Monitoring of microbial growth curves by laser nephelometry

Sven Brand, Christian Rückert University of Bielefeld 07/2004

Nephelometry is a direct method of measuring light scattered by particles suspended in solution at right angles to the beam, or preferably, at a forward angle. In dilute solutions, where absorption and reflection are minimal, the intensity of the scattered light is a function of the concentration of scattering particles such as microbes.


In this application note, the only laser-based microplate nephelometer was used to monitor growth of the Corynebacterium glutamicum. Relative nephelometric units detected by the NEPHELOstar® Plus were compared to OD600 absorbance measurements and revealed that both density measurements correlated well, with nephelometry being the more sensitive method.

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