Absorbance plate reader with cuvette port
BMG LABTECH’s innovative absorbance microplate reader has the flexibility to perform assays quickly and easily in both microplates or via the built-in cuvette port. This spectrometer-based absorbance microplate reader captures a full UV/visible spectrum in less than 1 sec/well. Its speed, simple push button operation, and capacity to store individual assay protocols make the SPECTROstar® Nano the leading microplate reader for absorbance measurements.
The SPECTROstar Nano is equipped with BMG LABTECH’s ultra-fast UV/vis spectrometer, which allows users to measure full-spectrum absorbance (220 – 1000 nm) in less than 1 second per well. It is the ideal instrument to read all absorbance assays in a microplate or cuvette – with many common assay protocols predefined and available at the click of the mouse.
"One thing to point out is the wonderful ability to run a complete scan and then go in afterwards and select the peak areas and set wavelengths for analysis. We would not have seen the unique spectral shift in our dye-end point water hardness method using our older single wavelength instrument. The SPECTROstar Nano is making work and life easier, more unique and more interesting for us."
Gary Spedding, Brewing and Distilling Analytical Services, USA
Detection of plant-synthesized nanoparticles and their antibacterial capacitySalem W. and Schild S., University of Graz , Institute of Molecular Biosciences , BioTechMed-Graz , Austria, 03/2017
Metallic nanoparticles became subject of intensive research because of their potential antibiotic properties. Nanoparticles such as silver, gold or zinc oxide particles are easily and cost-effectively synthesized by blending metal salts with plant extracts that reduce the metal. Different extracts, varying in the plant or the part of the plant used for the extract, are currently investigated in regard to their capacity to form nanoparticles and their antimicrobial efficacy. The formation of nanoparticles can be verified by UV-Vis spectroscopy due to surface plasmon resonance of the particles that lead to a characteristic spectrum defined by the underlying metal and particle size. Subsequent analysis of nanoparticles on microbial growth is typically tested by methods based on absorbance changes.
Here, we present how the spectrometer-based BMG LABTECH instruments are used to quickly confirm Ag and ZnO nanoparticle formation and their inhibitory effect on the diarrhea-causing bacteria Vibrio cholerae and enterotoxic Escherichia coli (ETEC).
Absorbance-based methods for protein quantification on BMG LABTECH instrumentsAndrea Krumm, BMG LABTECH, 09/2016
Measuring the protein concentration of liquid samples is a routine analysis in many life science laboratories. Accurate quantification is often a critical step for subsequent analyses such as protein characterization or western blots. Absorbance-based methods are well-established, easy to handle and cheap. They can be performed in microplates, allowing for high sample numbers processed at a time and low reagent volumes.
Spectrometer-based BMG LABTECH readers capture absorbance spectra or absorbance at discrete wavelengths from 220-1000 nm in less than a second per well and therefore can easily read these assays. The most common methods absorbance at 280 nm, Bradford, Bicin-Choninic Acid (BCA) and Lowry assay are presented here.
ProteaseTag active NE immunoassay: a rapid test to quantify neutrophil elastase levels in patientsOliver Carney (1) , Kelly Moffitt (2) , Charlene Robb (2), 1) BMG LABTECH , 2) ProAxsis, 02/2016
The neutrophil elastase is a biomarker of respiratory diseases such as cystic fibrosis (CF) or chronic obstructive pulmonary disease (COPD). A simple, rapid and accurate test for neutrophil elastase levels in patient samples would be a significant medical benefit. Established methods do not specifically verify the presence of the enzyme leading to a high value of false positives. In this application note we show how to use the ProteaseTag™ Active Neutrophil Elastase Immunoassay from the company ProAxsis which is based in Northern Ireland.
The assay uses tags to specifically bind the enzyme of interest. Chromogenic detection is performed with the CLARIOstar® microplate reader which analysis the results and return qualified data automatically.
Protease levels can be determined in sputum sol, bronchoalveolar lavage, wound exudate or serum free cell culture.
Authentication and quality testing of distilled spirits using the SPECTROstar NanoGary Spedding (1) , Carl Peters (2), 1) Brewing and Distilling Analytical Services LLC , 2) BMG LABTECH, 01/2015
Alcoholic beverages that are produced by distillation are called distilled beverages. Some known distilled beverages include vodka, gin, tequila, rum, and whisky. Beer, wine, and cider are excluded from this category as these are fermented but not distilled.
The distilled beverages industry is expanding, indicated by the constant release of new products. On the other hand, counterfeiters as well as cases of adulteration and dilution of distilled spirits and liqueurs are also on the increase. It would be of great advantage to find an easy way to test distilled spirits for authentication.
One possible method is to take UV-Vis absorbance spectra from the liquid samples. Recently, it was shown in literature that these spectra work as brand "fingerprints". There is, for example, a clear difference between a whiskey that has aged in wood compared to an unaged whiskey.
A convenient way to measure UV-Vis absorbance spectra and to perform a quality control check is achieved by using the SPECTROstar® Nano. This microplate and cuvette reader offers rapid and efficient spectral analysis measurements. The associated MARS data analysis software stores all spectral data in a way that the creation of specific spirit fingerprint libraries is possible.
Label-free SoPRano Gold Nano-Rod (GNR) assays on a spectrometer-based microplate readerEJ Dell , (1) , Meike Roskamp (2) , Frederik Van de Velde (2), 1) BMG LABTECH , 2) PharmaDiagnostics, 12/2014
The Label-Free SoPRanoTM Gold Nano-Rod assay depends on absorbance shifts that are induced by protein binding due to localized surface plasmon resonance (LSPR). A protein is coupled to a gold rod and if interaction of the coupled protein with another protein occurs, a red shift of the absorbance maximum can be detected that is derived from a change in the local refractive index.
The method requires acquisition of absorbance spectra which is provided by all spectrometer-based microplate readers by BMG LABTECH. They were able to detect the slight differences in absorbance of human serum albumin coupled gold-nano-rods and the hSA-rods bound by anti-hSA antibodies. The assay allowed for detection of different concentrations of the antibody and for kinetic measurements revealing binding constants.
In contrast to other LSPR-based systems, SoPRanoTM requires no specialized and costly instrumentation. Instead, a microplate reader acquires the absorbance spectrum which is done by a spectrometer-based BMG LABTECH instrument in less than a second.