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Colorimetric and turbidimetric analysis of endotoxins using the absorbance detection mode
Torn between a colorimetric or turbidimetric LAL assay? Whichever path you take, BMG LABTECH’s absorbance readers deliver dependable results every time.
CLARIOstar Plus
분석법 개발을 위한 가장 유연한 플레이트 리더
CLARIOstar® Plus는 최적의 결과를 획득하는 사용하기 편리한 가장 유연한 다중 모드 플레이트 리더로, 당사의 독자적인 특허 기술인 LVF Monochromators™, 필터 및 분광계가 장착되어 있습니다. 최고 수준의 감도와 결합된 유연성, 그리고 새롭게 개발한 향상된 동적 범위(Enhanced Dynamic Range) 기술은 CLARIOstar Plus가 어세이 개발을 위한 이상적인 리더임을 보여줍니다.
평가
Excellent product
Very user friendly device with multimodal assay options that are easy to programme. Excellent...
4.7/5.0 (124)
CLARIOstar Plus
CLARIOstar Plus는 고급 LVF Monochromators™, 고감도 필터 및 초고속 UV / vis 분광기를 갖춘 멀티 모드 마이크로 플레이트 리더입니다. 향상된 동적 범위(Enhanced Dynamic Range)와 오토포커스 기술 덕분에 수동 설정이 불필요해지고 검출 최적화가 매우 쉬워졌습니다.
대기 조절 장치(ACU), 온도 배양, 멀티플 진탕(shaking), 웰(well) 스캔, 시약 주입기를 갖추고 있기때문에 CLARIOstar Plus는 모든 종류의 라이브 셀 기반 시험에 이상적인 플랫폼입니다.
CLARIOstar Plus는 이제 BMG LABTECH의 새로운 마이크로플레이트 리더 제어 인터페이스인 Voyager와 함께 제공됩니다. 복잡성을 줄이면서 유연성을 극대화하도록 설계된 Voyager는 데이터 수집을 그 어느 때보다 직관적으로 만듭니다.
CLARIOstar Plus는 연구용으로만 사용됩니다.
주요 기능
- 향상된 감도를위한 혁신적인 모노 크로미터
- 완전히 유연한 파장 및 대역폭
- AS, FI 및 LUM의 스펙트럼 스캔
- 수동 개입이 전혀 없는 최대의 동적 범위
- 빠른 풀 플레이트 오토포커스
- 라이브 셀 기반 분석을위한 전용 기능
- 초고속 데이터 샘플링(1초당 100개 측정)
- 알파 기술 전용 레이저
신뢰와 사랑을 받고 있습니다:
선택 액세서리
- Atmospheric Control Unit: O₂ 및 CO₂ 가스 조절 모듈
- THERMOstar: 마이크로플레이트 인큐베이터 및 오비탈 셰이커
- Microplate Stacker: 마이크로플레이트 처리 시스템
- LVis Plate: 저용량 측정 및 성능 테스트용 플레이트
- Filters: 다양한 응용 분야용 필터 제공
- Evaluation Plate: 광학 기능 검증용 플레이트
CLARIOstar Plus
우리의 기술
Measurement of a reconstituted circadian clock using fluorescence polarization
Unlocking Nature’s Timekeepers. Learn how to read the internal clock of organisms and how to decode life’s 24-hour rhythm at the molecular level using a microplate reader.
Multiplexing the production of two G protein-coupled receptor second messengers using spectrally resolved fluorescent dyes
Streamline your workflow and double your insights: multiplexing cellular signal responses of multiple second messengers in a single measurement saves valuable time and conserves reagents.
Detection of bacterial endotoxins using the PYROSTAR™ ES-F/Plate LAL assay
Perform the PYROSTAR ES-F/Plate LAL test your way— BMG LABTECH readers support both turbidimetric and nephelometric evaluation for maximum flexibility.
Fast and highly sensitive pyrogen detection with the LumiMAT™ assay performed on BMG LABTECH microplate readers
Are you a fan of the monocyte activation test for endotoxin tests, but would like to speed up the testing process? Read here how the LumiMAT test and BMG LABTECH readers can support you.
Determination of Minimum Inhibitory Concentration (MIC) of antibiotics using OD600
Do you want to make your MIC measurements more efficient and automated? Find out here how BMG LABTECH microplate readers can support you in this task.
Detection Modes
Fluorescence intensity (incl. FRET)
Fluorescence polarization
AlphaScreen®/AlphaLISA®/AlphaPlex™
Luminescence (flash and glow) - incl. BRET
Time-resolved fluorescence
TR-FRET
UV/vis absorbance spectra
Measurement Modes
Top and bottom reading
Endpoint and kinetic
Sequential multi-excitation
Sequential multi-emission
Spectral scanning (fluorescence, luminescence, absorbance)
Ratiometric measurements
Well scanning
Microplate Formats
6- to 1536-well plates, user-definable
LVis Plate with 16 low volume microspots (2 μL)
Microplate Carrier
Robot compatible
Light Sources
High energy xenon flash lamp
Dedicated laser for AlphaScreen®/AlphaLISA®/AlphaPlex™
Detectors
Low-noise photomultiplier tube
Red-shifted photomultiplier tube
CCD spectrometer
Wavelength Selection
Dual Linear Variable Filter (LVF) Monochromators™
Linear Variable Dichroic Mirror
Separates ex & em LVF Monochromators
Optical filters:
Ex and em slides hold 4 filters each
LVF Monochromators + optical filters
Use one for ex and the other for em
UV/Vis absorbance spectrometer:
Full spectra or 8 distinct wavelengths in < 1 sec/well
Spectral Range
Filters
FI, FP, TRF: 240 - 740 nm or 240 - 900 nm (red-shifted PMT)
LUM: 240 - 740 nm
LVF Monochromators™
FI: 320 - 740 nm or 320 - 840 nm (red-shifted PMT)
LUM: 320 - 740 nm
Linear Variable Dichroic
340 - 740 nm or 340 - 760 nm (red-shifted PMT)
Spectrometer
ABS: 220 - 1000 nm; wavelength precision: ≤ 0.5 nm
Sensitivity
FI filters
(top)
< 0.15 pM (< 3 amol/well FITC, 384sv, 20 μL).
FI filters
(bottom)
< 1.0 pM (< 50 amol/well FITC, 384g, 50 μL).
FI monochromator
(top)
< 0.35 pM (< 7 amol/well FITC, 384sv, 20 μL).
FI monochromator
(bottom)
< 3.0 pM (< 150 amol/well FITC, 384g, 50 μL).
Dynamic Range: 8 decades in a single measurement.
FP
< 0.5 mP SD at 1 nM FITC (384sv, 20 μL).
HTRF®
(black and white microplates)
Reader Control Kit (Eu) after 18h (384sv, 20 μL).
Delta F > 880% (High Calibrator).
Delta F > 30% (Low Calibrator).
TRF
< 20 fM europium, 384, 80 μL (<1.6 amol/well).
LUM
< 0.4 pM (< 8 amol/well ATP, 384sv, 20 μL).
Dynamic range: 8 decades in a single measurement.
AlphaScreen® with laser.
< 5 pM (< 100 amol/well P-Tyr-100, 384sv, 20 μL).
ABS with spectrometer.
Selectable spectral resolution: 1, 2, 5, and 10 nm.
OD range: 0 to 4 OD; photometric resolution: 0.001 OD.
Accuracy: < 1% at 2 OD.
Precision: < 0.5% at 1 OD and < 0.8% at 2 OD.
Linearity: ≤ 0.8% at 2.0 OD.
Read Times
Flying mode (1 flash)
8 s (96), 15 s (384), 28 s (1536)
10 flashes
19 s (96), 57 s (384), 3 min 4 s (1536)
Shaking
Linear, orbital, and double-orbital with user-definable time and speed
Incubation
+3 °C above ambient up to 45 °C or 65 °C
The upper heating plate of the incubation chamber operates at 0.5 °C more than the lower plate. This prevents condensation build-up on the lid or sealer
Software
Multi-user reader control (SMART Control and Voyager) and data analysis software (MARS). Voyager is compatible with CLARIOstar Plus readers from serial number 430-4001.
FDA 21 CFR Part 11 compliance available with SMART Control and MARS.
Integrated fluorophore library.
Dimensions
Width: 45 cm, depth: 51 cm, height: 40 cm; weight: 32 kg
Optical Filters
Excitation and emission slides for 4 filters each
Optical Path Guides
Top and bottom: free-air optical light path guided by motor-driven mirrors and dichroics
Z-Adjustment
Automatic focal height adjustment (0.1 mm resolution)
Reagent Injection
Up to 2 built-in reagent injectors
Individual injection volumes for each well: 3 to 500 µL (optionally up to 2 mL)
Variable injection speed up to 420 µL/s
Reagent back flushing
Integrated Barcode Reader
Up to two integrated barcode readers
Power Requirements
- 100 or 115 or 230 V, 50/60 Hz, auto ranging
Power Consumption
- Normal operating conditions: 50 W
- Idle State: 20W
- Switched off: 0W
- max. 300 VA
Optional Accessories
LVis Plate
Sample capacity: sixteen separate microdrop wells for 2 µL samples; one standard cuvette position for up to 1 mL samples
Quality Control Internal Standards (optional): four NIST traceable optical density filters (approximate values of 0.1, 0.3, 0.6 and 1.0 OD); one holmium oxide filter for wavelength accuracy
Dimensions: conforms to SBS standards for microplates
THERMOstar
Microplate incubator and shaker
Atmospheric Control Unit (ACU)
Actively regulates O2 and CO2 - 0.1-20%
Stacker
Magazines for up to 50 plates - continuous loading feature. Only compatible with SMART Control.
Optical Filters
Excitation and emission filters
Upgrades
The CLARIOstar Plus can include all or any combination of features listed below at purchase. Upgrading with additional features is possible at any time
Please contact your local representative for more details or a quote
*
LOD = 3 x SD (20 blanks) / slope (6 pt std curve)
AlphaScreen® P-Tyr-100 assay kit, PerkinElmer, #6760620C
Microplates: white for LUM, AlphaScreen®, TRF; black for FI, FP; clear for ABS
96 = 96-well microplates
384sv = 384-well small volume microplates
384g = 384-well glass bottom microplates
1536 = 1536-well microplates
The CLARIOstar Plus is for research use only.
For more performance: Accessories for CLARIOstar Plus
THERMOstar
Intelligent microplate incubation
Microplate Stacker
Microplate handling system
LVis Plate
Low-volume measurements
Filters
Filters for a wide range of applications
Evaluation Plate
Functional Microplate Reader Validation
Atmospheric Control Unit
Enhanced cell-based assays