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PHERAstar Plus — The Flagship for HTS


Based on the highly successful HTS microplate reader PHERAstar, the PHERAstar Plus offers full modularity in all modes and features Advanced HTRF® / TR-FRET, Advanced Luminescence and Advanced Fluorescence Polarization reading modes by integrating the latest technological advancements in optics and electronics. The PHERAstar Plus is the premier multidetection HTS microplate reader with Simultaneous Dual Emission (SDE) detection in all modes. No matter what reading technology you use in your laboratory, the PHERAstar Plus will provide you with the best sensitivity and the fastest reading speed on the market.

pheraplusbrochureThe compact and versatile PHERAstar Plus microplate reader is able to perform all leading non-isotopic detection technologies including:


Fluorescence Intensity - including FRET
Fluorescence Polarization - including FarRed™ and Transcreener®
Time-Resolved Fluorescence - including DELFIA®
TR-FRET - including HTRF®, LanthaScreen™, LANCE®
Laser-based AlphaScreen® / AlphaLISA®
Luminescence
Absorbance UV/Vis

The PHERAstar Plus is the ONLY HTS microplate reader on the market with four photomultiplier tubes (PMTs) optimized and automatically selected for specific reading modes. This ensures that your assay is read with highest sensitivity, a feature that no other microplate reader offers.


Comapre to


Due to the unique modular optical system, sequential dual excitation, simultaneous dual emission, and ratiometric calculations, such as for FRET and BRET, the PHERAstar Plus performs all assays with ease and speed. BMG LABTECH offers assay optimized and barcoded optic modules for your applications with appropriate filters, mirrors, beamsplitters, dichroics or polarizers incorporated into one hand-held module. Once inserted, the PHERAstar Plus automatically selects the proper module for your assay protocol. Never worry about what dichroic, aperture, or filter is installed in your microplate reader.

The PHERAstar Plus provides superior sensitivity, speed and dynamic range. Not only does it support endpoint readings, but is also capable of kinetic measurements and well scanning with better control and flexibility than competing readers.

• TR-FRET decay monitoring - can collect TR-FRET curves with 2 µs resolution - in real time
• Real-time display - get kinetic curves as the data is collected. View raw data, graphs, color pictorials - all in real time as the machine is running
• Well scanning up to 30 x 30 data points per well with evaluator to allow you to select points or areas to use for quantitation
• Kinetic data can be collected as quickly as 50 reading points per second or as slowly as one measurement every 2 1/2 hours.

No detection mode is compromised to accommodate the multifunctionality of the PHERAstar Plus. The PHERAstar Plus can read 6-1536 well plates with uncompromised sensitivity, - no need for special apertures or optics. Assay flexibility is further enhanced by precise temperature control to 45°C as well as multi-mode shaking capabilities. Automatic Z-height, gain adjustment, and plate mapping as well as the ability to export data in ASCII format complete the package.

The Stacker is designed for integration with the PHERAstar Plus minimizing microplate handling. It provides loading, unloading, restacking and continous feeding of up to 50 microplates.
 


With the PHERAstar Plus, BMG LABTECH offers a unique combination of features to support all major existing applications as well as your future needs. Applications include:pheraplus-video


        • Protein-protein interactions
        • Genotyping
        • Molecular binding assays
        • Receptor ligand binding
        • DNA quantification
        • Enzyme activity and kinetic assays
        • Cell based assays
        • Reporter gene assays
        • Protein quantification
        • G-coupled protein receptors (gpcr)
        • And many more assays (see applications page)


For BMG LABTECH application notes that can be performed on the PHERAstar Plus, please click here.

To download the PHERAstar Plus brochure, please click here.


BMG LABTECH's PHERAstar Plus microplate reader has been granted the LanthaScreen Certified Plus, the highest status based on criteria established in the Invitrogen LanthaScreen certification program. For more information on LanthaScreen, please visit the Invitrogen Corp website: www.invitrogen.com.

The PHERAstar Plus is HTRF® certified from Cisbio international. For more information about HTRF® and the TR-FRET technology in general, please see this website: www.htrf.com

The PHERAstar Plus has received Transcreener® validation. For more information about Transcreener® technology, please visit http://www.bellbrooklabs.com/transcreener.html

Certification Logo HTRFLanthaScreen certificationtranscreener certificationtranscreener fp certificationtranscreener tr fret certification

 


HTRF is a registered trademark of Cisbio international.
Transcreener is a patented technology of BellBrook Labs.
LanthaScreen and FarRed are trademarks of Invitrogen Corp.
AlphaScreen, AlphaLISA, DELFIA, and LANCE are registered trademarks of PerkinElmer, Inc.