Promega ENLITEN kit performed on a BMG LABTECH microplate reader

Ron Earp, Jarrett Cheek BMG LABTECH 05/2007

The measurement of adenosine 5’-triphosphate (ATP) is widely used to determine the amount of microorganisms in food and it can be used in the quantitation of cells in tissues and serum. The ENLITEN® kit uses L/L (luciferase/luciferin) to rapidly quantitate ATP. The luciferase depends on ATP to convert luciferin into oxyluciferin. In course of the reaction light is emitted. Hence, luminescence increases with high ATP levels.

The limits of detection (LOD) and quantitation (LOQ) of the ENLITEN® ATP quantification kit was determined on BMG LABTECH microplate readers. Limit of ATP detection was as low as 0.2 fmol/well and limit of quantitation 0.67 fmol/well. Furthermore, the readers provided linear measurements as the linear regression coefficient was >0.99 for the measurement of calibration curves.

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