Luminescent ABEL antioxidant test-kit with PHOLASIN for vitamin c type antioxidants

Bernd Hipler (1), Jan Knight (2) 1) BMG LABTECH, 2) Knight Scientific Ltd. 02/2005

Reactive oxygen species (ROS) are essential intermediates in oxidative metabolism. Nonetheless, when generated in excess, ROS can damage cells by peroxidizing lipids and disrupting structural proteins, enzymes and nucleic acids. ROS can be measured using Pholasin®, the photoprotein of the marine rockboring bioluminescent mollusk. The protein glows upon activation by free radicals and is used in the ABEL® Antioxidant kit.

The light emitted by the probe in the presence of ROS can be detected by all BMG LABTECH multi-mode readers. Using them with integrated reagent injectors allowed for determination of the antioxidative capacity of ascorbate and of natural plant extracts. The luminescence detection was done very fast due to the onboard injectors and with a high precision and reproducibility.

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