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Colorimetric and turbidimetric analysis of endotoxins using the absorbance detection mode
Torn between a colorimetric or turbidimetric LAL assay? Whichever path you take, BMG LABTECH’s absorbance readers deliver dependable results every time.
CLARIOstar Plus
アッセイ開発用の最も柔軟なプレートリーダー
CLARIOstar® Plusは、当社で最も柔軟性の高いマルチモードプレートリーダーです。特許取得済みのLVF Monochromators™に加え、フィルターおよびスペクトロメーターを搭載しています。
CLARIOstar Plusが新しくなりました。モノクロメーター式リーダーとして他の追随を許さない高感度を実現してきたCLARIOstar Plusが、新たに開発された高性能ダイナミックレンジ技術により、理想的なアッセイ構築用プレートリーダーへと進化しています。
評価
Excellent product
Very user friendly device with multimodal assay options that are easy to programme. Excellent...
4.7/5.0 (124)
CLARIOstar Plus
CLARIOstar Plusは、LVF式モノクロメーター、高感度フィルターそして高速フルスペクトロメーターを搭載したマルチモードマイクロプレートリーダーです。高性能なダイナミックレンジ技術とオートフォーカス機能により、測定条件の最適化がこれまで以上に容易になります。
O2/CO2ガスコントロール、温度制御、独自の各種シェイキング、高解像度ウェルスキャン、内蔵型試薬ディスペンサを備え、CLARIOstar Plusはライブセルアッセイに最適な測定環境を提供します。
CLARIOstar Plusには、BMG LABTECHの新しいマイクロプレートリーダー制御インターフェース「Voyager」が搭載されています。複雑さを抑えつつ柔軟性を最大化する設計により、Voyagerはこれまで以上に直感的なデータ取得を可能にします。
CLARIOstar Plusは研究用途にのみご使用いただけます。
特徴
- 高感度を実現する革新的なモノクロメーター
- 波長選択およびバンド幅を自由に設定可能
- 吸光度・蛍光・発光におけるスペクトルスキャン
- 大幅に拡張されたダイナミックレンジから最適ポイントを自動設定
- 高速フルプレートオートフォーカス機能
- ライブセルアッセイに対応した専用機能
- 超高速データ処理（100ポイント／秒）
- Alphaテクノロジー専用レーザー光源を搭載
信頼され、支持されています：
CLARIOstar Plus
当社の技術
Measurement of a reconstituted circadian clock using fluorescence polarization
Unlocking Nature’s Timekeepers. Learn how to read the internal clock of organisms and how to decode life’s 24-hour rhythm at the molecular level using a microplate reader.
Multiplexing the production of two G protein-coupled receptor second messengers using spectrally resolved fluorescent dyes
Streamline your workflow and double your insights: multiplexing cellular signal responses of multiple second messengers in a single measurement saves valuable time and conserves reagents.
Detection of bacterial endotoxins using the PYROSTAR™ ES-F/Plate LAL assay
Perform the PYROSTAR ES-F/Plate LAL test your way— BMG LABTECH readers support both turbidimetric and nephelometric evaluation for maximum flexibility.
Fast and highly sensitive pyrogen detection with the LumiMAT™ assay performed on BMG LABTECH microplate readers
Are you a fan of the monocyte activation test for endotoxin tests, but would like to speed up the testing process? Read here how the LumiMAT test and BMG LABTECH readers can support you.
Determination of Minimum Inhibitory Concentration (MIC) of antibiotics using OD600
Do you want to make your MIC measurements more efficient and automated? Find out here how BMG LABTECH microplate readers can support you in this task.
Detection Modes
Fluorescence intensity (incl. FRET)
Fluorescence polarization
AlphaScreen®/AlphaLISA®/AlphaPlex™
Luminescence (flash and glow) - incl. BRET
Time-resolved fluorescence
TR-FRET
UV/vis absorbance spectra
Measurement Modes
Top and bottom reading
Endpoint and kinetic
Sequential multi-excitation
Sequential multi-emission
Spectral scanning (fluorescence, luminescence, absorbance)
Ratiometric measurements
Well scanning
Microplate Formats
6- to 1536-well plates, user-definable
LVis Plate with 16 low volume microspots (2 μL)
Microplate Carrier
Robot compatible
Light Sources
High energy xenon flash lamp
Dedicated laser for AlphaScreen®/AlphaLISA®/AlphaPlex™
Detectors
Low-noise photomultiplier tube
Red-shifted photomultiplier tube
CCD spectrometer
Wavelength Selection
Dual Linear Variable Filter (LVF) Monochromators™
Linear Variable Dichroic Mirror
Separates ex & em LVF Monochromators
Optical filters:
Ex and em slides hold 4 filters each
LVF Monochromators + optical filters
Use one for ex and the other for em
UV/Vis absorbance spectrometer:
Full spectra or 8 distinct wavelengths in < 1 sec/well
Spectral Range
Filters
FI, FP, TRF: 240 - 740 nm or 240 - 900 nm (red-shifted PMT)
LUM: 240 - 740 nm
LVF Monochromators™
FI: 320 - 740 nm or 320 - 840 nm (red-shifted PMT)
LUM: 320 - 740 nm
Linear Variable Dichroic
340 - 740 nm or 340 - 760 nm (red-shifted PMT)
Spectrometer
ABS: 220 - 1000 nm; wavelength precision: ≤ 0.5 nm
Sensitivity
FI filters
(top)
< 0.15 pM (< 3 amol/well FITC, 384sv, 20 μL).
FI filters
(bottom)
< 1.0 pM (< 50 amol/well FITC, 384g, 50 μL).
FI monochromator
(top)
< 0.35 pM (< 7 amol/well FITC, 384sv, 20 μL).
FI monochromator
(bottom)
< 3.0 pM (< 150 amol/well FITC, 384g, 50 μL).
Dynamic Range: 8 decades in a single measurement.
FP
< 0.5 mP SD at 1 nM FITC (384sv, 20 μL).
HTRF®
(black and white microplates)
Reader Control Kit (Eu) after 18h (384sv, 20 μL).
Delta F > 880% (High Calibrator).
Delta F > 30% (Low Calibrator).
TRF
< 20 fM europium, 384, 80 μL (<1.6 amol/well).
LUM
< 0.4 pM (< 8 amol/well ATP, 384sv, 20 μL).
Dynamic range: 8 decades in a single measurement.
AlphaScreen® with laser.
< 5 pM (< 100 amol/well P-Tyr-100, 384sv, 20 μL).
ABS with spectrometer.
Selectable spectral resolution: 1, 2, 5, and 10 nm.
OD range: 0 to 4 OD; photometric resolution: 0.001 OD.
Accuracy: < 1% at 2 OD.
Precision: < 0.5% at 1 OD and < 0.8% at 2 OD.
Linearity: ≤ 0.8% at 2.0 OD.
Read Times
Flying mode (1 flash)
8 s (96), 15 s (384), 28 s (1536)
10 flashes
19 s (96), 57 s (384), 3 min 4 s (1536)
Shaking
Linear, orbital, and double-orbital with user-definable time and speed
Incubation
+3 °C above ambient up to 45 °C or 65 °C
The upper heating plate of the incubation chamber operates at 0.5 °C more than the lower plate. This prevents condensation build-up on the lid or sealer
Software
Multi-user reader control (SMART Control and Voyager) and data analysis software (MARS). Voyager is compatible with CLARIOstar Plus readers from serial number 430-4001.
FDA 21 CFR Part 11 compliance available with SMART Control and MARS.
Integrated fluorophore library.
Dimensions
Width: 45 cm, depth: 51 cm, height: 40 cm; weight: 32 kg
Optical Filters
Excitation and emission slides for 4 filters each
Optical Path Guides
Top and bottom: free-air optical light path guided by motor-driven mirrors and dichroics
Z-Adjustment
Automatic focal height adjustment (0.1 mm resolution)
Reagent Injection
Up to 2 built-in reagent injectors
Individual injection volumes for each well: 3 to 500 µL (optionally up to 2 mL)
Variable injection speed up to 420 µL/s
Reagent back flushing
Integrated Barcode Reader
Up to two integrated barcode readers
Power Requirements
- 100 or 115 or 230 V, 50/60 Hz, auto ranging
Power Consumption
- Normal operating conditions: 50 W
- Idle State: 20W
- Switched off: 0W
- max. 300 VA
Optional Accessories
LVis Plate
Sample capacity: sixteen separate microdrop wells for 2 µL samples; one standard cuvette position for up to 1 mL samples
Quality Control Internal Standards (optional): four NIST traceable optical density filters (approximate values of 0.1, 0.3, 0.6 and 1.0 OD); one holmium oxide filter for wavelength accuracy
Dimensions: conforms to SBS standards for microplates
THERMOstar
Microplate incubator and shaker
Atmospheric Control Unit (ACU)
Actively regulates O2 and CO2 - 0.1-20%
Stacker
Magazines for up to 50 plates - continuous loading feature. Only compatible with SMART Control.
Optical Filters
Excitation and emission filters
Upgrades
The CLARIOstar Plus can include all or any combination of features listed below at purchase. Upgrading with additional features is possible at any time
Please contact your local representative for more details or a quote
*
LOD = 3 x SD (20 blanks) / slope (6 pt std curve)
AlphaScreen® P-Tyr-100 assay kit, PerkinElmer, #6760620C
Microplates: white for LUM, AlphaScreen®, TRF; black for FI, FP; clear for ABS
96 = 96-well microplates
384sv = 384-well small volume microplates
384g = 384-well glass bottom microplates
1536 = 1536-well microplates
The CLARIOstar Plus is for research use only.
高感度対応アクセサリー CLARIOstar Plus
THERMOstar
Intelligent microplate incubation
Microplate Stacker
Microplate handling system
LVis Plate
Low-volume measurements
Filters
Filters for a wide range of applications
Evaluation Plate
Functional Microplate Reader Validation
Atmospheric Control Unit
Enhanced cell-based assays