Highly sensitive NanoBRET measurements using the LVF monochromator or filter-based readers
With the NanoLuc® luciferase, Promega has created the next generation of BRET assays. The NanoLuc® enzyme is smaller in size than other luciferases. At the same time its emission is brighter by several factors. These properties make the NanoLuc® the perfect BRET donor. As BRET acceptor, a fluorescently labeled HaloTag® fusion protein is used that emits light in the red range (>610 nm).
The NanoBRET assay technology is intended for studying protein-protein interactions in living cells. One example is the Frb binding to FKBP in response to an inhibitor (Rapamycin). This measurement was performed on a CLARIOstar® microplate reader. With its linear variable filter (LVF) monochromator™ the CLARIOstar was able to match the two NanoBRET wavelengths without special filters. For all other BMG LABTECH instruments filters optimized for NanoBRET are also available.
The boxes below lead on to NanoBRET related products and application notes.