Most flexible plate reader with LVF monochromators
The CLARIOstar® is BMG LABTECH’s most flexible microplate reader, equipped with our revolutionary LVF monochromator™ technology. A combination of monochromators, filters, and spectrometer means that it does not compromise on sensitivity or flexibility. As well as its high performance in all detection modes, the CLARIOstar’s versatility also makes it ideal for assay development.
The CLARIOstar is a multi-mode microplate reader with advanced LVF Monochromators™, highly sensitive filters, and an ultra-fast UV/vis spectrometer. It is a modular microplate reader with up to seven different detection modes: fluorescence intensity (incl. FRET), fluorescence polarization, luminescence (including BRET), UV/vis absorbance, time-resolved fluorescence, TR-FRET, and AlphaScreen®/AlphaLISA®/AlphaPlex™.
- Innovative monochromators for increased sensitivity
- Fully-flexible wavelengths and bandwidths
- Spectral scanning in AS, FI and LUM
- Higher light transmission
- High-speed absorbance measurements
- Dedicated laser for Alpha Technology
- Live cell-based assays
- Low volume measurements
Measuring changes in cellular metabolism by monitoring extracellular acidification and oxygen consumption in real-timeDavid Hoffman (1) and Carl Peters (2), 1) Cayman Chemical , Ann Arbor , MI 2) BMG LABTECH Inc. , NC , USA, 05/2017
Dysregulation of cellular energy metabolism is a common factor in a variety of disorders including diabetes and obesity as well as cancer. The current technologies used to assess mitochondrial function via extracellular acidification (ECA) and oxygen consumption rate (OCR) are often designed in such a way that they ignore the impact of O2 concentration on cellular bioenergetics.
Here we describe the implementation of the CLARIOstar® with ACU in conjunction with advanced dyes from Luxcel Biosciences to measure O2 consumption and glycolytic flux in an open-flow respirometry system. This system enables measurement of respiration rate at steady state, where O2 supplied equals O2 consumed.
The Mitochondrial Stress Test (ACU format) from Cayman Chemical combined with the CLARIOstar and ACU provides a "push button" multiplexed system with the potential for monitoring additional cell health outcomes. Experiments can be performed under user defined [O2], using either adherent cells or cell suspensions.
Detection of plant-synthesized nanoparticles and their antibacterial capacitySalem W. and Schild S., University of Graz , Institute of Molecular Biosciences , BioTechMed-Graz , Austria, 03/2017
Metallic nanoparticles became subject of intensive research because of their potential antibiotic properties. Nanoparticles such as silver, gold or zinc oxide particles are easily and cost-effectively synthesized by blending metal salts with plant extracts that reduce the metal. Different extracts, varying in the plant or the part of the plant used for the extract, are currently investigated in regard to their capacity to form nanoparticles and their antimicrobial efficacy. The formation of nanoparticles can be verified by UV-Vis spectroscopy due to surface plasmon resonance of the particles that lead to a characteristic spectrum defined by the underlying metal and particle size. Subsequent analysis of nanoparticles on microbial growth is typically tested by methods based on absorbance changes.
Here, we present how the spectrometer-based BMG LABTECH instruments are used to quickly confirm Ag and ZnO nanoparticle formation and their inhibitory effect on the diarrhea-causing bacteria Vibrio cholerae and enterotoxic Escherichia coli (ETEC).
The fluorescent probe NR12S detects changes in plasma membrane cholesterol levelsWendy S. Smith , Sopsamorn U. Flavell and David J. Flavell, The Simon Flavell Leukaemia Research Laboratory , Southampton General Hospital , Southampton , Hampshire , SO16 6YD, 02/2017
The cell membrane is a bilayer of phospholipids with embedded proteins. It contains cholesterol that determines the membrane's fluidity, permeability and activity of membrane proteins. Changes in membrane cholesterol are implicated in diseases such as Alzheimer's and cancer, demanding its investigation.
NR12S is a fluorophore that exhibits emission maxima at 560 and 630 nm dependent on the liquid order of the membrane. Incorporation of the dye in a liquid ordered phase (increased cholesterol) shifts the emission to 560 nm, whereas in a liquid disordered phase (lower cholesterol) it emits at 630 nm. Hence, the 560/630 ratio correlates with the membrane cholesterol content.
Inhibition of cholesterol synthesis by lovastatin or incubation with methyl-§-cyclodextrin decreased membrane cholesterol in hematological cells and was reported by decreases in the 560/630 ratio. Both emissions were conveniently measured on the filter-based FLUOstar® Omega microplate reader and could be analyzed with one-click using the MARS analysis software.
Simultaneous detection of GPCR second messengers in living cellsP. Tewson (1) , S. Martinka (1) , S. Tillo (1) , T. Hughes (1) , A.M. Quinn (1) , C. Peters (2), 1) Montana Molecular , Bozeman , MT 2) BMG LABTECH , Cary , NC, 12/2016
Due to their role in signal transmission from the outside to the inside of a cell, G-protein coupled receptors are prominent drug targets. Signaling from a stimulated Gq-coupled receptor activates Phospholipase C (PLC) and results in production of the second messengers PIP2, DAG and finally in the release of Ca2+.
Montana Molecular developed genetically encoded biosensors that decrease (downward) or increase (upward) fluorescence in presence of the specific Gq associated second messengers. They are available in red and green to enable multiplexing.
The sensors were expressed in HEK293 cells and the acetylcholine receptor was activated by Carbachol. Generation of DAG, PIP2 and Ca2+ was reliably reported by the biosensors and the CLARIOstar® proved a suitable detector: filter-based and monochromator-based measurements were sensitive and fast enough to detect changes of up to two second messengers upon carbachol injection in one well, necessary for unambiguous identification of Gq signaling.
Verifying SPARCL performance on the CLARIOstar equipped for reading at time of injectionMark Cameron (1) , Carl Peters (2), 1) Lumigen , 2) BMG LABTECH, 11/2016
SPARCL refers to an immunoassay that does not require washing steps. An analyte in a solution is detected by binding two antibodies: one is coupled to horse radish peroxidase (HRP), the other is coupled to the HRP substrate Acridan. In the presence of the analyte, binding of both antibodies brings the enzyme and substrate into proximity. The enzymatic reaction is started by the addition of H2O2, which immediately produces a light proportional to the amount of analyte. Thus, SPARCL assays require to be read at the same time as the injection of the trigger solution.
The CLARIOstar® microplate reader, which is equipped with the dedicated H3 injection needle for simultaneous injection and reading, fulfils this task. The analyte IgG was detected down to a concentration of 45 ng/ml in 96 wells and down to 15.6 ng/ml in 384 well format. BMG LABTECH's MARS analysis software facilitates subsequent calculation of analyte concentration.