PHERAstar Plus — The flagship microplate reader for HTS

		Fluorescence intensity
		Fluorescence polarization
		Time-resolved fluorescence
		TR-FRET
		Laser-based AlphaScreen® / AlphaLISA®
		Luminescence
		Absorbance

Based on the highly successful HTS microplate reader PHERAstar, the PHERAstar Plus offers full modularity in all modes and features Advanced HTRF^® / TR-FRET, Advanced Luminescence and Advanced Fluorescence Polarization reading modes by integrating the latest technological advancements in optics and electronics. The PHERAstar Plus is the premier multidetection HTS microplate reader with Simultaneous Dual Emission (SDE) detection in all modes. No matter what reading technology you use in your laboratory, the PHERAstar Plus will provide you with the best sensitivity and the fastest reading speed on the market.

The compact and versatile PHERAstar Plus microplate reader is able to perform all leading non-isotopic detection technologies including:

• Fluorescence Intensity - including FRET
• Fluorescence Polarization - including FarRed™ and Transcreener^®
• Time-Resolved Fluorescence - including DELFIA^®
• TR-FRET - including HTRF^®, LanthaScreen™, LANCE^®
• Laser-based AlphaScreen^® / AlphaLISA^®


• Luminescence
• Absorbance UV/Vis

The PHERAstar Plus is the ONLY HTS microplate reader on the market with four photomultiplier tubes (PMTs) optimized and automatically selected for specific reading modes. This ensures that your assay is read with highest sensitivity, a feature that no other microplate reader offers.

Due to the unique modular optical system, sequential dual excitation, simultaneous dual emission, and ratiometric calculations, such as for FRET and BRET, the PHERAstar Plus performs all assays with ease and speed. BMG LABTECH offers assay optimized and barcoded optic modules for your applications with appropriate filters, mirrors, beamsplitters, dichroics or polarizers incorporated into one hand-held module. Once inserted, the PHERAstar Plus automatically selects the proper module for your assay protocol. Never worry about what dichroic, aperture, or filter is installed in your microplate reader.

The PHERAstar Plus provides superior sensitivity, speed and dynamic range. Not only does it support endpoint readings, but is also capable of kinetic measurements and well scanning with better control and flexibility than competing readers.

• TR-FRET decay monitoring - can collect TR-FRET curves with 2 µs resolution - in real time


• Real-time display - get kinetic curves as the data is collected. View raw data, graphs, color pictorials - all in real time as the machine is running


• Well scanning up to 30 x 30 data points per well with evaluator to allow you to select points or areas to use for quantitation


• Kinetic data can be collected as quickly as 50 reading points per second or as slowly as one measurement every 2 1/2 hours.

No detection mode is compromised to accommodate the multifunctionality of the PHERAstar Plus. The PHERAstar Plus can read 6-1536 well plates with uncompromised sensitivity, - no need for special apertures or optics. Assay flexibility is further enhanced by precise temperature control to 45°C as well as multi-mode shaking capabilities. Automatic Z-height, gain adjustment, and plate mapping as well as the ability to export data in ASCII format complete the package.

The Stacker is designed for integration with the PHERAstar Plus minimizing microplate handling. It provides loading, unloading, restacking and continous feeding of up to 50 microplates.

The PHERAstar Plus comes with BMG LABTECH's established Reader Control and comprehensive MARS Data Analysis Software with significant ease-of -use enhancements such as S/N, Z', and many other standard calculations. The Wizard creates an easy step-by-step calculation of a standard curve and the Template Manager is an excellent tool for building complex data processing protocols. Thus user-defined data analysis is done simply with one click of the mouse. Improved robotic integration capabilities, digital signature and the 21 CFR part 11 compliance of MARS complete this software package.

PHERAstar Plus applications include:

• Protein-protein interactions using fluorescence polarization (1.144)


• Genotyping (2.1244, 2.1028, AN160)


• Molecular binding assays (3.224)


• Enzyme activity and kinetic assays (2.1201, 6.328, 6.404)


• TR-FRET assays including HTRF® (3.195, AN209), LanthaScreen™(3.228, 3.123) and LANCE®


• Reporter gene assays using luminescence (4.753, 4.603)


• G-coupled protein receptors (GPCR) (AN177)


• DNA and protein quantification

With the PHERAstar Plus, BMG LABTECH offers a unique combination of features to support all major existing applications as well as your future needs.

For a list of BMG LABTECH application notes that can be performed on the PHERAstar Plus, please click here.

HTRF is a registered trademark of Cisbio international.
LanthaScreen is a trademark of Invitrogen Corp.
Lance is a registered trademark of Perkin Elmer Inc.

Detection Modes	Fluorescence Intensity - including FRET Fluorescence Polarization / Anisotropy High-End AlphaScreen®/AlphaLISA® Luminescence - including BRET Time-Resolved Fluorescence - including TR-FRET UV/Vis Absorbance
Measurement Modes	Top reading Endpoint and Kinetic measurements Sequential Multi Excitation measurements Sequential Multi Emission measurements Simultaneous Dual Emission measurements Real-time ratiometric measurements Well Scanning
Certified for	HTRF® LanthaScreen® Certified Plus Transcreener® far red FP Transcreener® red FI Transcreener® red TR-FRET
Microplate Formats	Up to 1536-well plates, user-definable
Microplate Carrier	Robot compatible
Light Sources	High energy xenon flash lamp Dedicated Laser for AlphaScreen® / AlphaLISA®
Detectors	Four photomultiplier tubes, optimized for different detection modes
Wavelength Selection	Optic Modules: Up to five applicaiton specific modules (includes all filters, dichroics, etc. for an application)
Optic Modules Capacity	Up to five application specific Optic Modules built in
Optical Path Guides	Top: Enclosed, free-air optical light path duided by motor-driven mirrors and dichroics
Z-Adjustment	Automatic focal height adjustment (0.1 mm resolution)
Spectral Range	230 - 750 nm or 230 - 900 nm  for ABS, FI, FP, TRF, TR-FRET 230 - 750 nm for LUM
Sensitivity*	FI < 0.5 pM (< 10 amol/well Fluorescein, 384sv, 20 μL) < 2.0 pM (< 16 amol/well Fluorescein, 384sv, 20 μL) FP < 1.0 mP SD at 1 nM Fluorescein, 384sv, 20 μL < 3.0 mP SD at 1 nM Fluorescein, 1536, 3 μL TRF < 25 fM (< 0.5 amol/well Europium, 384sv, 20 μL) < 100 fM (< 0.8 amol/well, Europium, 1536, 3 μL) HTRF® Reader Control Kit (Eu) after 18h (384sv, 20 μL) Delta F > 1100 % (High Calibrator) Delta F > 25 % (Low Calibrator) LUM < 0.5 pM (< 10 amol / well ATP (384sv, 20 μL) AlphaScreen® < 6 pM (< 100 amol/well P-Tyr-100, 384sv, 17 μL) ABS Dynamic range: ±0.000 - 4.000 OD ± 0.005 OD for OD range 0.0 to 2.0 (384) ± 0.010 OD for OD range 2.0 to 3.0 (384)
Read Times	1 flash 19 s (384),  37 s (1536) 10 flashes 41 s (384), 1 min 53 s (1536) 50 flashes 1 min 35 s (384), 5 min 29 s (1536)
Shaking	Linear, orbital, and double-orbital with user-definable time and speed
Incubation	+5°C above ambient up to 45°C
Software	Multi-user Reader Control and MARS Data Analysis Software included, FDA 21 CFR Part 11 compliant
Dimensions	Width: 44 cm, depth: 48 cm, height: 42 cm; weight: 36 kg
Accessories	Optional Accessories
Stacker	Magazines for up to 50 plates - continuous loading feature
THERMOstar	Microplate Incubator and Shaker
Optic Modules	Available for all applications
Upgrades	Upgrades to include options such as additional detection modes, etc. are available. Please contact your local representative for more information.
*	LOD = 3 x SD (20 blanks) / slope (6 pt std curve) AlphaScreen® P-Tyr-100 assay kit, PerkinElmer, #6760620C Microplatets: white for LUM, AlphaScreen®, TRF; black for FI, FP; clear for ABS 384sv = 384-well small volume microplates 1536 = 1536-well microplates

Not all of the features mentioned above are available in the basic units or in all detection modes. Optional accessories may be necessary to perform certain assays. Please contact your local distributor for details.

Additional Accessories:
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Additional Links: Applications Compatibility BMG LABTECH Software

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