BellBrook Labs offers HTS assays for various enzymes. The Transcreener® ADP2 FP assay can be used to detect the activity of any kinase or ATPase. This simple ADP detecting method is universal for ADP-producing enzymes and can be used with any substrate. In this application note we show the performance of BMG LABTECH microplate readers in measuring ADP/ATP standard curves with the Transcreener® assay.
The Transcreener® fluorescence polarization assays employ an antibody against the analyte which is bound to a fluorophore labeled analyte prior to the test. An analyte produced during the reaction is unlabeled and displaces the fluorophore labeled analyte. The released fluorophore labeled molecule leads to a decrease in FP and correlates with analyte concentration.