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Promega's multiplexed cell viability and apoptosis assays

Tracy Worzella, Brad Larson Promega Corp. 12/2014

Measuring cell viability and death mechanisms is indispensable for testing new compounds, treatment regiments and other toxicological questions. Since cell-culture experiments are cost- and time-consuming the combination of assays is desirable to maximize data output of an experiment. Here, we present the multiplexing of cell viability with either apoptosis assays or with a reporter system indicating living cells, to obtain cell number-corrected viability values.

 

Using the fluorescent CellTiter-Blue™ assay which measures the cellular redox potential with the luminescent Caspase-Glo® 3/7 assay indicated the loss of viability and induction of apoptosis in Jurkat cellsupon addition of the Fas-Ligand, an apoptosis-inducer. Similar results were obtained using CellTier-Blue® with Apo-ONE, a substrate cleaved by caspases to a fluorescent molecule.

 

Relating viability measured by CellTiter-Glo® to a live cell reporter luciferase assay resulted in a stable viability signal in undisturbed cells irrespective of cell proliferation.

 

BMG LABTECH multi-mode plate readers offer the required sensitivity and flexibility to read multiple assays in one well.

Compatible readers

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