Second Messenger Signaling

June 10, 2019

Second messengers are intracellular molecules that transmit signals triggered by extracellular "first" messengers. We explain how fast second messenger responses are measured on a microplate reader.

Image of Dr. Andrea Krumm
Dr Andrea Krumm
PhD, Application Specialist

Easily combine different fluorophores or detection modes in one experiment

State-of-the-art assays detect biological reactions at the site of action and in real-time. This can be accomplished by genetically encoded biosensors that report biological changes directly in the cell.

Montana Molecular developed fluorescent biosensors that not only detect GPCR-dependent changes in second messengers but also enable parallel monitoring of these. The sensors detect Gs- and Gi-related cyclic adenosine monophosphate (cAMP) as well as Gq-related diacylglycerol (DAG), phosphatidylinositol-biphosphate (PIP2) and calcium ions (Ca2+). The sensors are available in red and green fluorescing versions to use them in a multiplexed assay.

The simplicity of combining different fluorophores in a kinetic measurement with BMG LABTECH instruments qualifies them to read real-time multiplex assays.


Request more information

Explore how the CLARIOstar® detects second messenger responses to unambiguously identify specific GPCR-signaling.

Find below some further materials about second messengers.