De- and re-oxygenation of plate readers' measurement chamber enables in vitro ischemia/reperfusion research
Oxygen supply is a critical factor adjudicating on health and disease. Rapid changes in oxygen occur if the blood flow is impaired in tissues. Although the blockage of blood vessels is associated with nutrient and oxygen deprivation (ischemia) and causes stroke and myocardial infarction, recovery of the blood stream (reperfusion) is even more deleterious as the rapid increase of oxygen induces reactive oxygen species and inflammation.
The Atmospheric Control Unit (ACU) of the CLARIOstar microplate reader was developed to support research on biological effects of oxygen dysregulation. It sustains hypoxic conditions, rapidly decreases oxygen to mimic ischemia and rapidly re-oxygenates the measurement chamber to simulate reperfusion. The course of oxygen and carbon dioxide concentrations is independently programmable by the user. The profile of oxygen deprivation and re-oxygenation is executed automatically by the ACU while the biological impact is recorded by the microplate reader.
The novel oxygen ramping feature enables in vitro studies of ischemia/reperfusion in high throughput and in real-time. Along with this unique feature, the CLARIOstar and its LVF Monochromator™ offer high flexibility and sensitivity in all detection modes. The possibility to conveniently analyse multiple parameters in parallel (multiplexing) ensures the maximum output of each experiment.
Find below some application note examples for ischemia/reperfusion research and the link to our products.