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Internalisation of fluorescently labelled phagocytosis targets quantified by a BMG LABTECH microplate reader

Abigail Taylor, Louise Donnelly National Heart and Lung Institute, Imperial College London 05/2007

Phagocytosis involves ingestion and destruction of potentially pathogenic particles and forms a major part of the innate immune system. Traditionally, phagocytosis was determined by time consuming methods such as radioactive assays, flow cytometry or microscopy.

 

In this study, the BMG LABTECH microplate reader quantified the internalization of fluorescently labelled phagocytosis targets by monocyte derived macrophages and alveolar macrophages.

 

Using the assay, we found that monocyte derived macrophages can phagocytose polystyrene beads in a comparable manner to alveolar macrophages, and therefore are a suitable model to study phagocytosis. The BMG LABTECH microplate reader provides an accurate, consistent method for quantifying macrophage phagocytosis that is significantly more time efficient than previously employed methods.

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