Second Messenger Signaling

Second messengers are intracellular molecules that transmit signals triggered by extracellular "first" messengers. We explain how fast second messenger responses are measured on a microplate reader.

Dr Andrea Krumm Dr Andrea Krumm (10)

 

Easily combine different fluorophores or detection modes in one experiment

State-of-the-art assays detect biological reactions at the site of action and in real-time. This can be accomplished by genetically encoded biosensors that report biological changes directly in the cell.

Montana Molecular developed fluorescent biosensors that not only detect GPCR-dependent changes in second messengers but also enable parallel monitoring of these. The sensors detect Gs- and Gi-related cyclic adenosine monophosphate (cAMP) as well as Gq-related diacylglycerol (DAG), phosphatidylinositol-biphosphate (PIP2), and calcium ions (Ca2+). The sensors are available in red and green fluorescing versions to use in a multiplexed assay.

The simplicity of combining different fluorophores in a kinetic measurement with BMG LABTECH instruments qualifies them to read real-time multiplex assays.

 
Microplate readers like the CLARIOstar®Plus and the VANTAstar™ are ideally suited to detect second messenger responses to unambiguosly identify specific GPCR-signaling.
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