Miniaturization of a cell-based TNF-α AlphaLISA assay using Echo liquid handler and the PHERAstar FS

Carl Peters (1), Bonnie Edwards (2) (1) BMG LABTECH, (2) Labcyte 12/2014

Miniaturization for screening purposes presents an attractive scenario in which drug discovery can take place while saving on expenditure of target compounds and detection reagents.

Here we describe the use of the PHERAstar® FS microplate reader from BMG LABTECH to detect a TNFα AlphaLISA® assay which has been miniaturized by the Echo® Liquid Handler from Labcyte. The assay was successfully measured by using only 2 µl in 1536-well microplates.

Compatible readers

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