Fluorescence intensity - incl. FRET
Fluorescence polarization
AlphaScreen®/AlphaLISA®/AlphaPlex™
Luminescence (flash and glow) - incl. BRET
Time-resolved fluorescence
TR-FRET
UV/Vis absorbance spectra
Top and bottom reading
Endpoint and kinetic
Sequential multi-excitation
Sequential multi-emission
Simultaneous Dual Emission
Spectral scanning (absorbance)
Real-time ratiometric measurements
Well scanning
Up to 3456-well plates, user-definable
LVis Plate with 16 low volume microspots (2 μL)
Robot compatible
High energy xenon flash lamp
Dedicated laser for AlphaScreen®/AlphaLISA®/AlphaPlex™
Dedicated laser for TRF and TR-FRET
Two matched pairs of photomultiplier tubes (PMTs), optimized for different detection modes
CCD spectrometer
Optic Modules: Up to six application-specific modules (includes all filters, dichroics, etc. for an application)
For top and bottom reading
UV/vis absorbance spectrometer: Full spectra or 8 distinct wavelengths in < 1 sec/well
Filters
230 - 750 nm or 230 - 900 nm for FI, FP
230 - 750 nm for LUM
230 - 900 nm for TRF
Spectrometer
220 - 1000 nm for ABS; wavelength precision: ≤ 0.5 nm
FI
(top)
< 0.15 pM FITC (384sv black, 20 μL)
< 0.5 pM FITC (1536 black, 8 μL)
Dynamic Range: 8 decades
FI
(bottom)
<1.0 pM FITC (384g black, 50 μL)
FP
< 0.5 mP SD at 1 nM FITC (384sv black, 20 μL)
< 1.5 mP SD at 1 nM FITC (1536 black, 8 μL)
TRF with laser.
< 5 fM europium (384, 80 μL)
< 15 fM europium (1536, 8 μL)
HTRF®
(black and white microplates)
Reader Control Kit (Eu) after 18h (384sv, 20 μL)
Delta F > 1100% (High Calibrator)
Delta F > 25% (Low Calibrator)
LUM
< 0.4 pM (< 8 amol/well ATP, 384sv white, 20 μL)
Dynamic Range: 8 decades
AlphaScreen® with laser
< 5 pM (< 100 amol/well P-Tyr-100, 384sv white, 20 μL)
ABS with spectrometer
Selectable spectral resolution: 1, 2, 5, and 10 nm
OD range: 0 to 4 OD; photometric resolution: 0.001 OD
Accuracy: < 1% at 2 OD
Precision: < 0.5% at 1 OD and < 0.8% at 2 OD
Linearity: ≤ 0.8% at 2.0 OD
Flying mode (1 flash)
14 s (384)
27 s (1536)
10 flashes
38 s (384)
1 min 52 s (1536)
50 flashes
1 min 29 s (384)
5 min 18 s (1536)
Linear, orbital, and double-orbital with user-definable time and speed
+5 °C above ambient up to 45 °C
The upper heating plate of the incubation chamber operates at +0.5 °C more than the lower plate. This prevents condensation build-up on the lid or sealer
With Advanced Assay Stability (AAS) system: from 18 - 45°C
Multi-user Reader Control and MARS Data Analysis Software included
FDA 21 CFR Part 11 compliant
Width: 45 cm, depth: 51 cm, height: 47 cm; weight: 49 kg
With AAS system: width: 48 cm, depth: 68 cm, height: 47 cm; weight: 62 kg
Top and bottom: free-air optical light path guided by motor-driven mirrors and dichroics
Automatic focal height adjustment (0.1 mm resolution)
Up to 2 built-in reagent injectors
Injection at measurement position (6 to 384-well)
Individual injection volumes for each well (3 to 500 µL, optionally up to 2 mL)
Variable injection speed up to 420 µL/s
Up to four injection events per well
Reagent back flushing
Up to three integrated barcode readers
Sample Capacity: sixteen separate microdrop wells for 2 µL samples; one standard cuvette position for up to 1 mL samples
Quality Control Internal Standards (optional): four NIST traceable optical density filters (approximate values of 0.1, 0.3, 0.6 and 1.0 OD); one holmium oxide filter for wavelength accuracy
Dimensions: conforms to SBS standards for microplates
Microplate incubator and shaker
Magazines for up to 50 plates - continuous loading feature
Please contact your local representative for upgrades including options such as detection modes, reagent injectors, etc
LOD = 3 x SD (20 blanks) / slope (6 pt std curve)
AlphaScreen® P-Tyr-100 assay kit, PerkinElmer, #6760620C
384sv = 384-well small volume microplates
384g = 384-well glass bottom microplates
1536 = 1536-well microplates
The PHERAstar FSX is for research use only.
Nick Bland, Group Leader at Domainex, Cambridge, UK
