Cell biology

Discover how cell-based microplate assays advance your research: from viability to cell death mechanisms, from glycolysis to respiration, from lytic assays to live cell assays, from one to multiple assays.

Cell-based studies provide information about basic cellular processes. These include cell division, cell energy balance, control mechanisms, cell death regulation, differentiation or pathology. Cell-based methods are not only used for research: toxicity tests replace animal experiments and identify toxic substances in vitro. In addition, cell cultures have become important production organisms for medicines. For example, they produce insulin or monoclonal antibodies used as cancer therapeutics.


Modern cell biology analyses not only measure at a single time point but monitor biological changes over days in real time. To this end, the measurement chamber of a microplate reader needs controlled temperature and gas conditions. BMG LABTECH´s Atmospheric Control Unit regulates CO2 to stabilize the pH in carbonate buffered cell cultures. Additionally, O2 can be set to physiological conditions, which for mammalian cultures is typically below ambient concentrations. BMG LABTECH microplate readers further correct for non-homogenous distribution of a cell layer as they measure several points across the well instead of only reading in the center. To avoid biased results due to a undetected mycoplasma contamination, BMG LABTECH readers also offer the possibility of detecting existing contamination.


Setting up a cell biology experiment takes time. Therefore, novel assay solutions measure several parameters at once. The combination of different fluorescent, luminescent and chromogenic assays in one well results in multiple readouts. With a flexible microplate reader, all assays can be measured with only one instrument.

Resources

Search our Resources section for information about specific applications, literature citations, videos, blog articles and many other publications. Many of the resources provided are associated with current and previous instrument models and versions.

    Ischemia-reperfusion proof-of-concept as example for Coronary Heart Diseases
    August 09, 2019

    Mimicking the in vivo environment of coronary heart disease

    Coronary heart disease (CHD) is a complex condition that affects millions of people worldwide. There are many pathways and processes involved in the pathogenesis of this disease, and attempting to mimic these interactions in laboratory environments can prove troublesome. This blog article explains how ischemia and reperfusion models can be used to examine the complications of CHD and discover potential drug targets.

    Read more
    • Mitochondrial metabolism supports resistance to IDH mutant inhibitors in acute myeloid leukemia

      Stuani, L;Sabatier, M;Saland, E;Cognet, G;Poupin, N;Bosc, C;Castelli, FA;Gales, L;Turtoi, E;Montersino, C;Farge, T;Boet, E;Broin, N;Larrue, C;Baran, N;Cissé, MY;Conti, M;Loric, S;Kaoma, T;Hucteau, A;Zavoriti, A;Sahal, A;Mouchel, PL;Gotanègre, M;Cassan, C;[2021]

      The Journal of experimental medicine

      Read article
    • Selective drug combination vulnerabilities in STAT3- and TP53-mutant malignant NK cells

      Parri, E;Kuusanmäki, H;Bulanova, D;Mustjoki, S;Wennerberg, K;[2021]

      Blood advances

      Read article
    • Dimethyloxallyl glycine/nanosilicates-loaded osteogenic/angiogenic difunctional fibrous structure for functional periodontal tissue regeneration

      Shang, L;Liu, Z;Ma, B;Shao, J;Wang, B;Ma, C;Ge, S;[2021]

      Bioactive Materials

      Read article
    • Gut vascular barrier impairment leads to intestinal bacteria dissemination and colorectal cancer metastasis to liver

      Bertocchi, A;Carloni, S;Ravenda, P;Bertalot, G;Spadoni, I;Lo Cascio, A;Gandini, S;Lizier, M;Braga, D;Asnicar, F;Segata, N;Klaver, C;Brescia, P;Rossi, E;Anselmo, A;Guglietta, S;Maroli, A;Spaggiari, P;Tarazona, N;Cervantes, A;Marsoni, S;Lazzari, L;Jodice, M[2021]

      Cancer Cell

      Read article
    • Silver nanoparticle induced toxicity and cell death mechanisms in embryonic zebrafish cells

      Quevedo, AC;Lynch, I;Valsami-Jones, E;[2021]

      Nanoscale

      Read article