The direct measurement of insulin is necessary for studying the mechanisms of glucose stimulated insulin release. Together with Cisbio, an HTRF®-based insulin detection assay has been developed. The assay provides quick and cost-effective results and proves suitable for HTS purposes.
Secreted insulin in cells or supernatant is transferred to a new microplate, antibodies coupled to HTRF chemistry are added. After a short incubation time the signals are detected. HTRF measurements were performed with the PHERAstar® FS microplate reader which is designed to optimize HTRF detection.
The assay is able to detect insulin from a variety of species. Examples are given for insulin from humans, rodent, pigs or cows. The system is further suitable to detect the effect of modulators on insulin secretion.