Homogenous IgG AlphaLISA assay performed on BMG LABTECH's PHERAstar FS
Immunoglobulins or antibodies are proteins that are involved in the immune response. They bind with high affinity at exogenous substances (antigens). Immunoglobulins (Ig) are classified according to the structure. The most present antibody in plasma is IgG. It is part of the secondary antibody response and is built 3 weeks after antigen recognition.
The AlphaLISA® technology is based on laser excitation of Donor beads at 680 nm which induces singlet oxygen formation. Singlet oxygen lives shortly (4 μs in aqueous solutions) and, thus diffuses no more than 200 nm. When a biomolecular interaction brings the Donor and Acceptor beads in proximity, the singlet oxygen reaches the Acceptor bead and a cascade of chemical reactions is initiated producing a luminescence signal.
The IgG AlphaLISA® detection kit was successfully performed on the PHERAstar® providing the sensitivity stated by the manufacturer and reaching Z' values >0.9.