Fluorescence analysis of reactive oxygen species (ROS) generated by six isolates of Aspergillus fumigatus

U.-C. Hipler (1), U. Wollina (1), D. Denning (2) (1) Department of Dermatology, Friedrich-Schiller University Jena, (2) Department of Microbiology, University of Manchester, UK 06/2004

The light emitted by the probe in the presence of ROS can be detected by all BMG LABTECH multi-mode readers. Using them with integrated reagent injectors allowed for determination of the antioxidative capacity of ascorbate and of natural plant extracts. The luminescence detection was done very fast due to the onboard injectors and with a high precision and reproducibility.

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