
Colorimetric and turbidimetric analysis of endotoxins using the absorbance detection mode
Torn between a colorimetric or turbidimetric LAL assay? Whichever path you take, BMG LABTECH’s absorbance readers deliver dependable results every time.
Quality control ensures that standards are maintained across many applied areas of the life sciences. Learn more about how consistency and accuracy are ensured for the life sciences.
For the maintenance of ordered production systems, it is essential to ensure the consistent and accurate quality of the production processes and consequently the final product. This applies in particular to products which are intended for human use such as foods, drugs, and medicinal products, or products applied to life stock or plants, since contaminants or falsely administered concentrations can lead to health issues or disease. The quality of a product is made up of various quality parameters. It is important to ensure consistent purity, composition and quantity. Microplate readers offer various solutions for determining these quality parameters.
The determination of contaminants and impurities in a sample is an important prerequisite for many downstream applications of products. Such impurities can range from undesired side products that can arise in a production process to microbial contaminations and toxins that can accumulate in a product. Ordered production systems often also include purification procedures. However, these purification processes themselves also need to be validated to ensure product purity, since such procedures can be inefficient or even introduce additional or new contaminants. Microplate reader-based methods, for example for the analysis of DNA purity or the detection of endotoxins, are the ideal tools to accomplish this task since they combine sensitivity and minimal sample volumes with the ability to achieve high sample throughput. Microplate readers are used frequently to identify mycoplasma contaminations in cell culture with assay kits like MycoAlert®.
But product purity alone isn´t the only measure for performing adequate quality control. Even if a product is pure and free of contaminants it should have a consistent composition and stability. The application note Antibody Aggregation on a Plate Reader, presents an example to test the stability of manufactured antibodies.. Drugs and biologicals, in particular have to be administered precisely to induce their effect to a targeted extent, since over-dosage can quickly lead to undesirable side effects. This principle also applies to the field of life sciences, as experimental results can only be interpreted correctly if researchers know the accurate composition and concentrations of their samples. Many methods, such as the quantification of antioxidant capacity, protein quantification, reporter assays, pyrogen measurements (including the BET test or recombinant factor C test) or ELISAs can be performed on microplate readers, offering cost-effective and accurate solutions for quality control testing.
Search our resources section for information about specific applications, literature citations, videos, blog articles and many other publications. Many of the resources provided are associated with current and previous instrument models and versions.
Torn between a colorimetric or turbidimetric LAL assay? Whichever path you take, BMG LABTECH’s absorbance readers deliver dependable results every time.
Perform the PYROSTAR ES-F/Plate LAL test your way— BMG LABTECH readers support both turbidimetric and nephelometric evaluation for maximum flexibility.
Are you a fan of the monocyte activation test for endotoxin tests, but would like to speed up the testing process? Read here how the LumiMAT test and BMG LABTECH readers can support you.
Are you looking for a method to analyse the effectiveness of your extracellular vesicles isolation ? Read here how spiking with NanoLuc®-labelled vesicles can help you to check the suitability of your method.
Do you want to streamline your endotoxin detection workflow? Check out what the combination of VANTAstar and ENDOZYME II can do for you.
Are you looking for a device to measure sample`s biotoxicity with Aliivibrio fischeri? BMG LABTECH's luminometers fulfil this task and offer additional advantages over the classic cuvette-based analysis.
Learn about the bacterial endotoxin test (BET test) and its role in ensuring the safety of pharmaceuticals, biologics and medical devices.
The monocyte activation test is a cell-based assay used to detect pyrogens. Learn more about the monocyte activation test and how microplate readers can support these crucial tests for quality control and bioanalysis.
The recombinant factor C (rFC) test is used to detect bacterial endotoxins that can cause fever and adverse events when introduced into the body. Learn about the rFC test and the advantages it offers for endotoxin detection in the life sciences.
Pyrogen tests are vital to ensure the safety of different health interventions including pharmaceuticals, medical devices and an array of biological products. This blog looks at the different types of pyrogens as well as some of the widely used pyrogen tests.
Gene reporter assays are sensitive and specific tools to study the regulation of gene expression. Learn about the different options available, their uses, and the benefits of running these types of assays on microplate readers.
You want to make sure that your cell culture is free of mycoplasma? BMG LABTECH microplate readers are the perfect measurement platform to read MycoAlert® mycoplasma detection kits.
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Absorbance plate reader with cuvette port