Multiplexed second messenger assays
Easily combine different fluorophores or detection modes in one experiment
State-of-the-art assays detect biological reactions at the site of action and in real-time. This can be accomplished by genetically encoded biosensors that report biological changes directly in the cell.
Montana Molecular developed fluorescent biosensors that not only detect GPCR-dependent changes in second messengers but also enable parallel monitoring of these. The sensors detect Gs- and Gi-related cyclic adenosine monophosphate (cAMP) as well as Gq-related diacylglycerol (DAG), phosphatidylinositol-biphosphate (PIP2) and calcium ions (Ca2+). The sensors are available in red and green fluorescing versions to use them in a multiplexed assay.
The simplicity of combining different fluorophores in a kinetic measurement with BMG LABTECH instruments qualifies them to read real-time multiplex assays.
Explore how the CLARIOstar® detects second messenger responses to unambiguously identify specific GPCR-signaling.
Find below some further materials about second messengers.