Screen for enzyme-interacting compounds with Transcreener® certified BMG LABTECH readers
The universal Transcreener® assays from BellBrook Labs measure byproducts of common biological enzymatic reactions. Four competitive immunoassays for the direct detection of ADP, AMP/GMP, UDP and GDP are available. Quantification of these nucleotides allows the calculation of enzymatic activity of e.g. kinases or methyltransferases.
Strict Transcreener® validation criteria make sure that the detecting instrumentation fulfills all requirements in terms of performance (Assay Window and Z´value) and read times (a 384w microplate must be read < 5 min).
The BMG LABTECH microplate readers PHERAstar®FSX, CLARIOstar® and POLARstar® Omega all achieved the Transcreener® certification.
Versatile fluorescent detection
The Transcreener ADP2 kinase assay use far-red dyes and can be measured using three different fluorescent readouts:
- Transcreener FP (Fluorescence Polarization)
- Transcreener FI (Fluorescence Intensity)
- Transcreener TR-FRET (Time-Resolved Fluorescence Energy Transfer)
BMG LABTECH's readers are certified for all Transcreener assays.
Meera Kumar from Bellbrook Labs says: "A Z` of 0.84 was obtained at 10% conversion when running a 10 μM ATP/ADP standard curve in FI format while the TR-FRET and FP format gave a Z` of 0.89 at 10% conversion when running a 10 μM ATP/ADP standard curve. We are proud to add the PHERAstar FSX to our growing list of Transcreener certified instruments."
The boxes below lead on to Transcreener related products and application notes.