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Analysis of prostate tumour cell invasion using BD FluoroBlok™ and a BMG LABTECH microplate reader

Claire A. Hart, Noel W. Clarke, Michael D. Brown Paterson Institute for Cancer Research, University of Manchester 12/2006

Conclusion


Using a BMG LABTECH microplate reader with integrated Gas Vent or Atmospheric Control Unit (ACU) for reading the FluoroBlokTM tumour cell invasion system gives results comparable to the traditional invasion assay. Compared with traditional invasion systems involving washing, staining and drying steps the introduced method is fast, saving time and labour.


BMG LABTECH readers are able to take multiple readings over time at a user defined temperature and the Gas Vent or ACU enables an environment conducive to cell proliferation and migration.

The mechanism of prostate cancer metastasis to bone marrow is a complex multistage process. One step is the invasion of circulating cancer cells through the bone marrow endothelial barrier and basement membrane.

 

Assays that measure invasion are traditionally time consuming, inflexible and represent one fixed end point only. The BD FluoroBlok™ tumour cell invasion system is a high throughput alternative. It measures fluorescent cells that penetrate a microplate insert and are afterwards found on the bottom of the plate. It allows for multiple real-time readings of the same inserts before and after introducing stimuli. The inserts are made of a dyed membrane that has a fluorescence blocking range from 490-700 nm to be applicable with a range of fluorophores.

 

The system can be automated with the incorporation of a BMG LABTECH microplate reader, to increase efficiency and high throughput of assays for drug discovery, cell migration, motility and chemotaxis studies.

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