CLARIOstar Plus

Fluorescence intensity (incl. FRET)
Fluorescence polarization
AlphaScreen^®/AlphaLISA^®/AlphaPlex™
Luminescence (flash and glow) - incl. BRET
Time-resolved fluorescence
TR-FRET
UV/vis absorbance spectra

Top and bottom reading
Endpoint and kinetic
Sequential multi-excitation
Sequential multi-emission
Spectral scanning (fluorescence, luminescence, absorbance)
Ratiometric measurements
Well scanning

6- to 1536-well plates, user-definable
LVis Plate with 16 low volume microspots (2 μL)

Robot compatible

High energy xenon flash lamp
Dedicated laser for AlphaScreen^®/AlphaLISA^®/AlphaPlex™

Low-noise photomultiplier tube
Red-shifted photomultiplier tube
CCD spectrometer

Dual Linear Variable Filter (LVF) Monochromators™
Linear Variable Dichroic Mirror
Separates ex & em LVF Monochromators

Optical filters:
Ex and em slides hold 4 filters each
LVF Monochromators + optical filters
Use one for ex and the other for em
UV/Vis absorbance spectrometer:
Full spectra or 8 distinct wavelengths in < 1 sec/well

Filters
FI, FP, TRF: 240 - 740 nm or 240 - 900 nm (red-shifted PMT)
LUM: 240 - 740 nm

LVF Monochromators™
FI: 320 - 740 nm or 320 - 840 nm (red-shifted PMT)
LUM: 320 - 740 nm

Linear Variable Dichroic
340 - 740 nm or 340 - 760 nm (red-shifted PMT)

Spectrometer
ABS: 220 - 1000 nm; wavelength precision: ≤ 0.5 nm

FI filters
(top)
< 0.15 pM (< 3 amol/well FITC, 384sv, 20 μL).

FI filters
(bottom)
< 1.0 pM (< 50 amol/well FITC, 384g, 50 μL).

FI monochromator
(top)
< 0.35 pM (< 7 amol/well FITC, 384sv, 20 μL).

FI monochromator
(bottom)
< 3.0 pM (< 150 amol/well FITC, 384g, 50 μL).
Dynamic Range: 8 decades in a single measurement.

FP
< 0.5 mP SD at 1 nM FITC (384sv, 20 μL).

HTRF^®
(black and white microplates)
Reader Control Kit (Eu) after 18h (384sv, 20 μL).
Delta F > 880% (High Calibrator).
Delta F > 30% (Low Calibrator).

TRF
< 20 fM europium, 384, 80 μL (<1.6 amol/well).

LUM
< 0.4 pM (< 8 amol/well ATP, 384sv, 20 μL).
Dynamic range: 8 decades in a single measurement.

AlphaScreen^® with laser.
< 5 pM (< 100 amol/well P-Tyr-100, 384sv, 20 μL).

ABS with spectrometer.
Selectable spectral resolution: 1, 2, 5, and 10 nm.
OD range: 0 to 4 OD; photometric resolution: 0.001 OD.
Accuracy: < 1% at 2 OD.
Precision: < 0.5% at 1 OD and < 0.8% at 2 OD.
Linearity: ≤ 0.8% at 2.0 OD.

Flying mode (1 flash)
8 s (96), 15 s (384), 28 s (1536)

10 flashes
19 s (96), 57 s (384), 3 min 4 s (1536)

Linear, orbital, and double-orbital with user-definable time and speed

+3 °C above ambient up to 45 °C or 65 °C

The upper heating plate of the incubation chamber operates at 0.5 °C more than the lower plate. This prevents condensation build-up on the lid or sealer

Multi-user reader control (SMART Control and Voyager) and data analysis software (MARS). Voyager is compatible with CLARIOstar Plus readers from serial number 430-4001. 

FDA 21 CFR Part 11 compliance available with SMART Control and MARS.

Integrated fluorophore library.

Width: 45 cm, depth: 51 cm, height: 40 cm; weight: 32 kg

Excitation and emission slides for 4 filters each

Top and bottom: free-air optical light path guided by motor-driven mirrors and dichroics

Automatic focal height adjustment (0.1 mm resolution)

Up to 2 built-in reagent injectors
Individual injection volumes for each well: 3 to 500 µL (optionally up to 2 mL)
Variable injection speed up to 420 µL/s
Reagent back flushing

Up to two integrated barcode readers

• 100 or 115 or 230 V, 50/60 Hz, auto ranging

• Normal operating conditions: 50 W
• Idle State: 20W
• Switched off: 0W
• max. 300 VA

Sample capacity: sixteen separate microdrop wells for 2 µL samples; one standard cuvette position for up to 1 mL samples

Quality Control Internal Standards (optional): four NIST traceable optical density filters (approximate values of 0.1, 0.3, 0.6 and 1.0 OD); one holmium oxide filter for wavelength accuracy

Dimensions: conforms to SBS standards for microplates

Microplate incubator and shaker

Actively regulates O₂ and CO₂ - 0.1-20%

Magazines for up to 50 plates - continuous loading feature. Only compatible with SMART Control.

Excitation and emission filters

The CLARIOstar Plus can include all or any combination of features listed below at purchase. Upgrading with additional features is possible at any time

Please contact your local representative for more details or a quote

LOD = 3 x SD (20 blanks) / slope (6 pt std curve)
AlphaScreen^® P-Tyr-100 assay kit, PerkinElmer, #6760620C
Microplates: white for LUM, AlphaScreen^®, TRF; black for FI, FP; clear for ABS
96 = 96-well microplates
384sv = 384-well small volume microplates
384g = 384-well glass bottom microplates
1536 = 1536-well microplates

The CLARIOstar Plus is for research use only.