, T;Birkholtz, L;Famodimu, M;Delves, M;Bolsher, J;Koolen, K;van der Laak, R;Aguiar, A;Pereira, D;Guido, R;Creek, D;Fidock, D;Dick, L;Brand, S;Gould, A;Langston, S;Griffin, M;Tilley, L; 2024
PLoS Pathog
Tang, B;Lam, B;Holley, S;Torres, M;Kuntzweiler, T;Gladysheva, T;Lang, P; 2024
SLAS technology 100233
Qi, F;Zhang, M;Yang, G;Wang, W;Hu, Y;Shen, Y;Wan, J;Li, J;Liu, G;Deng, Y; 2024
Fitoterapia 106332
Piki, E;Dini, A;Rantanen, F;Bentz, F;Paavolainen, L;Barker, H;Raivola, J;Hirasawa, A;Kallioniemi, O;Murumägi, A;Ungureanu, D; 2024
Molecular therapy. Oncology 32(4) 200903
Nair, JR;Huang, TT;Sunkara, A;Pruitt, MR;Ibanez, KR;Chiang, CY;Cheng, KC;Wilson, K;Cardillo, TM;Hofsess, S;Lee, JM; 2024
iScience 27(12) 111283
Fluorescence intensity - incl. FRET
Fluorescence polarization
AlphaScreen®/AlphaLISA®/AlphaPlex™
Luminescence (flash and glow) - incl. BRET
Time-resolved fluorescence
TR-FRET
UV/Vis absorbance spectra
Top and bottom reading
Endpoint and kinetic
Sequential multi-excitation
Sequential multi-emission
Simultaneous Dual Emission
Spectral scanning (absorbance)
Real-time ratiometric measurements
Well scanning
Up to 3456-well plates, user-definable
LVis Plate with 16 low volume microspots (2 μL)
Robot compatible
High energy xenon flash lamp
Dedicated laser for AlphaScreen®/AlphaLISA®/AlphaPlex™
Dedicated laser for TRF and TR-FRET
Two matched pairs of photomultiplier tubes (PMTs), optimized for different detection modes
CCD spectrometer
Optic Modules: Up to six application-specific modules (includes all filters, dichroics, etc. for an application)
For top and bottom reading
UV/vis absorbance spectrometer: Full spectra or 8 distinct wavelengths in < 1 sec/well
Filters
230 - 750 nm or 230 - 900 nm for FI, FP
230 - 750 nm for LUM
230 - 900 nm for TRF
Spectrometer
220 - 1000 nm for ABS; wavelength precision: ≤ 0.5 nm
FI
(top)
< 0.15 pM FITC (384sv black, 20 μL)
< 0.5 pM FITC (1536 black, 8 μL)
Dynamic Range: 8 decades
FI
(bottom)
<1.0 pM FITC (384g black, 50 μL)
FP
< 0.5 mP SD at 1 nM FITC (384sv black, 20 μL)
< 1.5 mP SD at 1 nM FITC (1536 black, 8 μL)
TRF with laser.
< 5 fM europium (384, 80 μL)
< 15 fM europium (1536, 8 μL)
HTRF®
(black and white microplates)
Reader Control Kit (Eu) after 18h (384sv, 20 μL)
Delta F > 1100% (High Calibrator)
Delta F > 25% (Low Calibrator)
LUM
< 0.4 pM (< 8 amol/well ATP, 384sv white, 20 μL)
Dynamic Range: 8 decades
AlphaScreen® with laser
< 5 pM (< 100 amol/well P-Tyr-100, 384sv white, 20 μL)
ABS with spectrometer
Selectable spectral resolution: 1, 2, 5, and 10 nm
OD range: 0 to 4 OD; photometric resolution: 0.001 OD
Accuracy: < 1% at 2 OD
Precision: < 0.5% at 1 OD and < 0.8% at 2 OD
Linearity: ≤ 0.8% at 2.0 OD
Flying mode (1 flash)
14 s (384)
27 s (1536)
10 flashes
38 s (384)
1 min 52 s (1536)
50 flashes
1 min 29 s (384)
5 min 18 s (1536)
Linear, orbital, and double-orbital with user-definable time and speed
+5 °C above ambient up to 45 °C
The upper heating plate of the incubation chamber operates at +0.5 °C more than the lower plate. This prevents condensation build-up on the lid or sealer
With Advanced Assay Stability (AAS) system: from 18 - 45°C
Multi-user Reader Control and MARS Data Analysis Software included
FDA 21 CFR Part 11 compliant
Width: 45 cm, depth: 51 cm, height: 47 cm; weight: 49 kg
With AAS system: width: 48 cm, depth: 68 cm, height: 47 cm; weight: 62 kg
Top and bottom: free-air optical light path guided by motor-driven mirrors and dichroics
Automatic focal height adjustment (0.1 mm resolution)
Up to 2 built-in reagent injectors
Injection at measurement position (6 to 384-well)
Individual injection volumes for each well (3 to 500 µL, optionally up to 2 mL)
Variable injection speed up to 420 µL/s
Up to four injection events per well
Reagent back flushing
Up to three integrated barcode readers
Sample Capacity: sixteen separate microdrop wells for 2 µL samples; one standard cuvette position for up to 1 mL samples
Quality Control Internal Standards (optional): four NIST traceable optical density filters (approximate values of 0.1, 0.3, 0.6 and 1.0 OD); one holmium oxide filter for wavelength accuracy
Dimensions: conforms to SBS standards for microplates
Microplate incubator and shaker
Magazines for up to 50 plates - continuous loading feature
Please contact your local representative for upgrades including options such as detection modes, reagent injectors, etc
LOD = 3 x SD (20 blanks) / slope (6 pt std curve)
AlphaScreen® P-Tyr-100 assay kit, PerkinElmer, #6760620C
384sv = 384-well small volume microplates
384g = 384-well glass bottom microplates
1536 = 1536-well microplates
The PHERAstar FSX is for research use only.
Intelligent microplate incubation
Microplate handling system
Low-volume measurements
Filters for a wide range of applications
Functional Microplate Reader Validation
Fluorescence intensity - incl. FRET
Fluorescence polarization
AlphaScreen®/AlphaLISA®/AlphaPlex™
Luminescence (flash and glow) - incl. BRET
Time-resolved fluorescence
TR-FRET
UV/Vis absorbance spectra
Top and bottom reading
Endpoint and kinetic
Sequential multi-excitation
Sequential multi-emission
Simultaneous Dual Emission
Spectral scanning (absorbance)
Real-time ratiometric measurements
Well scanning
Up to 3456-well plates, user-definable
LVis Plate with 16 low volume microspots (2 μL)
Robot compatible
High energy xenon flash lamp
Dedicated laser for AlphaScreen®/AlphaLISA®/AlphaPlex™
Dedicated laser for TRF and TR-FRET
Two matched pairs of photomultiplier tubes (PMTs), optimized for different detection modes
CCD spectrometer
Optic Modules: Up to six application-specific modules (includes all filters, dichroics, etc. for an application)
For top and bottom reading
UV/vis absorbance spectrometer: Full spectra or 8 distinct wavelengths in < 1 sec/well
Filters
230 - 750 nm or 230 - 900 nm for FI, FP
230 - 750 nm for LUM
230 - 900 nm for TRF
Spectrometer
220 - 1000 nm for ABS; wavelength precision: ≤ 0.5 nm
FI
(top)
< 0.15 pM FITC (384sv black, 20 μL)
< 0.5 pM FITC (1536 black, 8 μL)
Dynamic Range: 8 decades
FI
(bottom)
<1.0 pM FITC (384g black, 50 μL)
FP
< 0.5 mP SD at 1 nM FITC (384sv black, 20 μL)
< 1.5 mP SD at 1 nM FITC (1536 black, 8 μL)
TRF with laser.
< 5 fM europium (384, 80 μL)
< 15 fM europium (1536, 8 μL)
HTRF®
(black and white microplates)
Reader Control Kit (Eu) after 18h (384sv, 20 μL)
Delta F > 1100% (High Calibrator)
Delta F > 25% (Low Calibrator)
LUM
< 0.4 pM (< 8 amol/well ATP, 384sv white, 20 μL)
Dynamic Range: 8 decades
AlphaScreen® with laser
< 5 pM (< 100 amol/well P-Tyr-100, 384sv white, 20 μL)
ABS with spectrometer
Selectable spectral resolution: 1, 2, 5, and 10 nm
OD range: 0 to 4 OD; photometric resolution: 0.001 OD
Accuracy: < 1% at 2 OD
Precision: < 0.5% at 1 OD and < 0.8% at 2 OD
Linearity: ≤ 0.8% at 2.0 OD
Flying mode (1 flash)
14 s (384)
27 s (1536)
10 flashes
38 s (384)
1 min 52 s (1536)
50 flashes
1 min 29 s (384)
5 min 18 s (1536)
Linear, orbital, and double-orbital with user-definable time and speed
+5 °C above ambient up to 45 °C
The upper heating plate of the incubation chamber operates at +0.5 °C more than the lower plate. This prevents condensation build-up on the lid or sealer
With Advanced Assay Stability (AAS) system: from 18 - 45°C
Multi-user Reader Control and MARS Data Analysis Software included
FDA 21 CFR Part 11 compliant
Width: 45 cm, depth: 51 cm, height: 47 cm; weight: 49 kg
With AAS system: width: 48 cm, depth: 68 cm, height: 47 cm; weight: 62 kg
Top and bottom: free-air optical light path guided by motor-driven mirrors and dichroics
Automatic focal height adjustment (0.1 mm resolution)
Up to 2 built-in reagent injectors
Injection at measurement position (6 to 384-well)
Individual injection volumes for each well (3 to 500 µL, optionally up to 2 mL)
Variable injection speed up to 420 µL/s
Up to four injection events per well
Reagent back flushing
Up to three integrated barcode readers
Sample Capacity: sixteen separate microdrop wells for 2 µL samples; one standard cuvette position for up to 1 mL samples
Quality Control Internal Standards (optional): four NIST traceable optical density filters (approximate values of 0.1, 0.3, 0.6 and 1.0 OD); one holmium oxide filter for wavelength accuracy
Dimensions: conforms to SBS standards for microplates
Microplate incubator and shaker
Magazines for up to 50 plates - continuous loading feature
Please contact your local representative for upgrades including options such as detection modes, reagent injectors, etc
LOD = 3 x SD (20 blanks) / slope (6 pt std curve)
AlphaScreen® P-Tyr-100 assay kit, PerkinElmer, #6760620C
384sv = 384-well small volume microplates
384g = 384-well glass bottom microplates
1536 = 1536-well microplates
The PHERAstar FSX is for research use only.