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Quant-iT PicoGreen dsDNA assay for nucleic acid quantification

Silke Angersbach, Ron Earp, Mark Gröne, Franka Maurer BMG LABTECH 12/2014

The quantification of nucleic acids samples is a major need in any genetic and molecular biology laboratory. Next to well-known UV absorbance assays (e.g. 260/280 nm), a lot of different fluorescence intensity based DNA quantification assays are offered. These assays are not only more sensitive than absorbance assays, they are also able to differentiate between double-stranded and single-stranded DNA, as well as RNA.

 

Aside from sensitivity and specificity, the demand for very low volume measurements is emerging. This is challenging for assays as well as for the detection instrumentation. In this application note we present DNA quantification data obtained on the low volume LVis Plate, as well as 96- and 384-well microplates.

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