SPECTROstar Omega - UV/Vis absorbance spectrophotometer microplate reader
|UV/Vis absorbance spectra|
The SPECTROstar Omega can record both a photometric spectrum (220-1000 nm) down to a resolution of 1 nm and absorbance values at fixed user-defined wavelengths. Similar to a monochromator, but much faster, the spectrometer allows you to capture the UV/Vis spectrum of a sample within one second per well - no scanning needed. The speed of the spectrometer and the easy work-up in the software provide users with unmatched flexibility that can be used to optimize absorbance settings for all your experiments:
Belonging to the Omega microplate reader series, the SPECTROstar Omega absorbance microplate reader provides the following outstanding features:
- Onboard "smart" injectors dispense reagents and initiate kinetic events even when reading full spectra (e.g. monitoring protein denaturation or the increase of different products in an enzymatic assay over time)
- Precise temperature control up to 65°C and multi-mode shaking capability
- Template manager for transferring standards, building complex data processing protocols and using default templates
- Versatile kinetic software features for endpoint, long-term and fast kinetic measurements
- Real-time kinetic monitoring
- Plate capacity up to 384-well
- "QuickStart" mode allows users to read plates with just 3 mouse clicks
- Stacker and robot compatibility
Unique Gas Control Features
The SPECTROstar Omega, as well as the other microplate readers in the Omega Series, has advanced gas control capabilities. Using the Atmospheric Control Unit (ACU) it is possible to fully regulate O2 and CO2 levels within the microplate reader. The microprocessor controlled unit can regulate CO2 (0 - 20%) and O2 (1 - 19%) independently, making it unnecessary to change gas cylinders or piped gas supplies to reproduce certain physiological conditions needed for cell-based assays. In combination with temperature control and unrivalled shaking options, the microprocessor controlled ACU provides a true ‘walk away’ solution for e.g. long term cell based assays. Alternatively, BMG LABTECH offers a traditional purge gas vent, which is beneficial for assays working with samples sensitive to atmospheric gas. This vent can be used to replace the atmosphere in the reader with an inert gas such as CO2 or Ne
Low Volume and Cuvette based measurements
The SPECTROstar Omega is also compatible with the LVis Plate. The LVIS Plate features 16 microdrop well sites for 2 mL samples in addition to a horizontal position for a standard cuvette. It is also fitted with four NIST traceable optical density filters and a holium oxide filter to determine instrument reproducibility and wavelength accuracy. The LVis plate is also coupled with the latest BMG LABTECH control and analysis software, which allows you to take low volume measurements with ease and confidence.
Upgradeability to multidetection reader:
Due to the absolutely modular concept of the Omega microplate reader series, the SPECTROstar Omega absorbance microplate reader is entirely upgradeable to a multidetection microplate reader. Whenever the need for fluorescence and luminescence based experiments arrives, have the SPECTROstar Omega upgraded to a FLUOstar Omega or even to the POLARstar Omega with Simultaneous Dual Emission and Fluorescence Polarization detection mode.
For more information on the Omega series of microplate readers, click here.
SPECTROstar Omega applications include:
- 260/280 nm DNA and RNA quantitation (AN168)
- Protein quantitation (e.g. Bradford (AN158), Lowry, BCA (AN171)
- Reporter gene assays based on absorbance (e.g. ß-Gal, SEAP)
- Cell proliferation and apoptosis
- ELISA, enzyme immunoassays (e.g. alkaline phosphatase, horseradish peroxidase) (AN169)
- Enzyme activity assays: Monitoring absorbance changes in cellular co-factors NADH and NADPH (AN170) allowing the determination of enzymatic activity
- Reaction optimization (metallation of porphyrin in twelve different solvents) by parallel kinetic studies (AN178)
- Microbial growth (6.542)
- Antioxidant capacity of substances using colorimetry (6.335)
Many applications using the SPECTROstar Omega also profit from using the Atmospheric Control Unit (ACU) for O2 / CO2. The following assays are a few examples of such applications:
- Mammalian Cell Assays
- Stem Cell Assays
- Hypoxia research
- Colorimetric Cell viability assays
- Microbial Cell Growth (OD 600)
- Bacterial Studies (AN 155, AN 189)
The SPECTROstar Omega microplate reader is the perfect instrument to conveniently measure any absorbance assay between 220 and 1000 nm. Either single wavelengths or the whole spectrum can be measured in less than 1 sec per well.
For a list of application notes that can be performed on the SPECTROstar Omega absorbance microplate reader, please click here.
|Detection Modes||UV/Vis Absorbance Spectra|
|Measurement Modes ||Endpoint and Kinetic measurements|
|Microplate Formats||6 to 1536-well plates, user-definable|
|Microplate Carrier||Robot compatible|
|Light Sources||High energy xenon flash lamp|
|Spectral Range||220 - 1000 nm for ABS|
|Sensitivity*||ABS with Spectrometer|
Spectral range: 220 to 1000 nm
Full spectrum captured in < 1 s/well
Selectable spectral resolution: 1 nm, 2 nm, 5 nm, 10 nm
OD range: 0 to 4 OD
Accuracy: < 1% at 2 OD
Precision: < 0.5% at 1 OD and < 0.8% at 2 OD
Reproducibility: ±0.005 OD for 0 - 2 OD range
|Read Times||Dependent on test definition|
|Reagent Injection||Up to 2 built-in reagent injectors|
Injection at measurement position (6 to 384-well)
Individual injection volumes for each well (3 to 350 µL)
Variable injection speed up to 420 µL / s
Up to four injection events per well
Reagent back flushing
|Shaking||Linear, orbital, and double-orbital with user-definable time and speed |
|Purge Gas Vent||System to inject an atmosphere or to pull a vacuum into the reader|
|Incubation||+5°C above ambient up to 45°C or 65°C|
|Software||Multi-user Reader Control and
MARS Data Analysis Software included, FDA 21 CFR Part 11 compliant|
|Dimensions||Width: 44 cm, depth: 48 cm, height: 30 cm; weight: 28 kg|
|LVis Plate||Sample Capacity: Sixteen separate microdrop wells for 2 µL samples; One standard cuvette position for up to 1 mL samples. |
Quality Control Internal Standards (optional): Four NIST traceable optical density filters (approximate values of 0.1, 0.3, 0.6 and 1.0 OD); One holmium oxide filter for wavelength accuracy
Dimensions: Conforms to SBS standards for microplates.
|Atmospheric Control Unit (ACU)||Oxygen Regulation: 1 - 19% using nitrogen to purge.|
Carbon Dioxide Regulation: 0 - 20% using 100% moisture free carbon dioxide to purge.
Dimensions (ACU only): Width: 44 cm, height: 17 cm and depth: 19 cm; Weight: 5 kg
|Stacker||Magazines for up to 50 plates - continuous loading feature|
|THERMOstar||Microplate Incubator and Shaker|
|Upgrades||Upgrades to include options such as additional detection modes, reagent injectors, extended temperature control, etc. are available. Please contact your local representative for more information.|
|*||LOD = 3 x SD (20 blanks) / slope (6 pt std curve)|
Microplatets: clear for ABS
96 = 96-well microplates
384sv = 384-well small volume microplates
384g = 384-well glass bottom microplates
Not all of the features mentioned above are available in the basic units or in all detection modes. Optional accessories may be necessary to perform certain assays. Please contact your local distributor for details.
Click on the readers below to choose the instruments that you would like to compare to the SPECTROstar Omega. Then press "Go" to start the comparison.
Please note that the order in which you click the products will be the order in which the products are shown.
HTRF is a registered trademark of Cisbio International.
LanthaScreen is a registered trademark of Invitrogen Corp.
AlphaScreen, AlphaLisa, Surefire, DELFIA, and LANCE are registered trademarks of PerkinElmer, Inc.
Transcreener is a patented technology of BellBrook Labs.