|Time-resolved fluorescence |
|Laser-based AlphaScreen® / AlphaLISA®|
Based on the highly successful HTS microplate reader PHERAstar, the PHERAstar Plus
offers full modularity in all modes and features Advanced HTRF®
/ TR-FRET, Advanced Luminescence and Advanced Fluorescence Polarization reading modes by integrating the latest technological advancements in optics and electronics. The PHERAstar Plus
is the premier multidetection HTS microplate reader with Simultaneous Dual Emission (SDE) detection in all modes. No matter what reading technology you use in your laboratory, the PHERAstar Plus
will provide you with the best sensitivity and the fastest reading speed on the market.
The compact and versatile PHERAstar Plus
microplate reader is able to perform all leading non-isotopic detection technologies including:
- Fluorescence Intensity - including FRET
- Fluorescence Polarization - including FarRed™ and Transcreener®
- Time-Resolved Fluorescence - including DELFIA®
- TR-FRET - including HTRF®, LanthaScreen™, LANCE®
- Laser-based AlphaScreen® / AlphaLISA®
- Absorbance UV/Vis
The PHERAstar Plus
is the ONLY HTS microplate reader on the market with four photomultiplier tubes (PMTs) optimized and automatically selected for specific reading modes. This ensures that your assay is read with highest sensitivity, a feature that no other microplate reader offers.
Due to the unique modular optical system, sequential dual excitation, simultaneous dual emission, and ratiometric calculations, such as for FRET and BRET, the PHERAstar Plus
performs all assays with ease and speed. BMG LABTECH offers assay optimized and barcoded optic modules for your applications with appropriate filters, mirrors, beamsplitters, dichroics or polarizers incorporated into one hand-held module. Once inserted, the PHERAstar Plus
automatically selects the proper module for your assay protocol. Never worry about what dichroic, aperture, or filter is installed in your microplate reader.
The PHERAstar Plus
provides superior sensitivity, speed and dynamic range. Not only does it support endpoint readings, but is also capable of kinetic measurements and well scanning with better control and flexibility than competing readers.
- TR-FRET decay monitoring - can collect TR-FRET curves with 2 µs resolution - in real time
- Real-time display - get kinetic curves as the data is collected. View raw data, graphs, color pictorials - all in real time as the machine is running
- Well scanning up to 30 x 30 data points per well with evaluator to allow you to select points or areas to use for quantitation
- Kinetic data can be collected as quickly as 50 reading points per second or as slowly as one measurement every 2 1/2 hours.
No detection mode is compromised to accommodate the multifunctionality of the PHERAstar Plus
. The PHERAstar Plus
can read 6-1536 well plates with uncompromised sensitivity, - no need for special apertures or optics. Assay flexibility is further enhanced by precise temperature control to 45°C as well as multi-mode shaking capabilities. Automatic Z-height, gain adjustment, and plate mapping as well as the ability to export data in ASCII format complete the package.
The Stacker is designed for integration with the PHERAstar Plus
minimizing microplate handling. It provides loading, unloading, restacking and continous feeding of up to 50 microplates.
The PHERAstar Plus comes with BMG LABTECH's established Reader Control and comprehensive MARS Data Analysis Software with significant ease-of -use enhancements such as S/N, Z', and many other standard calculations. The Wizard creates an easy step-by-step calculation of a standard curve and the Template Manager is an excellent tool for building complex data processing protocols. Thus user-defined data analysis is done simply with one click of the mouse. Improved robotic integration capabilities, digital signature and the 21 CFR part 11 compliance of MARS complete this software package.