
Methods that answer biochemical question are as varied as the chemical processes in living organisms. They cover simple protein quantification assays such as Bradford or Lowry assay to adjust protein extracts for further analysis, as well as enzyme activity assays helping to find inhibitory and accelerating conditions. These assays benefit from BMG LABTECH´s kinetic read options, an Enhanced Dynamic Range for high intensity variations in a measurement and a suite of enzyme kinetic analysis options.
An achievement of the past 20 years has been the multitude of protein interaction assays that can be carried out in 96 to 1536 well plates. They replace lengthy or radioactive procedures and facilitate the screening for potential active substances.
BMG LABTECH microplate readers measure all these assays. They not only provide highest sensitivity and throughput, if necessary, they further assist in developing your own assays and adapting existing methods to your specific target.
Search our resources section for information about specific applications, literature citations, videos, blog articles and many other publications. Many of the resources provided are associated with current and previous instrument models and versions.
Read in this AppNote, how EDR helps to avoid signal saturation and MARS data analysis software enables the fast and simple kinetic evaluation of enzyme activity assays.
This application note highlights the use of THUNDER™ cellular kinase assays designed to enable the measurement of endogenously expressed phosphorylated proteins.
Pompe disease is associated with an inherited deficiency of alpha-glucosidase. Read here how a 4-fluorescence-based assay can be used to measure enzyme activity of the moss-produced enzyme version.
Association and dissociation rates of drugs are crucial for their effectiveness: Thanks to its speed, the PHERAstar measures the kinetics of ligand-receptor binding, even in live cells!
Learn here how the novel Enhanced Dynamic Range (EDR) technology of the CLARIOstar supersedes time consuming optimisation measurements and simplifies enzymatic reaction monitoring.
Looking for a method to study protein-nucleic acid interaction? Here protein-induced fluorescence enhancement is employed to detect protein-nucleic acid interactions in microplates.
Kinases are a large group of enzymes which are the focus of 1/3 of all drug development efforts due to their association with multiple diseases. Read more about their role and research here.
Reactive oxygen species (ROS) may have physiological as well as pathological effects. Here we explain what ROS are and how they can be measured on microplate readers.
Zhou, X;Fennema Galparsoro, D;Østergaard Madsen, A;Vetri, V;van de Weert, M;Mørck Nielsen, H;Foderà, V; [2022]
Journal of colloid and interface science 606(Pt 2) 1928-1939
Nimma, S;Gu, W;Manik, MK;Ve, T;Nanson, JD;Kobe, B; [2022]
FEBS letters
Pitman, MR;Lewis, AC;Davies, LT;Moretti, PAB;Anderson, D;Creek, DJ;Powell, JA;Pitson, SM; [2022]
Scientific reports 12(1) 454
Al-Mardeai, S;Elnajjar, E;Hashaikeh, R;Kruczek, B;Van der Bruggen, B;Al-Zuhair, S; [2022]
Molecules (Basel, Switzerland) 27(1)
Murzina, SA;Voronin, VP;Churova, MV;Ruokolainen, TR;Shulgina, NS;Provotorov, DS;Tikhonova, OV;Nemova, NN; [2022]
Biomolecules 12(1)
Ender, A;Grafl, N;Kolberg, T;Findeiss, S;Stadler, PF;Mörl, M; [2022]
RNA (New York, N.Y.)
Looking for an easy, rapid, sensitive, and robust way to quantify endogenous signalling proteins? Inform yourself here on the TR-FRET platform THUNDER.
It doesn't always have to be either or. Read here how endpoint and kinetic measurements can be used synergistically to study protein kinases.
Learn more on successful miniaturization approaches and high-throughput screening attempts in 1536-well plates employing the CRISPR/Cas9 system to knock in Nano luciferase reporter genes.
Covalent drugs are often associated with safety concerns. Nevertheless, a current approach managed to find a new covalent inhibitor for the so far considered un-druggable USP7.
Leave radioactive assays behind: the PHERAstar provides temporal resolution to determine kon and koff rates by TR-FRET binding kinetics.
Find out why the CLARIOstar’s LVF monochromator is perfectly suited for NanoBRET measurements.
Zaida Gloria talks about why she chose the PHERAstar FSX and how this plate reader makes her job easier.
Contract Research Organisations rely on microplate readers for effective hit discovery. Ian Waddell, of Charles River Laboratories, explains what his needs are.
Watch in this customer story how Almac implemented a hit-finding platform for the discovery of novel inhibitors of DeUBiquitinating enzymes
Claire McWhirter is a Principle Scientist at Artios Pharma. In this video, she talks about developing new DNA damage and repair inhibitors on the CLARIOstar Plus.
Ryan McGarrigle from Agilent develops fluorescence-based microplate assays to measure metabolism in different cell types.
Prince Saforo Amponsah from TU Kaiserslautern talks about how redox species (ROS) are generated and regulated and how the CLARIOstar can help.
Powerful and most sensitive HTS plate reader
Most flexible Plate Reader for Assay Development
Flexible microplate reader with simplified workflows
Upgradeable single and multi-mode microplate reader series
Absorbance plate reader with cuvette port