Ions can travel through the membrane using ion channels. Their activity is measured with fluorescent assays and a BMG LABTECH microplate reader.
Cells not only receive extracellular cues, they also translate them into intracellular reactions. Extracellular signaling molecules comprise growth factors, hormones, ions, neurotransmitters, or cytokines.
Cellular reaction time covers from a few seconds to several hours. Accordingly, responses must either be triggered and measured instantly, or they need to be observed in living cells over a longer period of time. BMG LABTECH microplate readers provide injectors and a high sampling rate if fast reactions need to be captured. Further, the Atmospheric Control Unit regulates CO2 and O2 levels inside the reading chamber to provide a feel-good atmosphere for long-term cell experiments.
Signaling events rarely come alone, they take place simultaneously or in concert. In order to clearly characterize signaling events, BMG LABTECH devices offer the possibility of reading several assays in just one well.
Search our Resources section for information about specific applications, literature citations, videos, blog articles and many other publications. Many of the resources provided are associated with current and previous instrument models and versions.
Time Resolved Fluorescence (TRF) immunoassay in 384-well format using a matched antibody pair kit and the PHERAstar FSXJamie Campbell (1) , Oliver Carney (2) , , (1) Abcam, Cambridge, UK , (2) BMG LABTECH, Aylesbury, UK, 12/2020
Immuno-oncology biomarker measurement with high sensitivity and speed using SimpleStep ELISA kits and SPECTROstar NanoMallori Milligan (1) , Scott Detmer (1) , Carl Peters (2), (1) Abcam, Cambridge, UK , (2) BMG LABTECH, Cary, NC, 06/2020
Dual Channel Kinetic assays for detecting ligand bias at GPCRsPaul Tewson (1) , Scott Martinka (1) , Kevin M. Harlen (1) , Thom Hughes (1) , Anne Marie Quinn (1) , Sam R.J. Hoare (2) , Carl Peters (3), (1) Montana Molecular, Bozeman, MT , (2) Pharmechanics, Owego, NY , (3) BMG LABTECH Inc., NC, USA, 11/2019
Highly sensitive ELISAs in 90-minutes: SimpleStep ELISA kits and the SPECTROstar NanoMallori Milligan (1) , Scott Detmer (1) , Carl Peters (2), (1) Abcam, Cambridge, UK , (2) BMG LABTECH, Cary, NC, 10/2019
Monitoring intracellular calcium using fluorescent dyes in a mid-throughput assayThomas P. Keeley , Ron Jacob , Giovanni E. Mann , King’s BHF Centre of Research Excellence, School of Cardiovascular Medicine & Sciences, King’s College London, London SE1 9NH, UK, King’s BHF Centre of Research Excellence, School of Cardiovascular Medicine and Sciences, King’s College London, 03/2019
Second Messenger Signaling
Biosensors for Analytical Purposes
Structural basis of GABAB receptor-Gi protein couplingRead article
A STING antagonist modulating the interaction with STIM1 blocks ER-to-Golgi trafficking and inhibits lupus pathologyRead article
Targeting alveolar-specific succinate dehydrogenase A attenuates pulmonary inflammation during acute lung injuryRead article
FASEB journal : official publication of the Federation of American Societies for Experimental Biology
INPP4B protects from metabolic syndrome and associated disordersRead article
Pyrazole Agonist of the Apelin Receptor Improves Symptoms of Metabolic Syndrome in MiceRead article
Journal of medicinal chemistry