Viruses are equally a threat to plants, bacteria, animals, and humans. They use their hosts to reproduce and can thereby damage them. This can lead, for example, to crop or farm animal losses and pandemics. On the other hand, viruses serve as tools for genetic engineering and the targeted modification of genomes.
Modern virology characterises viruses molecularly and functionally and uses this information to develop diagnostic tests, antiviral drugs and vaccines. Traditionally, virology largely relied on microscopic methods. Nowadays, microplate-based assays increase throughput and enable the measurement of replication, virus neutralization, binding of molecules to viral particles and much more.
Virus assays range from simple ELISA assays for measuring antibody titer to live-cell assays to measure replication. The variety of virus assays in combination with the need for cell-based methods requires a flexible microplate reader.
The CLARIOstar®Plus microplate reader offers this flexibility. It is a modular multi-mode reader that can be equipped with fluorescence, luminescence, absorbance and advanced detection modes. With its Atmospheric Control Unit, it is further optimized for live-cell assays as it creates the optimal environment for long-term cell-based experiments. The CLARIOstar Plus can be equipped with a red-shifted PMT for increased sensitivity with fluorophores emitting in the red range of light. These are often used in cell assays to avoid autofluorescence.
The PHERAstar FSX multi-mode microplate reader is the ideal platform for screening departments, where potential anti-viral compounds have to be detected quickly and efficiently in high throughput. In addition, it can quickly and effortlessly measure all FRET, TR-FRET and fluorescence polarization dual emission assays. These are often used in binding/interaction assays for anti-viral compound screens.
Browse our Resources section for information about specific applications, literature citations, videos, blog articles and many other publications. Many of the resources provided are associated with current and previous instrument models and versions.
Fluorescence polarization-based RNA synthesis assayStefan Reich , Stephen Cusack, European Molecular Biology Laboratory, 10/2018
Molecular beacon based helicase assaysAlicia M. Hanson (1) , David N. Frick (1) , Carl Peters (2), (1) University of Wisconsin-Milwaukee , (2) BMG LABTECH, 06/2013
Dual luciferase assay to assess the replication of the hepatitis C virus subgenomic repliconSarah Gretton , Mark Harris, Faculty of Biologial Sciences , University of Leeds, 09/2008
Vaccine Development: past, present and future
High Blood Pressure medication, G-protein coupled receptors and viruses
APOBEC3C Tandem Domain Proteins Create Super Restriction Factors against HIV-1Read article
Investigating the Effect of Encapsulation Processing Parameters on the Viability of Therapeutic Viruses in ElectrosprayingRead article
Structural basis for Zika envelope domain III recognition by a germline version of a recurrent neutralizing antibodyRead article
Proc. Natl. Acad. Sci. U.S.A.
N-glycosylation of infectious bronchitis virus M41 spike determines receptor specificityRead article
J. Gen. Virol.
A combination of two human monoclonal antibodies limits fetal damage by Zika virus in macaquesRead article
Proc. Natl. Acad. Sci. U.S.A.