Lonza's kinetic kit for endotoxin detection using BMG LABTECH's microplate reader and MARS data analysis
Endotoxins (Lipopolysaccharides) are found in preparations of DNA or proteins produced by gram-negative bacteria. Traces of endotoxins are sufficient to induce an inflammatory response. Thus, preparations need to be tested for endotoxins prior to in vitro or in vivo use. LONZA provides a kit that uses the activation of an enzyme found in the blood of horseshoe crabs by endotoxins. The activated enzyme cleaves a colorless peptide and releases the absorbing p-nitroaniline. The time to reach an absorbance threshold is negative proportional to the amount of endotoxin and is used for endotoxin quantitation. Using standards for a calibration curve between 0.005-5.0 endotoxic units results in a linear curve that can be used to determine endotoxin concentration of unknown samples.